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COMPLEX APPROACH FOR PORTABLE CRYOPRESERVATION OF SEGMENTS OF BLOOD VESSELS WITH POLYDIMETHYLSILOXANE

https://doi.org/10.15825/1995-1191-2018-1-86-95

Abstract

Aim. To develop a safe protocol for cryopreservation of segments of iliac arteries straight after their retrieval from post-mortem donor with the use of polydimethylsiloxane as a coolant and cryoprotectant.

Materials and methods. Eleven segments of iliac arteries were retrieved from post-mortem donor and divided into four groups including control. Based on preliminary heat and cold transfer mathematical modeling and tests with tissueequivalent phantom arterial segments were placed on plastic mounts and cryopreserved by following protocol: groups 1 and 2 were immersed in polydimethylsiloxane and cooled rapidly at 180 °С/min to –75 °С. Group 3 segments were cryopreserved at 1,6 °С/min in PDMS – fi lled cryo-container placed in the freezer at –80 °С. All segments were defrosted by immersion in PDMS at +24 °С and then examined for morphology changes by histological methods and SEM. EDS analysis with the use of AzTech software also was performed for Si – content evaluation. Restricted biomechanical tests were conducted for group 2 segments.

Results. There were no signifi cant morphological differences between segments of the control and cryopreserved groups except for the segment with slow cooling.

Conclusion. Mobile cryopreservation may allow increasing the effi ciency of retrieval of a large number of donor tissues for possible later use in the processing of bioprostheses of blood vessels; or, after decellularization, as well as tissue-specifi c matrices for tissue-engineering blood vessels.

About the Authors

S. E. Lauk-Dubitsky
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation

Lauk-Dubitsky Stanislav Evgenievich

30, Volokolamskoie sh., Moscow, 123182



T. A. Astrelina
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


A. A. Fedyunin
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


I. A. Burkov
The Bauman Moscow State Technical University
Russian Federation
Moscow


E. M. Kildyushov
Bureau of Forensic Medical Examination of the Moscow City Health Department
Russian Federation
Moscow


S. V. Lischuk
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


I. A. Novikov
State Research Institute of Eye Diseases of Russian Academy of Medical Sciences
Russian Federation
Moscow


A. V. Pushkarev
The Bauman Moscow State Technical University
Russian Federation
Moscow


V. A. Brumberg
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


A. V. Shakurov
The Bauman Moscow State Technical University
Russian Federation
Moscow


D. P. Samchuk
Research Institute of Biomedical Chemistry n.a. V.N. Orekhovich
Russian Federation
Moscow


I. V. Kobzeva
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


Yu. B. Suchkova
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


V. A. Nikitina
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


T. V. Karaseva
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


A. Yu. Bushmanov
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


A. S. Samoilov
Federal Medical and Biophysical Center n.a. A.I. Burnazyan
Russian Federation
Moscow


References

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Review

For citations:


Lauk-Dubitsky S.E., Astrelina T.A., Fedyunin A.A., Burkov I.A., Kildyushov E.M., Lischuk S.V., Novikov I.A., Pushkarev A.V., Brumberg V.A., Shakurov A.V., Samchuk D.P., Kobzeva I.V., Suchkova Yu.B., Nikitina V.A., Karaseva T.V., Bushmanov A.Yu., Samoilov A.S. COMPLEX APPROACH FOR PORTABLE CRYOPRESERVATION OF SEGMENTS OF BLOOD VESSELS WITH POLYDIMETHYLSILOXANE. Russian Journal of Transplantology and Artificial Organs. 2018;20(1):86-95. (In Russ.) https://doi.org/10.15825/1995-1191-2018-1-86-95

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ISSN 1995-1191 (Print)