vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2026-2-227-237vtio-2070Research ArticleЭКСПЕРИМЕНТАЛЬНЫЕ ИССЛЕДОВАНИЯEXPERIMENTAL RESEARCHРезультаты экспериментального применения аллогенных и ксеногенных биоматериалов для регенерации циркулярного дефекта кишкиExperimental evaluation of allogeneic and xenogeneic biomaterials for regeneration of circular intestinal defectshttps://orcid.org/0000-0001-6578-8909НасибуллинИ. М.NasibullinI. M.

Насибуллин Ильдар Марсович.

450008, Уфа, ул. Ленина, 3

Тел. (905) 000-83-76

Ildar M. Nasibullin.

3, Lenina str., Ufa, 450008

Phone: (905) 000-83-76

nim_76@mail.ruhttps://orcid.org/0000-0002-3681-2751ХасановР. Р.KhasanovR. R.

Хасанов Расуль Ринатович.

Уфа, Российская Федерация; Гайдельберг, Федеративная Республика Германия

Ufa, Russian Federation; Heidelberg, Germany

Rasul222@mail.ruhttps://orcid.org/0000-0003-1686-1254ЛебедеваА. И.LebedevaA. I.

Лебедева Анна Ивановна.

Уфа

Ufa

jeol02@mail.ruШангинаО. Р.ShanginaO. R.

Уфа

Ufa

https://orcid.org/0000-0003-2125-4897ПавловВ. Н.PavlovV. N.

Павлов Валентин Николаевич.

Уфа

Ufa

pavlov@bashgmu.ruhttps://orcid.org/0009-0000-2946-7710ХалиловД. И.KhalilovD. E.

Халилов Данил Ильмирович.

Уфа

Ufa

halilovdanil2001@yandex.ruФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава РоссииРоссияBashkir State Medical UniversityRussian FederationФГБОУ ВО «Башкирский государственный медицинский университет» Минздрава России; Клиника детской хирургии медицинского факультета Мангейм Университета ГайдельбергГерманияBashkir State Medical University; Heidelberg UniversityGermany202628062026282227237Copyright © Насибуллин И.М., Хасанов Р.Р., Лебедева А.И., Шангина О.Р., Павлов В.Н., Халилов Д.И., 20262026Насибуллин И.М., Хасанов Р.Р., Лебедева А.И., Шангина О.Р., Павлов В.Н., Халилов Д.И.Nasibullin I.M., Khasanov R.R., Lebedeva A.I., Shangina O.R., Pavlov V.N., Khalilov D.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/2070

Целью работы явилось исследование регенераторных свойств аллогенных и ксеногенных биоматериалов для восстановления циркулярных дефектов кишки. Материалы и методы. В эксперименте использовали крыс-самцов линии Wistar половозрелых, возраст 1 год, вес 300 г (n = 98). В опытной группе животным (n = 49) трансплантировали аллогенный децеллюляризированный биоматериал, изготовленный из кишки крысы. В контрольной группе (n = 49) пересаживали ксеногенный биоматериал, изготовленный из кадаверной фиброзной капсулы почки человека. У крыс обеих групп была резецирована часть подвздошной кишки размером 30 мм, после чего в область дефекта помещали биоматериалы в виде трубки аналогичных размеров конец в конец. В последующем исследуемые биоматериалы подвергались децеллюляризации и морфологическим исследованиям. Результаты. В опытной группе через 2–3 месяца после трансплантации аллогенного децеллюляризированного биоматериала восстанавливалась архитектоника всех слоев стенки тонкой кишки, аллогенный биоматериал полностью резорбировался и замещался органоспецифическим регенератом. Верифицировано наличие кровеносных сосудов, нервных волокон и пейеровых бляшек. В контрольной группе после трансплантации ксеногенного биоматериала определялась смертность животных в 100% случаях в ранние сроки (7–14 сут) в связи с отторжением трансплантата и развитием выраженной иммунной воспалительной реакции в брюшной полости. Заключение. Использование предложенного метода в эксперименте достоверно способствует регенерации протяженного участка тонкой кишки от 30 мм, демонстрирует возможность восстановления кишечника со всеми структурными элементами, присущими кишечной стенке, и может быть применимо для лечения заболеваний, ассоциированных с СКК, воспалительно-деструктивными заболеваниями или для восполнения циркулярного дефекта после резекции кишки.

Objective: to investigate the regenerative potential of allogeneic and xenogeneic biomaterials in the repair of circular bowel defects. Materials and methods. The study was conducted on sexually mature male Wistar rats (1 year old, weighing ~300 g; n = 98). In the experimental group (n = 49), allogeneic decellularized biomaterial (ADB) derived from rat bowel was implanted. In the control group (n = 49), xenogeneic biomaterial derived from the fibrous capsule of a human cadaveric kidney was used. In both groups, a 30-mm segment of the ileum was resected, and the biomaterials were implanted at the defect site as end-to-end tubular grafts of similar dimensions. The implanted biomaterials were subsequently subjected to decellularization and morphological analysis. Results. In the experimental group, 2–3 months after transplantation of ADB, the full architectural structure of the small bowel wall was restored. The implanted biomaterial was completely resorbed and replaced by an organ-specific regenerate, with confirmed presence of blood vessels, nerve fibers, and Peyer’s patches. In the control group, transplantation of xenogeneic biomaterial resulted in 100% mortality within the early postoperative period (7–14 days), associated with graft rejection and a pronounced immune-inflammatory response in the abdominal cavity. Conclusion. The proposed approach promotes regeneration of continuous small bowel segments measuring 30 mm or more and enables restoration of all structural components of the bowel wall. This method shows potential for the treatment of conditions such as short bowel syndrome, inflammatory-destructive bowel diseases, and for the closure of circular defects following bowel resection.

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The authors declare that there are no conflicts of interest present.