Protocol for assessing the effectiveness of lung preservation solutions in donation after circulatory death donors

Objective: to evaluate the effectiveness of lung preservation in donation after circulatory death (DCD) using a non-perfusion-based preservation method. Materials and methods. The study was conducted on eight healthy male Landrace pigs (weight range: 40–60 kg), divided into three groups based on the preservation solution used: Celsior group (n = 3), lungs preserved using a solution prepared according to the Celsior formulation.; NewSolution group (n = 3), lungs preserved using a custom-formulated solution developed in-house; NaCl group (n = 2), lungs preserved using saline (control group). Results. In the study groups (Celsior and NewSolution), the oxygenation index after reperfusion exceeded 350, while the control group (NaCl) exhibited an oxygenation index of less than 350. Conclusions. The method described for evaluating the effectiveness of new lung preservation solutions is technically simple and cost-effective, as it enables rapid experimentation with a sufficiently large number of observations. While this approach may not address all challenges in experimental transplantology, it provides a practical and efficient tool for preliminary screening of lung preservation strategies.

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