DISTURBANCE OF THE CARDIOMYOCYTE’S MACROMOLECULAR STRUCTURE IN HEART ALLOGRAFTS AS A SIGN OF CHRONIC REJECTION
https://doi.org/10.15825/1995-1191-2012-4-17-26
Abstract
Chronic rejection, especially cardiac allograft vasculopathy, is a major limiting factor for long-term transplant survival. This process affects not only the blood vessels, but also cardiomyocytes. However, there are extremely few reports on the evaluation of their macromolecular structure state. The aim of the study was to evaluate the structural proteins of cardiomyocytes (actin, myosin, troponin I, titin, desmin, vinculin) of heart allografts in different periods after the operation (from 6 days to 15 years). Major changes of macromolecular structure were revealed in late period after transplantation (6 months – 15 years). The contribution of humoral immune response in the process of chronic cardiac allograft rejection was observed: in eight of twelve recipients episodes of acute humoral rejection had been repeatedly registered; disorders of the expression of 5 proteins out of 6 characterized were found in recipients with recurrent and persistent antibody-mediated rejection.
About the Authors
A. G. Kupriyanova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
Russian Federation
Russian Federation
L. V. Beletskaya
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
I. M. Ilyinsky
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
V. A. Zaidenov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
N. P. Mozeiko
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
R. S. Saitgareev
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
A. Y. Kormer
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
A. M. Golts
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
V. M. Zakharevich
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
S. V. Gautier
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs,
I.M. Sechenov First Moscow State Medical University, Department of transplantology and artificial organs
References
1. Белецкая Л.В, Куприянова А.Г., Ильинский И.М. и др. Молекулярно-биологическая структура кардиомио- цита // Вестник трансплантол. и искусств. органов. М., 2006. No 4. С. 89–94.
2. Белецкая Л.В., Куприянова А.Г., Кормер А.Я. и др. Ревмоподобное течение отторжения гуморального (сосудистого) типа при аллотрансплантации серд- ца // Бюллетень экспериментальной биологии и ме- дицины. 2006. Т. 142. No 9. С. 337–340.
3. Белецкая Л.В, Куприянова А.Г., Куренкова Л.Г. и др. Изменения макромолекулярной структуры кардио- миоцитов при идиопатической кардиомиопатии // Вестник трансплантол. и искусств. органов. 2007. Т. 34. No 2. С. 33–35.
4. Ильинский И.М. Патология коронарных артерий ал- лотрансплантированного сердца // Вестник транс- плантол. и искусств. органов. М., 2001. No 3–4. С. 41–46.
5. Куприянова А.Г., Белецкая Л.В., Зайденов В.А и др. Опыт использования иммуногистохимического ме- тода исследования эндомиокардиальных биопсий в диагностике отторжения гуморального типа у боль- ных с аллотрансплантатом сердца // Вестник транс- плантол. и искусств. органов. М., 2009. Т. XI. No 3. С. 30–36.
6. Куприянова А.Г., Белецкая Л.В., Зайденов В.А и др. Оценка состояния макромолекулярной структу- ры кардиомиоцитов у больных различными форма- ми кардиомиопатии // Вестник трансплантол. и ис- кусств. органов. М., 2012. Т. XIV. No 2. С. 32–42.
7. Beletskaya L.V., Kupriyanova A.G., Kormer A.Ya. Heart allograft rejection of the humoral type is a continuing «rheum-like» process // J. Heart Lung. Transplant. 2006. Vol. 25. No 8. P. 998–1000.
8. Beletskaya L.V., Kupriyanova A.G., Zaidenov V.A. et al. Altered cytoskeletal protein localization in cardiomyo- cytes of idiopathic cardiomyopathy patients // J. Heart Lung. Transplant. Aug.2007. Vol. 26. No 8. Р. 868–870.
9. Di Somma S., Di Beneditto M.P., Slavatore G. et al. Des- min-free cardiomyocytes and myocardial dysfunction in end stage heart failure // Eur. J. Heart Fail. 2004. Vol. 6. P. 389–398.
10. Franz M., Neri D., Berndt A. Chronic cardiac allograft rejection: critical role of ED-A(+) fibronectin and impli- cations for targeted therapy strategies // J. Pathol. 2012. Mar. Vol. 226 (4). Р. 557–561.
11. Hammond E.H., Yowell R.L., Price G.D. et al. Vascular rejection and its relationship to allograft coronary artery disease // J. Heart Lung. Transplant. 1992 May-Jun.
12. Vol. 11 (3 Pt 2). P. 111–119.
13. Hein S., Kostin S., Heling A. et al. The role of cytoske-
14. leton in heart failure // Cardiovascular Research. 2000.
15. Vol. 45. Р. 273–278.
16. Kobashigawa J., Crespo-Leiro M.G., Ensminger S.M.
17. et al. Report from a consensus conference on antibody- mediated rejection in heart transplantation // J. Heart Lung. Transplant. 2011 March. Vol. 30 (3). Р. 252–269.
18. Kostin S., Hein S., Arnon E., Scholz D., Schaper J. The cytoskeleton and related proteins in human failing heart // Heart Fail. Rev. 2000. Vol. 3. P. 271–280.
19. Schmauss D. and Weis M. Cardiac Allograft Vasculopa- thy: Recent Developments // Circulation. 2008. Vol. 117. Р. 2131–2141.
20. Shaper J., Froede R., Hein St. et al. Impairmant of the myocardial ultrastructure and changes of the cytoske-
21. leton in dilated cardiomyopathy // Circulation. 1991.
22. Vol. 83. No 2. P. 504–505.
23. Suzuki J-i., Isobe M., Morishita R., Nagai R. Charac-
24. teristics of Chronic Rejection in Heart Transplanta- tion: Important Elements of Pathogenesis and Future Treatments // Circulation Journal. Feb. 2010. Vol. 74. Р. 233–239.
25. Tan C.D., Baldwin W.M. 3rd, Rodriguez E.R. Update on cardiac transplantation pathology // Arch. Pathol. Lab. Med. 2007 Aug. Vol. 131 (8). P. 1169–1191.
26. Wehner J., Morrell C, Reynolds T. et al. Antibody and complement in transplant vasculopathy // Circulation Research. Feb. 2007. Vol. 100 (2). Р. 191–203.
27. Zemljic-Harpf A., Manso A.M., Ross S.R. Vinculin and Talin: Focus on the Myocardium. J. Investig Med. 2009. Vol. 57 (8). Р. 849–855.
Review
For citations:
Kupriyanova A.G., Beletskaya L.V., Ilyinsky I.M., Zaidenov V.A., Mozeiko N.P., Saitgareev R.S., Kormer A.Y., Golts A.M., Zakharevich V.M., Gautier S.V. DISTURBANCE OF THE CARDIOMYOCYTE’S MACROMOLECULAR STRUCTURE IN HEART ALLOGRAFTS AS A SIGN OF CHRONIC REJECTION. Russian Journal of Transplantology and Artificial Organs. 2012;14(4):17-26. (In Russ.) https://doi.org/10.15825/1995-1191-2012-4-17-26
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