vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2018-1-70-78vtio-857Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesКУЛЬТИВИРОВАНИЕ КЛЕТОК ПЕЧЕНИ ЧЕЛОВЕКА И МЕЗЕНХИМАЛЬНЫХ СТРОМАЛЬНЫХ КЛЕТОК ЖИРОВОЙ ТКАНИ ЧЕЛОВЕКА В ПЕРФУЗИОННОМ БИОРЕАКТОРЕCULTIVATION OF HUMAN LIVER CELLS AND ADIPOSE-DERIVED MESENCHYMAL STROMAL CELLS IN PERFUSION BIOREACTORБасокЮ. Б.BasokYu. В.

Басок Юлия Борисовна

123182, Москва, ул. Щукинская, д. 1

Basok Yulia Borisovna

1, Shchukinskaya st., Moscow, 123182

bjb2005@mail.ruГригорьевА. М.GrigoryevA. M.КирсановаЛ. А.KirsanovaL. A.ШмеркоН. П.ShmerkoN. P.ХизроевХ. М.KhizroevК. M.СевастьяновВ. И.SevastianovV. I.ФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова»РоссияV.I. Shumakov National Medical Research Center of Transplantology and Artifi cial Organs of the Ministry of Healthcare of the Russian FederationRussian Federation2018240420182017078Copyright © Басок Ю.Б., Григорьев А.М., Кирсанова Л.А., Шмерко Н.П., Хизроев Х.М., Севастьянов В.И., 20182018Басок Ю.Б., Григорьев А.М., Кирсанова Л.А., Шмерко Н.П., Хизроев Х.М., Севастьянов В.И.Basok Y.В., Grigoryev A.M., Kirsanova L.A., Shmerko N.P., Khizroev К.M., Sevastianov V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/857Цель

Цель. Культивирование клеток печени человека и мезенхимальных стромальных клеток жировой ткани человека в перфузионном биореакторе.

Материалы и методы

Материалы и методы. В перфузионном биореакторе проведены эксперименты по культивированию клеточно-инженерной конструкции (КИК), состоящей из биополимерного микроструктурированного коллагенсодержащего гидрогеля (БМКГ), клеток печени человека, мезенхимальных стромальных клеток жировой ткани человека (МСК ЖТч) и культуральной среды William’s E.

 Результаты

 Результаты. В ходе эксперимента установлено, что на 7-е сутки в культуральных камерах биореактора присутствовали крупные клетки с характерной для гепатоцитов морфологией – полигональной формой и центрально расположенным круглым ядром. Метаболическая активность гепатоцитов в исследуемой КИК подтверждалась присутствием мочевины в культуральной среде на седьмые сутки культивирования в биореакторе и резорбцией БМКГ.

Aim

Aim: to show the progress of the experiment of cultivation of human liver cells and adipose-derived mesenchymal stromal cells in perfusion bioreactor.

Materials and methods

Materials and methods. The cultivation of a cell-engineered construct, consisting of a biopolymer microstructured collagen-containing hydrogel, human liver cells, adipose-derived mesenchymal stromal cells, and William’s E Medium, was performed in a perfusion bioreactor.

Results

Results. On the 7th day large cells with hepatocyte morphology – of a polygonal shape and a centrally located round nucleus, – were present in the culture chambers of the bioreactor. The metabolic activity of hepatocytes in cell-engineered constructs was confi rmed by the presence of urea in the culture medium on the seventh day of cultivation in the bioreactor and by the resorption of a biopolymer microstructured collagen-containing hydrogel.

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The authors declare that there are no conflicts of interest present.