Morphological evaluation of the quality of decellularized esophagus in rat
https://doi.org/10.15825/1995-1191-2017-2-98-103
Abstract
The main aim of our investigation was analysis and optimization of the existing protocols of esophagus decellularization in rats, identification of parameters affecting the quality of decellularization and intactness of the native properties of esophageal extracellular matrix (ECM). Materials and methods. We developed a modified decellularization protocol based on detergent-enzymatic method using sodium deoxycholate and DNAse. Results. Morphological evaluation of the obtained decellularized matrices demonstrated the absence of cells and cell elements while mechanical properties of ECM were preserved. Conclusion. The developed protocol of esophagus decellularization is a potential basis for obtaining of tissue-engineered esophagus scaffold constructions.
About the Authors
R. Z. Nakokhov
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
E. A. Gubareva
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
E. V. Kuevda
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
A. S. Sotnichenko
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
I. S. Gumenyuk
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
A. Kh. Kade
Department of General and Clinical Pathophysiology, Kuban State Medical University
Russian Federation
Russian Federation
G. M. Mogilnaya
Department of Histology and Embryology, Kuban State Medical University
Russian Federation
Russian Federation
D. P. Puzanov
Laboratory of fundamental research in the field of regenerative medicine,
Kuban State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Nakokhov R.Z., Gubareva E.A., Kuevda E.V., Sotnichenko A.S., Gumenyuk I.S., Kade A.Kh., Mogilnaya G.M., Puzanov D.P. Morphological evaluation of the quality of decellularized esophagus in rat. Russian Journal of Transplantology and Artificial Organs. 2017;19(2):98-103. (In Russ.) https://doi.org/10.15825/1995-1191-2017-2-98-103
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