FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO
https://doi.org/10.15825/1995-1191-2013-3-66-72
Abstract
Aim. The goal of this study was to demonstrate the possibility of in vitro formation of tissue-engineered const- ruct of cartilage on the basis of cell-engineered construct composed of biopolymer hydrogel matrix and human adipose-derived mesenchymal stromal cells. Materials and methods. As a biodegradable matrix, the hetero- geneous composition of the implantable gel Sphero®GEL (Biomir Service, Krasnoznamensk, Moscow Region) was selected. To generate cell-engineered construct of cartilaginous tissue human adipose-derived mesenchy- mal stromal cells were mixed with biopolymer matrix Sphero®GEL and cultured in chondrogenesis medium.
Results. It was revealed that human adipose-derived mesenchymal stromal cells with biopolymer matrix under chondrogenic conditions generate three-dimensional structures and produce cartilaginous extracellu- lar matrix components: collagen type II and glycosaminoglycans. Conclusion. It was discovered that 3-D structures formed in the process of culture of cell-engineered constructs, began to synthesize the components of the cartilage extracellular matrix. This indicates the initial stage of in vitro formation of tissue-engineered cartilage construct.
About the Authors
V. A. Surguchenko
Laboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian Federation
Russian Federation
Russian Federation
A. S. Ponomareva
Laboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian Federation
Russian Federation
Russian Federation
L. A. Kirsanova
Laboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician
V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Russian Federation
Russian Federation
G. N. Bubentsova
Laboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician
V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Russian Federation
Russian Federation
N. N. Skaletskij
Laboratory of cell transplantation (Head – doct. of med. sci. N.N. Skaletskiy) Academician
V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs, (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Russian Federation
Russian Federation
V. I. Sevastianov
Laboratory of biomaterials and delivery systems (Head – doct. of biol. sci., prof. V.I. Sevastianov) Academician V.I. Schumakov Federal Research Center of Transplantology and Artificial Organs (Head – academician of RAMSci, prof. S.V. Gautier), Moscow, Russian Federation
Institute of Biomedical Research and Technology (ANO) (Head – doct. of biol. sci., prof. V.I. Sevastianov), Moscow, Russian Federation
Russian Federation
Russian Federation
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Review
For citations:
Surguchenko V.A., Ponomareva A.S., Kirsanova L.A., Bubentsova G.N., Skaletskij N.N., Sevastianov V.I. FORMATION OF TISSUE-ENGINEERED CONSTRUCT OF CARTILAGE IN VITRO. Russian Journal of Transplantology and Artificial Organs. 2013;15(3):66-72. (In Russ.) https://doi.org/10.15825/1995-1191-2013-3-66-72
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