DEVELOPMENT OF METHODOLOGICAL APPROACHES TO OBTAINING ISLET CELLS BASED ON THE RESULTS OF THE MORPHOLOGICAL ANALYSIS OF THE PANCREAS OF RABBITS OF DIFFERENT AGES
https://doi.org/10.15825/1995-1191-2013-4-98-103
Abstract
Purpose. A comparative morphological analysis of adult pancreas and newborn rabbits as acceptable model for obtaining of islet cell cultures having a low immunogenicity was agoal of this study.
Materials and methods. Pancreas from adult and newborn rabbits and islet cell culture was examined by histological and immunohistochemical techniques.
Results. Shown, the pancreas of adult rabbits contains great amount of exocrine tissue and culturing it does not allow to obtain the purified islets of impurities. By contrast, pancreas of newborn rabbits in which the ratio of the islets and the exocrine tissue is much higher, it is possible to obtain highly purified cultures of islet cells.
Conclusion. Morphological features of newborn rabbit pancreas can use it as a model for obtaining cultures of islet cells having low immunogenicity.
About the Authors
N. N. Skaletskiy
Cell transplantation laboratory (Head – Doctor of med. sci N.N. Skaletskiy) Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation (Director – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
L. A. Kirsanova
Cell transplantation laboratory (Head – Doctor of med. sci N.N. Skaletskiy) Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation (Director – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
N. V. Baranova
Cell transplantation laboratory (Head – Doctor of med. sci N.N. Skaletskiy) Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation (Director – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
G. N. Bubentsova
Cell transplantation laboratory (Head – Doctor of med. sci N.N. Skaletskiy) Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation (Director – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
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Review
For citations:
Skaletskiy N.N., Kirsanova L.A., Baranova N.V., Bubentsova G.N. DEVELOPMENT OF METHODOLOGICAL APPROACHES TO OBTAINING ISLET CELLS BASED ON THE RESULTS OF THE MORPHOLOGICAL ANALYSIS OF THE PANCREAS OF RABBITS OF DIFFERENT AGES. Russian Journal of Transplantology and Artificial Organs. 2013;15(4):98-103. (In Russ.) https://doi.org/10.15825/1995-1191-2013-4-98-103
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