LONG-TERM (OF MANY DAYS) NORMOTHERMAL LIMBAL GRAFTS PRESERVATION AS A METHOD OF QUANTITY AND ACTIVITY INCREASE OF MMSC-LIKE LIMBAL CELLS
https://doi.org/10.15825/1995-1191-2012-2-77-85
Abstract
The reliability increase of corneal transplants engraftment may be achieved by combined transplantation with preserved limbal grafts (LG). We studied two methods of LG preservation: normothermal preservation in Borzenok–Moroz medium or standard culture medium for 35 days and cryopreservation at –80 °C in Borzenok– Moroz medium with/without DMSO addition for 1 month. We found that long-term normothermal preservation (for 28 days) and cryopreservation maintain LG cells viability and their capacity to maintain cytokine balance as well as to keep cytokine balance and to retain phenotype of MMSC (multipotent mesenchymal stromal cells) and to activate tolerogenic properties of limbal cells the latter improving quality and increasing capacity of corneal transplant engraftment reliability without systemic immunosupression.
About the Authors
S. A. Borzenok
The S. Fyodorov Eye Microsurgery Complex FSBI
N. A. Onischenko
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Kh. D. Tonaeva
The S. Fyodorov Eye Microsurgery Complex FSBI
Y. A. Komakh
The S. Fyodorov Eye Microsurgery Complex FSBI
V. S. Suskova
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
S. I. Suskov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
L. V. Didenko
Gamaleya Research Institut for Epidemiology and Microbiology
N. V. Shevlyagina
Gamaleya Research Institut for Epidemiology and Microbiology
E. A. Kost
Gamaleya Research Institut for Epidemiology and Microbiology
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Review
For citations:
Borzenok S.A., Onischenko N.A., Tonaeva Kh.D., Komakh Y.A., Suskova V.S., Suskov S.I., Didenko L.V., Shevlyagina N.V., Kost E.A. LONG-TERM (OF MANY DAYS) NORMOTHERMAL LIMBAL GRAFTS PRESERVATION AS A METHOD OF QUANTITY AND ACTIVITY INCREASE OF MMSC-LIKE LIMBAL CELLS. Russian Journal of Transplantology and Artificial Organs. 2012;14(2):77-85. (In Russ.) https://doi.org/10.15825/1995-1191-2012-2-77-85
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