CELL-ENGINEERING DESIGNS TRANSPLANTED INTO LIVER PROVIDE WITH PROLONGED SUPPORT OF RECOVERY PROCESSES IN DAMAGED LIVER
https://doi.org/10.15825/1995-1191-2013-2-65-75
Abstract
Aim is to develop a method for a prolonged support of recovery processes in damaged liver. Materials and me- thods. It was carried out 3 groups of experiments on Wistar rats with the modeling of chronic fibrotic liver injury (n = 70): I group control (n = 20); in the II group (n = 20) a suspension of liver cells was transplanted into liver; in the III group (n = 30) cell-engineering designs (CED), which contained liver cells and BM MMSC, enclosed in a heterogeneous biodegradable gel “Sphero®GEL-long” were transplanted into damaged liver. The activity of recovery processes was evaluated by using biochemical and morphological methods in dynamics on 30, 60, 90 and 180 days. Results. It was shown that in the II and III gr. significantly accelerated the recovery processes in damaged livers compared with the I gr. The normalization of biochemical parameters took place in II and III du- ring 30 days instead of 90 days in the I group. However, the normalization of morphological signs of hepatocytes theirs viability and a degree of defibrotic changes in liver were more pronounced and prolonged in the III group. A study showed integration of CED by liver structures with formation of new bile ducts after 90 and 180 days. Conclusion. Higher levels and prolonged periods of recovery processes in damaged liver after CED transplanta- tion were due to the creation of biologically appropriate conditions for prolonged cell activity, included in their structure (donor liver cells and BM MMSC).
About the Authors
M. Y. Shagidulin
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
(Head – academician of RAMSci, prof. S.V. Gautier); Laboratory of Strategy and Monitoring
of The Transplantology Development (Head laboratory – S.M. Khomyakov), Moscow, Russian Federation
N. A. Onischenko
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
(Head – academician of RAMSci, prof. S.V. Gautier); Laboratory of Strategy and Monitoring
of The Transplantology Development (Head laboratory – S.M. Khomyakov), Moscow, Russian Federation
M. E. Krasheninnikov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
I. M. Iljinsky
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
(Head – academician of RAMSci, prof. S.V. Gautier); Laboratory of Strategy and Monitoring
of The Transplantology Development (Head laboratory – S.M. Khomyakov), Moscow, Russian Federation
A. V. Lyundup
Research Institute of Molecular Medicine (Head – prof. V.N. Nikolenko) I.M. Sechenov First Moscow State Medical University (Rector – corresponding member of RAMSci, prof. P.V. Glybochko), Moscow, Russian Federation
V. I. Sevastyanov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
N. P. Mogeiko
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
S. V. Gautier
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs
(Head – academician of RAMSci, prof. S.V. Gautier); Laboratory of Strategy and Monitoring
of The Transplantology Development (Head laboratory – S.M. Khomyakov), Moscow, Russian Federation
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For citations:
Shagidulin M.Y., Onischenko N.A., Krasheninnikov M.E., Iljinsky I.M., Lyundup A.V., Sevastyanov V.I., Mogeiko N.P., Gautier S.V. CELL-ENGINEERING DESIGNS TRANSPLANTED INTO LIVER PROVIDE WITH PROLONGED SUPPORT OF RECOVERY PROCESSES IN DAMAGED LIVER. Russian Journal of Transplantology and Artificial Organs. 2013;15(2):65-75. (In Russ.) https://doi.org/10.15825/1995-1191-2013-2-65-75
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