vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2026-2-83-93vtio-2059Research ArticleРЕГЕНЕРАТИВНАЯ МЕДИЦИНА И КЛЕТОЧНЫЕ ТЕХНОЛОГИИREGENERATIVE MEDICINE AND CELL TECHNOLOGIESАнализ особенностей наноструктуры миоцитов скелетной мышечной ткани методом сканирующей зондовой нанотомографииNanostructural organization of skeletal muscle myocytes revealed by scanning probe nanotomographyhttps://orcid.org/0000-0002-0769-301XЕфимовА. Е.EfimovA. E.

Ефимов Антон Евгеньевич.

Москва

Anton E. Efimov.

Moscow

antefimov@gmail.comМиленинТ. К.MileninT. K.

Миленин Тимофей Кузьмич.

Москва

Timofey K. Milenin.

Moscow

milenin.tk@phystech.eduhttps://orcid.org/0009-0002-7064-2570ПодболотоваЕ. И.PodbolotovaE. I.

Подболотова Екатерина Игоревна.

Москва

Ekaterina I. Podbolotova.

Moscow

podbolotova.ei@phystech.eduhttps://orcid.org/0000-0003-1100-9513ГрудининН. В.GrudininN. V.

Грудинин Никита Владимирович.

Москва

Nikita V. Grudinin.

Moscow

zbignev.religa@mail.ruhttps://orcid.org/0000-0002-4507-1852АгаповаО. И.AgapovaO. I.

Агапова Ольга Игоревна.

Москва

Olga I. Agapova.

Moscow

olya.agape@gmail.comhttps://orcid.org/0000-0002-0273-4601АгаповИ. И.AgapovI. I.

Агапов Игорь Иванович.

123182, Москва, ул. Щукинская, д. 1

Тел. (499) 190-66-19

Igor I. Agapov.

1, Shchukinskaya str., Moscow, 123182

Phone: (499) 190-66-19

igor_agapov@mail.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian FederationФГАОУ ВО «Московский физико-технический институт (Национальный исследовательский университет)»РоссияMoscow Institute of Physics and TechnologyRussian Federation2026280620262828393Copyright © Ефимов А.Е., Миленин Т.К., Подболотова Е.И., Грудинин Н.В., Агапова О.И., Агапов И.И., 20262026Ефимов А.Е., Миленин Т.К., Подболотова Е.И., Грудинин Н.В., Агапова О.И., Агапов И.И.Efimov A.E., Milenin T.K., Podbolotova E.I., Grudinin N.V., Agapova O.I., Agapov I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/2059

Цель: провести статистический анализ угловых ориентаций гексагональных решеток миофиламентов в миофибриллах скелетных мышц крысы с использованием технологии сканирующей зондовой нанотомографии. Материалы и методы. Для исследования были получены образцы скелетных мышечных тканей здоровых крыс породы Wistar. Препараты мышечных волокон поясничной мышцы крысы были залиты в эпоксидную смолу. Исследование поверхности образцов после среза ультрамикротомом производилось методом сканирующей зондовой нанотомографии. Анализ изображений срезов саркомеров позволяет определить ориентации гексагональных решеток миозиновых миофиламентов. Результаты. Разработана методика вычисления угловых ориентаций гексагональных решеток по муаровым структурам на изображениях срезов саркомеров. Статистический анализ полученных изображений показал, что относительные повороты гексагональных решеток не подчиняются нормальному распределению по тесту Колмогорова–Смирнова (p = 0,3) и решетки меняют свою ориентацию вдоль миофибрилл случайным образом. Заключение. Анализ ориентаций решеток в смежных саркомерах позволил впервые в научной практике получить и статистически проанализировать данные об их взаимных ориентациях на протяжении миофибрилл. По результатам расчетов выявлена низкая степень согласованности ориентаций миозиновых решеток вдоль миофибрилл в скелетных мышцах крысы. Разработанные подходы и алгоритмы могут в дальнейшем быть применены для исследований особенностей наноструктурной организации миоцитов и кардиомиоцитов различных видов.

Objective: to perform a statistical analysis of the angular orientations of hexagonal myofilament lattices in rat skeletal muscle myofibrils using scanning probe nanotomography (SPN). Materials and methods. Skeletal muscle tissue samples were obtained from healthy Wistar rats for the study. Specimens of rat lumbar skeletal muscle fibers were embedded in epoxy resin. After sectioning with an ultramicrotome, the specimen surfaces were examined using SPN. Analysis of the resulting images of sarcomere cross-sections enabled the determination of the angular orientations of the hexagonal lattices formed by myosin myofilaments. Results. A method was developed to calculate the angular orientations of hexagonal lattices based on moiré patterns observed in images of sarcomere cross-sections. Statistical analysis of the obtained images showed that the relative rotations of the hexagonal lattices do not follow a normal distribution according to the Kolmogorov–Smirnov test (p = 0.3). The results indicate that the orientations of the lattices vary randomly along the myofibrils. Conclusion. Analysis of lattice orientations in adjacent sarcomeres made it possible, for the first time, to obtain and statistically evaluate data on their mutual orientations along myofibrils. The results revealed a low degree of alignment in the orientations of myosin filaments within myofibrils of rat skeletal muscle. The proposed methods and algorithms may be further applied to studies of the nanostructural organization of myocytes and cardiomyocytes in different species.

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The authors declare that there are no conflicts of interest present.