vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2015-1-68-73vtio-498Research ArticleКлиническая трансплантологияClinical TransplantologyОСОБЕННОСТИ СУБПОПУЛЯЦИОННОГО СОСТАВА ЛИМФОЦИТОВ КРОВИ У РЕЦИПИЕНТОВ В ТЕЧЕНИЕ ПЕРВОГО МЕСЯЦА ПОСЛЕ ПЕРЕСАДКИ ПЕЧЕНИFEATURES OF SUBPOPULATION COMPOSITION OF BLOOD LYMPHOCYTES IN RECIPIENTS WITHIN THE FIRST MONTH AFTER LIVER TRANSPLANTATIONШуткоА. Н.ShoutkoA. N.shoutko@inbox.ruГерасимоваО. А.GerasimovaO. A.ЕкимоваЛ. П.EkimovaL. P.ЖеребцовФ. К.ZherebtsovF. K.ГрановА. М.GranovA. M.Российский научный центр радиологии и хирургических технологий Минздрава РФ, Санкт-Петербург, Российская ФедерацияРоссияRussian Scientific Center for Radiology and Surgical Technologies of the Ministry of Healthcare of the Russian Federation, St-Petersburg, Russian FederationRussian Federation2015030420151716873Copyright © Шутко А.Н., Герасимова О.А., Екимова Л.П., Жеребцов Ф.К., Гранов А.М., 20152015Шутко А.Н., Герасимова О.А., Екимова Л.П., Жеребцов Ф.К., Гранов А.М.Shoutko A.N., Gerasimova O.A., Ekimova L.P., Zherebtsov F.K., Granov A.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/498

Соотношения субпопуляционного состава мононуклеарных клеток крови у 68 пациентов исследованы методом проточной цитометрии до и в течение первого месяца после трансплантации трупной печени с целью определения их возможного вклада в процессы приживления трансплантата. Полученные данные позволяют рассматривать изменения регуляторных Т-клеток после трансплантации как частный случай генерализованного смещения всего дифференцировочного процесса в лимфоцитопоэзе, начиная со стволовых гемопоэтических клеток и прелимфоцитов. Смещение сопровождается увеличением юных гематопоэтических стволовых клеток, клеток предшественников лимфоцитарного ряда, клеток с ангиогенными свойствами и уменьшением большинства более зрелых дифференцированных форм лимфоцитов. Ослабление «толерогенной» активности печени у больных в листе ожидания трансплантации печени и восстановление ее после трансплантации объяснено морфообразующим, трофическим механизмом. Основу этого механизма составляет увеличение в крови стволовых гемопоэтических клеток и ангиогенных клеток, переносящих регенераторную информацию к трансплантату. 

Ratios of subpopulations of mononuclear blood cells in 68 patients were registered by method of flow cytometry during one month before or after transplantation of a cadaveric liver with the aim of a comparative assessment of the contribution of cellular factors into graft acceptance. Results. The obtained data allow considering the changes of separate Treg subpopulation after transplantation as a partial outcome of generalized shift of the whole process of lymphocytes differentiation, involving hematopoietic stem cells and prelymphocytes. This shift is followed by an increase of young hematopoietic stem cells, precursors of lymphocytes, angiogenic cells, and by a concomitant reduction of the majority of more matured subpopulations of lymphocytes. Conclusion. Diminishment of «tolerogenic» liver activity of before transplantation and its restoration after the organ’s replacement is explained by morphogenic/trophic mechanism. The basis of this mechanism is an increase in blood of hematopoietic stem cells and other cells transferring angiogenic and regenerative information to the graft. 

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The authors declare that there are no conflicts of interest present.