Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor
https://doi.org/10.15825/1995-1191-2017-3-81-92
Abstract
Formation of tissue-engineered construct was performed in a specially developed bioreactor. At first, a cellengineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, mesenchymal stromal cells of human adipose tissue (hADMSCs) and induction chondrogenic culture medium was prepared and placed in a perfusion bioreactor. As a result, on the 16th day of the study hADMSCs obtain a flattened shape typical for chondroblasts and demonstrate high proliferative activity with the formation of their own extracellular matrix. Histological analysis of the cultured system indicates the beginning of the formation of a tissue-engineered construct of human cartilage tissue.
About the Authors
V. I. SevastianovRussian Federation
Address: 1, Shchukinskaya st., Moscow, 123182, Russian Federation. Теl. (916) 173-41-84
Yu. B. Basok
Russian Federation
A. M. Grigoriev
Russian Federation
L. A. Kirsanova
Russian Federation
V. N. Vasilets
Russian Federation
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Review
For citations:
Sevastianov V.I., Basok Yu.B., Grigoriev A.M., Kirsanova L.A., Vasilets V.N. Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor. Russian Journal of Transplantology and Artificial Organs. 2017;19(3):81-92. (In Russ.) https://doi.org/10.15825/1995-1191-2017-3-81-92