INFLUENCE OF MICROSTRUCTURED COLLAGEN HYDROGEL ON PANCREATIC ISLET CELL CULTURES
https://doi.org/10.15825/1995-1191-2014-1-29-33
Abstract
Aim. A study of the influence of microstructured collagen hydrogel (biomatrix) on survival and growth charac- teristics of islet cell cultures at their co-incubation.
Materials and methods. As a biomatrix, the microstructured collagen hydrogel of linear series Sphero® GEL was used. Islet cell cultures obtained from newborn rabbit pan- creas were inoculated onto the biomatrix surface, covered with growth medium, and placed in a CO2 incubator. Changes occurring with biomatrix and cultures were observed by means of an inverted microscope and histolo- gical studies, including immunohistochemical analysis.
Results. The presence of the microstructured collagen hydrogel matrix during the incubation of floating islet cell cultures promoted long-term preservation of the structural integrity and hormonal activity. Simultaneously the formation of cultures of pancreatic progenitor cells (islet cells precursors) was observed.
Conclusion. Collagen hydrogel has a favorable effect on the formation and survival of islet cell cultures and can be used as a matrix of a tissue-engineered pancreas construct.
About the Authors
L. A. Kirsanova
Laboratory of cell transplantation «V.I. Shumakov Federal Research Center
of Transplantology and Artificial Organs», Moscow, Russian Federation
N. V. Baranova
Laboratory of cell transplantation «V.I. Shumakov Federal Research Center
of Transplantology and Artificial Organs», Moscow, Russian Federation
G. N. Bubentsova
Laboratory of cell transplantation «V.I. Shumakov Federal Research Center
of Transplantology and Artificial Organs», Moscow, Russian Federation
G. N. Skaletskaya
Laboratory of cell transplantation «V.I. Shumakov Federal Research Center
of Transplantology and Artificial Organs», Moscow, Russian Federation
N. V. Perova
Laboratory of tissue engineering and delivery systems, department of biomedical technologies and tissue engineering, «V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs», Moscow, Russian Federation
V. I. Sevastianov
Laboratory of tissue engineering and delivery systems, department of biomedical technologies and tissue engineering, «V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs», Moscow, Russian Federation
N. N. Skaletskiy
Laboratory of cell transplantation «V.I. Shumakov Federal Research Center
of Transplantology and Artificial Organs», Moscow, Russian Federation
References
1. СПИСОК ЛИТЕРАТУРЫ / REFERENCES
2. Биосовместимые материалы (учебное пособие) / Под ред. В.И. Севастьянова и М.П. Кирпичникова. M.: МИА, 2011; 544.
3. Biosovmestimiye materialy (uchebnoe posobie). Editors Sevastianov V.I. and Kirpichnikov M.P. M: MIA, 2011: 544 (in rus).
4. Surguchenko V.A., Ponomareva A.S., Kirsanova L.A., Bubentsova G.N., Skaletskij N.N., Sevastianov V.I. On the possibility of in vitro formation of tissue-engineered construct of cartilage on the basis of cell-engineered con- struct composed of biopolymer hydrogel matrix and hu- man adipose tissue-derived mesenchymal stromal cells. Advanced Metals, Ceramics and Composites (ed. H. Tu, K. Solntsev, R. Zhou), Yunnan Publ. Group Corp., Kun- ming, China, 2013: 242–245.
5. Готье С.В., Шагидулин М.Ю., Онищенко Н.А., Кра- шенинников М.Е., Ильинский И.М., Можейко Н.П., Люндуп А.В., Волкова Е.Н., Петраков К.В., Авра- мов П.В., Перова Н.В., Севастьянов В.И. Коррекция хронической печеночной недостаточности при транс- плантации клеток печени в виде суспензии и клеточ- но-инженерных конструкций (экспериментальное исследование). Вестник РАМН. 2013; 4: 44–51. Gauthier S.V., Shagidulin M.Yu, Onishchenko N.A., Krasheninnikov M.E., Il'inskii I.M., Mazhejko N.P., Ly- undup A.V., Volkov E.N., Petrakov K.V., Avramov P.V., Perov N.V., Sevastianov V.I. Correction of chronic liver failure in the transplantation of liver cells in suspension and cell engineering structures (an experimental study). Vestnik RAMSci. 2013; 4: 44–51 (in rus).
6. Шумаков В.И., Скалецкий Н.Н. Трансплантация ост- ровковых клеток поджелудочной железы. Трансплан- тология (руководство для врачей) / Под ред. акад. В.И. Шумакова. М.: МИА, 2006: 418–430.
7. Shumakov V.I., Skaletsky N.N. Transplantation of pancreatic islet cells. Transplantologiya (posobie dla vrachey). Editor acad. V.I. Shumakov. M: MIA, 2006: 418–430 (in rus).
8. Кирсанова Л.А., Баранова Н.В., Скалецкий Н.Н., Зай- денов В.А., Бубенцова Г.Н., Пушкова И.А. Поджелу- дочная железа новорожденных кроликов как источник прогениторных клеток. Вестник трансплантологии и искусственных органов. 2011; 11 (1): 61–64. Kirsanova L.A., Bubentsova G.N., Baranova N.V., Skalet- skiy N.N., Zaydenov V.A., Pushkova I.A. Newborn rabbits pancreas as a source of progenitor cells. Vestnik transplan- tologii i iskusstvennyh organov. 2011; 13 (1): 61–64 (in rus).
9. Lee S.-H., Hao E., Savinov A.Y., Geron I., Strong A.J. Itkin-Ansari P. Human B-cell precursors mature into functional insulin-producing cells in an immunoisolation device: implications for diabetes cell therapies. Trans- plantation, 2009; 87 (7): 983–991.
10. Smith R.N., Kent S.C., Nagle J., Selig M., Lafrate A.J., Najafian N. Pathology of an islet transplant 2 years after transplantation: evidence for a nonimmunological loss. Transplantation. 2008; 86 (7): 54–62.
Review
For citations:
Kirsanova L.A., Baranova N.V., Bubentsova G.N., Skaletskaya G.N., Perova N.V., Sevastianov V.I., Skaletskiy N.N. INFLUENCE OF MICROSTRUCTURED COLLAGEN HYDROGEL ON PANCREATIC ISLET CELL CULTURES. Russian Journal of Transplantology and Artificial Organs. 2014;16(1):29-33. (In Russ.) https://doi.org/10.15825/1995-1191-2014-1-29-33
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