Investigation of regenerative and tiss ue-specific activity of tot al RNA of bone marrow cells
https://doi.org/10.15825/1995-1191-2018-3-64-69
Abstract
Aim. To establish the ability of the total RNA extracted from the body’s bone marrow cells (BMCs), in which liver tissue was damaged, to serve as a carrier of targeted regenerative signals to this organ.
Materials and methods. By method of adoptive transfer in rats (n = 37) the mitotic and proliferative activity of liver and kidney cells were studied in intact recipients after intraperitoneal injection: the mononuclear BMCs – 2,5×106; 5,0×106; 3,5×107 cells – group 1 and the total RNA of the same BMCs (30μg/100g of weight) – group 2 from donors in 12 hours after 70–75% of hepatectomy; in group 3 (control), a saline solution was injected. RNA from BMCs was extracted by the method developed by the «Evrogen» firm (Russia) with the reagent Extract RNA.
Results. In group 2 in 48 and 72 h. there was the increasing of mitotic and proliferative cell activity in the liver, but not in the kidneys (control of the specificity of regenerative signals); in group 1 there was no transfer of regenerative signals to these organs.
Conclusion. The authors believe that the total RNA from BMCs, activated by hepatectomy, accumulates targeted (hepatospecific) regeneration signals, but they are perceived only when RNA has been obtained by the damaged tissue.
About the Authors
Z. Z. Gonikova
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Address: 1, Shchukinskaya st., Moscow, 123182, Russian Federation. Tel. (966) 188-33-33
A. O. Nikolskaya
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
L. A. Kirsanova
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
M. Yu. Shagidulin
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
I.M. Sechenov First Moscow State Medical University of the Ministry of Healthcare of the Russian Federation (Sechenovsky University)
Russian Federation
Russian Federation
N. A. Onishchenko
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
V. I. Sevastyanov
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
References
1. Бабаева АГ, Геворкян НМ, Зотиков ЕА. Роль лимфоцитов в оперативном изменении программы развития тканей. М.: Изд. РАМН, 2009: 107. Babaeva AG, Gevorkyan NM, Zotikov EA. Rol’ limfocitov v operativnom izmenenii programmy’ razvitiya tkanej. M.: Izd. RAMN, 2009: 107.
2. Бабаева АГ, Тишевская НВ, Геворкян НМ. О морфогенетических свойствах РНК лимфоидных и стволовых клеток при восстановительных процессах // Рос. акад. наук, Науч.-исслед. ин-т морфологии человека. М., 2016: 272. Babaeva AG, Tishevskaya NV, Gevorkyan NM. O morfogeneticheskih svojstvah RNK limfoidnyh i stvolovyh kletok pri vosstanovitel’nyh processah // Ros. akad. nauk, Nauch.-issled. in-t morfologii cheloveka. M., 2016: 272.
3. Тишевская НВ, Бабаева АГ, Геворкян НМ. Роль лимфоцитарных РНК в межклеточном информационном обмене и регуляции регенеративных процессов. Рос. физиол. журнал им. И.М. Сеченова. 2016; 102 (11): 1280–1301. Tishevskaya NV, Babaeva AG, Gevorkyan NM. Rol’ limfocitarnyh RNK v mezhkletochnom informacionnom obmene i regulyacii regenerativnyh processov. Ros. fiziol. zhurnal im. I.M. Sechenova. 2016; 102 (11): 1280–1301.
4. Gregorini M, Bosio F, Rocca C, Corradetti V et al. Mesenchymal stromal cells reset the scatter factor system and cytokine network in experimental kidney transplantation. BMC Immunol. 2014 Oct 3; 15: 44. DOI: 10.1186/s12865-014-0044-1.
5. Kaibori M, Adachi Y, Shimo T, Ishizaki M et al. Stimulation of liver regeneration after hepatectomy in mice by injection of bone marrow mesenchymal stem cells via the portal vein. Transplant. Proc. 2012 May; 44 (4): 1107–1109. DOI: 10.1016/j.transproceed.2012.01.088.0044-1.
6. Kantarcıoğlu M, Demirci H, Avcu F, Karslıoğlu Y et al. Efficacy of autologous mesenchymal stem cell transplantation in patients with liver cirrhosis. Turk. J. Gastroenterol. 2015 May; 26 (3): 244–250. DOI: 10.5152/tjg.2015.0074.
