vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2012-4-74-79vtio-220Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesОСОБЕННОСТИ ФОРМИРОВАНИЯ ОСТРОВКОВОПОДОБНЫХ КЛАСТЕРОВ ПРИ КУЛЬТИВИРОВАНИИ МОНОСЛОЯ ПРОТОКОВОГО ЭПИТЕЛИЯFEATURES OF ISLET-LIKE CLUSTERS GENERATION IN PANCREATIC DUCTAL CELL MOLOLAYER CULTURINGКирсановаЛ. А.KirsanovaL. A.БубенцоваГ. Н.BubentsovaG. N.БарановаН. В.BaranovaN. V.СкалецкийН. Н.SkaletskiyN. N.ЗайденовВ. А.ZaydenovV. A.ПушковаИ. А.PushkovaI. A.ФГБУ «ФНЦ трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России, г. МоскваРоссияAcademician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, MoscowRussian Federation201229052014144ВЕСТНИК ТРАНСПЛАНТОЛОГИИ И ИСКУССТВЕННЫХ ОРГАНОВ том XIV No 4–20127479Copyright © Кирсанова Л.А., Бубенцова Г.Н., Баранова Н.В., Скалецкий Н.Н., Зайденов В.А., Пушкова И.А., 20122012Кирсанова Л.А., Бубенцова Г.Н., Баранова Н.В., Скалецкий Н.Н., Зайденов В.А., Пушкова И.А.Kirsanova L.A., Bubentsova G.N., Baranova N.V., Skaletskiy N.N., Zaydenov V.A., Pushkova I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/220

Монослой протоковых клеток поджелудочной железы новорожденного кролика при культивировании в бессывороточной среде структурно видоизменялся, формируя по истечении двухнедельного срока трех- мерные островковоподобные кластеры. Основные типы островковых клеток (β- и α-клетки), ранее не вы- являемые в однослойных культурах, идентифицировались в островковоподобных кластерах методом им- муногистохимии. Происхождение этих эндокринных клеток, их вероятные предшественники являются предметом обсуждения. 

Newborn rabbit pancreatic cell monolayer was obtained as we described earlier.The cultivated epithelial cells were shown by immunofluorescence to express special ductal marker CK19 and were insulin-and glucagon- negative for 10–15 days. A few fusiforms of nestin-positive cells were found in monolayer. Over 2 weeks in serum-free medium the plaques of epithelial cells became crowded and formed 3-dimentional structures – islet- like clusters. Islet-like clusters contain some insulin- and glucagon-positive cells recognized by immunohysto- chemistry staining. Pancreatic endocrine cell generation in 3-dimentional structures is discussed. 

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The authors declare that there are no conflicts of interest present.