FEATURES OF ISLET-LIKE CLUSTERS GENERATION IN PANCREATIC DUCTAL CELL MOLOLAYER CULTURING
https://doi.org/10.15825/1995-1191-2012-4-74-79
Abstract
Newborn rabbit pancreatic cell monolayer was obtained as we described earlier.The cultivated epithelial cells were shown by immunofluorescence to express special ductal marker CK19 and were insulin-and glucagon- negative for 10–15 days. A few fusiforms of nestin-positive cells were found in monolayer. Over 2 weeks in serum-free medium the plaques of epithelial cells became crowded and formed 3-dimentional structures – islet- like clusters. Islet-like clusters contain some insulin- and glucagon-positive cells recognized by immunohysto- chemistry staining. Pancreatic endocrine cell generation in 3-dimentional structures is discussed.
About the Authors
L. A. Kirsanova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
G. N. Bubentsova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
N. V. Baranova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
N. N. Skaletskiy
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
V. A. Zaydenov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
I. A. Pushkova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
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Review
For citations:
Kirsanova L.A., Bubentsova G.N., Baranova N.V., Skaletskiy N.N., Zaydenov V.A., Pushkova I.A. FEATURES OF ISLET-LIKE CLUSTERS GENERATION IN PANCREATIC DUCTAL CELL MOLOLAYER CULTURING. Russian Journal of Transplantology and Artificial Organs. 2012;14(4):74-79. (In Russ.) https://doi.org/10.15825/1995-1191-2012-4-74-79
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