CULTIVATION OF HUMAN LIVER CELLS AND ADIPOSE-DERIVED MESENCHYMAL STROMAL CELLS IN PERFUSION BIOREACTOR
https://doi.org/10.15825/1995-1191-2018-1-70-78
Abstract
Aim: to show the progress of the experiment of cultivation of human liver cells and adipose-derived mesenchymal stromal cells in perfusion bioreactor.
Materials and methods. The cultivation of a cell-engineered construct, consisting of a biopolymer microstructured collagen-containing hydrogel, human liver cells, adipose-derived mesenchymal stromal cells, and William’s E Medium, was performed in a perfusion bioreactor.
Results. On the 7th day large cells with hepatocyte morphology – of a polygonal shape and a centrally located round nucleus, – were present in the culture chambers of the bioreactor. The metabolic activity of hepatocytes in cell-engineered constructs was confi rmed by the presence of urea in the culture medium on the seventh day of cultivation in the bioreactor and by the resorption of a biopolymer microstructured collagen-containing hydrogel.
About the Authors
Yu. В. Basok
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Basok Yulia Borisovna
1, Shchukinskaya st., Moscow, 123182
A. M. Grigoryev
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow
Russian Federation
Moscow
L. A. Kirsanova
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow
Russian Federation
Moscow
N. P. Shmerko
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow
Russian Federation
Moscow
К. M. Khizroev
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow
Russian Federation
Moscow
V. I. Sevastianov
V.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow
Russian Federation
Moscow
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Review
For citations:
Basok Yu.В., Grigoryev A.M., Kirsanova L.A., Shmerko N.P., Khizroev К.M., Sevastianov V.I. CULTIVATION OF HUMAN LIVER CELLS AND ADIPOSE-DERIVED MESENCHYMAL STROMAL CELLS IN PERFUSION BIOREACTOR. Russian Journal of Transplantology and Artificial Organs. 2018;20(1):70-78. (In Russ.) https://doi.org/10.15825/1995-1191-2018-1-70-78
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