Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor
https://doi.org/10.15825/1995-1191-2017-3-81-92
Abstract
Formation of tissue-engineered construct was performed in a specially developed bioreactor. At first, a cellengineered construct of human cartilage tissue consisting of biopolymer microstructured collagen-containing hydrogel, mesenchymal stromal cells of human adipose tissue (hADMSCs) and induction chondrogenic culture medium was prepared and placed in a perfusion bioreactor. As a result, on the 16th day of the study hADMSCs obtain a flattened shape typical for chondroblasts and demonstrate high proliferative activity with the formation of their own extracellular matrix. Histological analysis of the cultured system indicates the beginning of the formation of a tissue-engineered construct of human cartilage tissue.
About the Authors
V. I. Sevastianov
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Address: 1, Shchukinskaya st., Moscow, 123182, Russian Federation. Теl. (916) 173-41-84
Yu. B. Basok
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
A. M. Grigoriev
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
L. A. Kirsanova
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
V. N. Vasilets
V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
References
1. Liu M, Yu X, Huang F, Cen S, Zhong G, Xiang Z. Tissue engineering stratified scaffolds for articular cartilage and subchondral bone defects repair. Orthopedics. 2013; 36 (11): 868–873. doi: 10.3928.
2. Stubbs AJ, Howse EA, Mannava S. Tissue engineering and the future of hip cartilage, labrum and ligamentum teres. J. Hip. Preserv. Surg. 2015; 11 (3): 23–29. doi: 10.1093.
3. Sevast’janov VI, Grigor’ev AM, Skaleckij NN, Got’e SV. Razrabotka i jeksperimental’no- klinicheskoe issledovanie tkaneinzhenernoj konstrukcii hrjashha. Transplantologija: itogi i perspektivy. 2014. T. VI. Pod red. S.V. Got’e. M., 2015: 125–130.
4. Sevast’janov VI, Perova NV, Nemec EA, Surguchenko VA, Ponomareva AS. Primery jeksperimental’no-klinicheskogo primenenija biosovmestimyh materialov v regenerativnoj medicine. Biosovmestimye materialy (uchebnoe posobie). Pod red. V.I. Sevast’janova i M.P. Kirpichnikova. M.: MIA, 2011. Chast’ II, glava 3: 237–252. [In Russ].
5. Solov’eva IV, Perova NV, Sevast’janov VI. Vozmozhnosti primenenija biopolimernogo mikrogeterogennogo kollagensoderzhashhego gelja pri travmah i zabolevanijah oporno-dvigatel’nogo apparata. Sovremennaja medicina. 2016; 2: 66–69. [In Russ].
6. Solov’eva IV, Shesternja N, Perova NV, Sevast’janov VI. Kombinirovannoe primenenie biopolimernogo geterogennogo gidrogelja i gialuronovoj kisloty pri OA (pervyj opyt). Vrach. 2016; 1: 12–17. [In Russ].
7. Sevastianov VI, Dukhina GA, Grigoriev AM, Perova NV, Kirsanova LA, Skaletskiy NN et al. The functional effectiveness of a cell-engineered construct for the regeneration of articular cartilage. Russian Journal of Transplantology and Artificial Organs. 2015; 17 (1): 86–96. doi:10.15825/1995-1191-2015-1-86-96. [In Russ, English abstract].
8. Surguchenko VA, Ponomareva AS, Kirsanova LA, Skaleckij NN, Sevastianov VI. The cell- engineered construct of cartilage on the basis of biopolymer hydrogel matrix and human adipose tissue-derived mesenchymal stromal cells (in vitro study). J. Biomed. Mater. Res. 2015; 103A (2): 463–470.
Review
For citations:
Sevastianov V.I., Basok Yu.B., Grigoriev A.M., Kirsanova L.A., Vasilets V.N. Application of tissue engineering technology for formation of human articular cartilage in perfusion bioreactor. Russian Journal of Transplantology and Artificial Organs. 2017;19(3):81-92. (In Russ.) https://doi.org/10.15825/1995-1191-2017-3-81-92
Views:
1351
Email this article 