MICRONEEDLES AS A WAY TO INCREASE THE TRANSDERMAL INSULIN DELIVERY
https://doi.org/10.15825/1995-1191-2016-4-87-92
Abstract
Aim: to prove the possibility of increasing the diffusion of insulin through the skin in vitro with pre-applying microneedles.
Materials and methods. Microemulsion for transdermal therapeutic system of insulin has been used in vitro studies. Genetically engineered human insulin has been used in this research. Applicators with silicon microneedles (40 and 150 microns long) have been used to enhance the diffusion fl ux of drug substance. The dynamics of insulin release from the transdermal therapeutic systems through the rabbit skin has been studied in glass Franz diffusion cells in analyzer diffusion of drugs HDT 1000 (Copley Scientifi c Ltd., UK). Insulin has been labeled with fl uorescein isothiocyanate to separate the insulin absorption spectrum from the spectra of native skin proteins at spectrophotometer measurements.
Results. The amounts of insulin delivered through the skin in vitro after previous application of microneedles of 40 and 150 microns are 282.5 ± 61.1 and 372.3 ± 7.0 microgram, respectively. This is 1.4 and 1.9 times more than in the transdermal system without microneedles.
Conclusion. The conditions for increasing the diffusion of insulin through the skin in a model transdermal therapeutic system with microneedles (length – 150 microns, duration of pre-application – 1 hour) have been found.
About the Authors
E. G. Kuznetsova
V.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Russian Federation
Address: 1, Shchukinskaya St., Moscow, 123182, Russian Federation. Tel. (499) 193-86-62
V. A. Ryzhikova
V.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow, Russian Federation
Russian Federation
Moscow, Russian Federation
L. A. Salomatina
V.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow, Russian Federation
Russian Federation
Moscow, Russian Federation
V. A. Tukmachev
F.V. Lukin Federal Research Institute of Physical Problems
Russian Federation
Moscow, Zelenograd, Russian Federation
Russian Federation
Moscow, Zelenograd, Russian Federation
V. I. Sevastianov
V.I. Shumakov Federal Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian Federation
Russian Federation
Moscow, Russian Federation
Russian Federation
Moscow, Russian Federation
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Review
For citations:
Kuznetsova E.G., Ryzhikova V.A., Salomatina L.A., Tukmachev V.A., Sevastianov V.I. MICRONEEDLES AS A WAY TO INCREASE THE TRANSDERMAL INSULIN DELIVERY. Russian Journal of Transplantology and Artificial Organs. 2016;18(4):87-92. (In Russ.) https://doi.org/10.15825/1995-1191-2016-4-87-92
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