CHARACTERISTICS OF ADHESION AND PROLIFERATION OF MOUSE NIH/3T3 FIBROBLASTS ON THE POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) FILMS WITH DIFFERENT SURFACE ROUGHNESS VALUES
https://doi.org/10.15825/1995-1191-2012-1-72-77
Abstract
Adhesion and proliferation of NIH/3Т3 mouse fibroblasts on the surfaces of bacterial copolymer poly(3-hydroxy- butyrate-co-3-hydroxyvalerate) films with different roughness was investigated. Atomic force microscopic analysis showed that surface roughness values of all films were significantly greater (92.0 ± 7.0; 290.8 ± 7.0; 588.8 ± 16.0 nm) than that of the cultural plastic control (9.5 ±0.6 nm). It was revealed that adhesion and metabolic activity of the cells decreases with the increase of surface roughness values. NIH/3Т3 mouse fibroblasts attached weakly to the surface of copolymer films and washed away the substrate during rinsing and staining.
About the Authors
V.А. Surguchenko
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
А.S. Ponomareva
АNO «Institute of Biomedical Research and Technology», Moscow
Russian Federation
Russian Federation
А.Е. Efimov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
Е.А. Nemets
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
I.I. Agapov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
V.I. Sevastianov
Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, Moscow
Russian Federation
Russian Federation
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Review
For citations:
Surguchenko V., Ponomareva А., Efimov А., Nemets Е., Agapov I., Sevastianov V. CHARACTERISTICS OF ADHESION AND PROLIFERATION OF MOUSE NIH/3T3 FIBROBLASTS ON THE POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE) FILMS WITH DIFFERENT SURFACE ROUGHNESS VALUES. Russian Journal of Transplantology and Artificial Organs. 2012;14(1):72-77. (In Russ.) https://doi.org/10.15825/1995-1191-2012-1-72-77
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