Technique for decellularization of corneal lenticules for subsequent keratoplasty

Abstract

This article addresses strategies for reducing corneal graft rejection and explores alternatives to full-thickness corneal transplantation. The objective of the study is to develop a technique for removing cellular material from human corneal lenticules obtained during refractive surgery for use as a biomaterial in keratoplasty. A decellularization procedure was developed in which lenticules were immersed in a 1% aqueous solution of sodium lauryl sulfate for 24 hours, followed by detergent removal using a buffered 0.9% NaCl solution for 5 days. Decellularization effectiveness was confirmed by the absence of genetic material using Hoechst staining and hematoxylin & eosin staining. Histological analysis of the biomaterial after preservation for various periods demonstrated no signs of biodegradation of the acellular lenticules, which remained sterile for at least 12 weeks. A comparative experimental study in rabbits demonstrated the efficacy and safety of the proposed technique.