vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2018-4-69-75vtio-952Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesАнализ пролиферативной активности клеток в микрочастицах, полученных из децеллюляризованной ткани печени и почкиThe analysis of the proliferative activity of cells on microparticles obtained from decellularized liver and kidney tissueБоброваМ. М.BobrovaM. M.

Лаборатория бионанотехнологий; Биологический факультет МГУ им. М.В. Ломоносова

Москва

Laboratory of Bionanotechnology, V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation; Biological faculty, Lomonosov Moscow State University

Moscow

СафоноваЛ. А.SafonovaL. A.

Лаборатория бионанотехнологий; Биологический факультет МГУ им. М.В. Ломоносова

Москва

Laboratory of Bionanotechnology, V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation; Biological faculty, Lomonosov Moscow State University

Moscow

АгаповаО. И.AgapovaO. I.

Лаборатория бионанотехнологий

Москва

Laboratory of Bionanotechnology

Moscow

ЕфимовА. Е.EfimovA. E.

Лаборатория бионанотехнологий

Москва

Laboratory of Bionanotechnology

Moscow

АгаповИ. И.AgapovI. I.

Лаборатория бионанотехнологий

123182, Москва, ул. Щукинская, д. 1Тел. (985) 231-60-42

Laboratory of Bionanotechnology

1, Shchukinskaya str., Moscow, 123182.Теl. (985) 231-60-42.

igor_agapov@mail.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России; ФГБОУ ВО «Московский государственный университет имени М.В. Ломоносова»РоссияV.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian Federation; Lomonosov Moscow State UniversityRussian FederationФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияV.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs of the Ministry of Healthcare of the Russian FederationRussian Federation2018300120192046975Copyright © Боброва М.М., Сафонова Л.А., Агапова О.И., Ефимов А.Е., Агапов И.И., 20192019Боброва М.М., Сафонова Л.А., Агапова О.И., Ефимов А.Е., Агапов И.И.Bobrova M.M., Safonova L.A., Agapova O.I., Efimov A.E., Agapov I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/952Цель

Цель. Разработка протоколов децеллюляризации ткани печени и почек, а также анализ пролиферативной активности клеток гепатокарциномы человека Hep-G2 на различных носителях.

Материалы и методы

Материалы и методы. Децеллюляризация печени и почек была произведена путем перфузии растворов детергентов с постепенно возрастающими концентрациями Тритона Х-100 (1, 2 и 3%). Был произведен гистологический анализ полученных образцов, использован метод оптической и сканирующей электронной микроскопии для исследования полученных образцов. Исследована пролиферативная активность клеток гепатокарциномы человека Hep-G2 на полученных образцах децеллюляризованной ткани печени и почки.

Результаты

Результаты. Децеллюляризация органа не приводит к изменениям в специфической структуре матрикса ткани. Микрочастицы со средним размером 200 мкм были изготовлены из децеллюляризованного матрикса тканей печени и почки. Уровень пролиферативной активности клеток гепатокарциномы человека Hep-G2 , культивированных на микрочастицах из децеллюляризованной печени, был существенно выше, чем на микрочастицах из децеллюляризованной почки.

Заключение

Заключение. Децеллюляризованный матрикс сохраняет нативную трехмерную структуру ткани. Уровень пролиферативной активности клеток существенно выше на микрочастицах из децеллюляризованной печени, что подтверждает сохранность специфичности межклеточного матрикса ткани после процесса децеллюляризации.

Aim

Aim. To develop the protocols for liver and kidney tissue decellularization, and to develop an analysis of the proliferative activity of human Hep-G2 hepatocarcinoma cells on various carriers.

Materials and methods

Materials and methods. Decellularization of the liver and kidneys was performed by perfusion of detergent solutions with gradually increasing concentrations of Triton X-100 (1, 2 and 3%). A histological analysis of the obtained samples was performed, and the method of optical and scanning electron microscopy was used to study the obtained samples. The proliferative activity of human Hep-G2 hepatocarcinoma cells was studied on the obtained samples of decellularized liver and kidney tissue.

Results

Results. Decellularization of the organ does not lead to changes in the specific structure of the tissue matrix. Microparticles with an average size of 200 μm were made from their decellularized matrix of liver and kidney tissues. The level of proliferative activity of human Hep-G2 hepatocarcinoma cells cultured on microparticles from a decellularized liver was significantly higher than on microparticles from a decellularized kidney.

Conclusion

Conclusion. The decellularized matrix retains the native three-dimensional structure of the tissue. The level of cell proliferative activity is significantly higher on microparticles from the decellularized liver, which confirms the preservation of the specificity of the extracellular matrix of the tissue after the process of decellularization.

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The authors declare that there are no conflicts of interest present.