7. Reis LA, Borges FT, Simões MJ, Borges AA et al. Bone marrow-derived mesenchymal stem cells repaired but did not prevent gentamicin-induced acute kidney injury through paracrine effects in rats. PLoS One. 2012; 7 (9): e44092. DOI: 10.1371/journal.pone.0044092. Epub 2012 Sep 6.
8. Wan Z, You S, Rong Y, Zhu B, Zhang A et al. CD34+hematopoietic stem cells mobilization, paralleled with multiple cytokines elevated in patients with HBV-related acute-on-chronic liver failure. Dig. Dis. Sci. 2013 Feb; 58 (2):448–457. DOI: 10.1007/s10620-012-2458-z. Epub 2012 Oct 25.
9. Бойко НВ, Голиков АЮ, Тарасов ВА, Матишов ДГ. Роль микро-РНК в регуляции активности генов у эукариот. Вестник южного научного центра РАН. 2011; 7 (3): 69– 78. Bojko NV, Golikov AYu, Tarasov VA, Matishov DG. Rol’ mikro-RNK v regulyacii aktivnosti genov u eukariot. Vestnik yuzhnogo nauchnogo centra RAN. 2011; 7 (3): 69– 78.
10. Prasanth KV, Spector DL. Eukaryotic regulatory RNAs: an answer to the genome complexity conundrum. Genes and Development. 2007; 21: 11–42.
11. Friedman RC, Farh KK, Burge CB, Bartel DP. Most mammalian mRNAs are: conserved targets of micro-RNAs. Genome Res. 2009 Jan; 19 (1): 92–105. DOI: 10.1101/gr.082701.108. Epub 2008 Oct 27.
12. He L, Hannon GJ. Micro-RNAs: small RNAs with a big role in gene regulation. Nat. Rev. Genet. 2004 Jul; 5 (7): 522–531.
13. Тишевская НВ, Геворкян НМ, Бабаева АГ, Захаров ЮМ и др. Влияние суммарной РНК лимфоидных клеток селезенки на эритропоэз при экспериментальной полицитемии. Российский физиол. журнал им. И.М. Сеченова. 2015; 101 (4): 451– 461. Tishevskaya NV, Gevorkyan NM, Babaeva AG, Zaharov YuM i dr. Vliyanie summarnoj RNK limfoidnyh kletok selezenki na ehritropoehz pri ehksperimental’noj policitemii. Rossijskij fiziol. zhurnal im. I.M. Sechenova. 2015; 101 (4): 451–461.
14. Бабаева АГ, Геворкян НМ, Тишевская НВ, Головкина ЛЛ и др. О гемопоэтических свойствах рибонуклеиновой кислоты лимфоцитов периферической крови больных истинной полицитемией и здоровых доноров. Онкогематология. 2015; 2 (10): 58–626. Babaeva AG, Gevorkyan NM, Tishevskaya NV, Golovkina LL i dr. O gemopoehticheskih svojstvah ribonukleinovoj kisloty limfocitov perifericheskoj krovi bol’nyh istinnoj policitemiej i zdorovyh donorov. Onkogematologiya. 2015; 2 (10): 58– 626.
15. Ельчанинов АВ, Фатхудинов ТХ, Усман НЮ и др. Экспрессия генов цитокинов и факторов роста в печени после субтотальной резекции у крыс. Гены и клетки. 2016; 11 (1): 61–67. El’chaninov AV, Fathudinov TH, Usman NYu i dr. Ehkspressiya genov citokinov i faktorov rosta v pecheni posle subtotal’noj rezekcii u krys. Geny i kletki. 2016; 11 (1): 61–67.
Review
For citations:
Gonikova Z.Z., Nikolskaya A.O., Kirsanova L.A., Shagidulin M.Yu., Onishchenko N.A., Sevastyanov V.I. Investigation of regenerative and tiss ue-specific activity of tot al RNA of bone marrow cells. Russian Journal of Transplantology and Artificial Organs. 2018;20(3):64-69. (In Russ.) https://doi.org/10.15825/1995-1191-2018-3-64-69
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