AMBIGUITY OF LOCI DURING HLA-TYPING ON SSO TECHNOLOGY AND ATTEMPT TO RESOLVE THEM
https://doi.org/10.15825/1995-1191-2010-4-33-38
Abstract
Sequence specific oligonucleotides typing was used to identify human leukocyte antigen (HLA)-A, B, DRB1 alleles from 705 recruited volunteers with Volga Federal District for unrelated hematopoietic stem cell registry and 155 of their number at locus HLA-C. 48 samples cannot be entered into the database because of ambiguities in the identification of allelic loci on HLA-class I – HLA-A, HLA-B. To resolution of ambiguity use reagents kits AlleleSEQR HLA Sequencing, which allowed to reveal the ambiguity of the locus HLA-A, 44% of cases, the loci HLA-B and HLA-C – 40% of cases. Application software HARPs Finder showed the possibility of resolution of all identified allelic ambiguities (with the exception of types of ambiguity – A*03/A*32 or A*74:13/A*32:04 and A*01/A*11 or A*36:04/A*11) with the addition of basic kits AlleleSEQR HLA Sequencing reagents kit Al- leleSEQR HARPs.
About the Authors
M. A. Loginova
Volga Federal District Medical Center of Expertise of Blood Products Quality and Investigation of Donor Plasma Fractionation
Russian Federation
Russian Federation
I. V. Paramonov
Volga Federal District Medical Center of Expertise of Blood Products Quality and Investigation of Donor Plasma Fractionation
Russian Federation
Russian Federation
N. P. Trofimova
Volga Federal District Medical Center of Expertise of Blood Products Quality and Investigation of Donor Plasma Fractionation
Russian Federation
Russian Federation
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Review
For citations:
Loginova M.A., Paramonov I.V., Trofimova N.P. AMBIGUITY OF LOCI DURING HLA-TYPING ON SSO TECHNOLOGY AND ATTEMPT TO RESOLVE THEM. Russian Journal of Transplantology and Artificial Organs. 2010;12(4):33-38. (In Russ.) https://doi.org/10.15825/1995-1191-2010-4-33-38
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