CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
https://doi.org/10.15825/1995-1191-2009-2-54-59
Abstract
We studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibroblasts maintain high proliferative ability and viability during long term cultivation. Spidroin 1-based scaffold are the perspective materials for bioengineering.
About the Authors
O. L. Pustovalova
Moscow State University, biological faculty
Russian Federation
Russian Federation
I. I. Agapov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
M. M. Moisenovich
Moscow State University, biological faculty
Russian Federation
Russian Federation
P. S. Eremin
Moscow State University, biological faculty
Russian Federation
Russian Federation
A. A. Kazulina
Moscow State University, biological faculty
Russian Federation
Russian Federation
A. Y. Arhipova
Moscow State University, biological faculty
Russian Federation
Russian Federation
A. A. Ramonova
Moscow State University, biological faculty
Russian Federation
Russian Federation
V. G. Bogush
State Scientific Center «GNIIgenetika»
Russian Federation
Russian Federation
V. I. Sevastianov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
M. P. Kirpichnikov
Moscow State University, biological faculty
Russian Federation
Russian Federation
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Review
For citations:
Pustovalova O.L., Agapov I.I., Moisenovich M.M., Eremin P.S., Kazulina A.A., Arhipova A.Y., Ramonova A.A., Bogush V.G., Sevastianov V.I., Kirpichnikov M.P. CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK. Russian Journal of Transplantology and Artificial Organs. 2009;11(2):54-59. (In Russ.) https://doi.org/10.15825/1995-1191-2009-2-54-59
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