vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2025-2-139-147vtio-1956Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesВлияние имплантации клеточно-инженерной конструкции поджелудочной железы на островковый аппарат крыс-реципиентов с сахарным диабетом I типаImpact of pancreatic cell-engineered constructs on the islet apparatus in recipient rats with type I diabetes mellitushttps://orcid.org/0000-0003-4022-0772БарановаН. В.BaranovaN. V.

Баранова Наталья Владимировна, старший научный сотрудник отдела биомедицинских технологий и тканевой инженерии

123182, Москва, ул. Щукинская, д. 1

Natalia V. Baranova

1, Shchukinskaya str., Moscow, 123182

barnats@yandex.ruКирсановаЛ. А.KirsanovaL. A.

Кирсанова Людмила Анфилофьевна, старший научный сотрудник отдела биомедицинских технологий и тканевой инженерии

Москва

Moscow

lyudochkakirsanova@mail.ruБубенцоваГ. Н.BubentsovaG. N.

Бубенцова Галина Николаевна, научный сотрудник отдела биомедицинских технологий и тканевой инженерии

Москва

Moscow

galina.bubentsova60@gmail.comПономареваА. С.PonomarevaA. S.

Пономарева Анна Сергеевна, ведущий научный сотрудник отдела биомедицинских технологий и тканевой инженерии

Москва

Moscow

a.s.ponomareva@gmail.comНикольскаяА. О.NikolskayaA. O.

Никольская Алла Олеговна, лаборант-исследователь отдела биомедицинских технологий и тканевой инженерии

Москва

Moscow

allanik64@yandex.ruБасокЮ. Б.BasokYu. B.

Басок Юлия Борисовна, заведующая отделом биомедицинских технологий и тканевой инженерии

Москва

Moscow

bjb2005@mail.ruСевастьяновВ. И.SevastianovV. I.

Севастьянов Виктор Иванович 

Москва

 

Moscow

viksev@yandex.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian FederationФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава России; АНО «Институт медико-биологических исследований и технологий»,РоссияShumakov National Medical Research Center of Transplantology and Artificial Organs; Institute of Biomedical Research and TechnologyRussian Federation202512072025272139147Copyright © Баранова Н.В., Кирсанова Л.А., Бубенцова Г.Н., Пономарева А.С., Никольская А.О., Басок Ю.Б., Севастьянов В.И., 20252025Баранова Н.В., Кирсанова Л.А., Бубенцова Г.Н., Пономарева А.С., Никольская А.О., Басок Ю.Б., Севастьянов В.И.Baranova N.V., Kirsanova L.A., Bubentsova G.N., Ponomareva A.S., Nikolskaya A.O., Basok Y.B., Sevastianov V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1956

Современные исследования направлены на изучение методов стимулирования регенераторной способ- ности β-клеток поджелудочной железы (ПЖ) как возможного терапевтического средства при сахарном диабете.

Цель

Цель: провести сравнительный анализ гистологической картины островкового аппарата у крыс со стрептозотоциновым сахарным диабетом после введения клеточно-инженерной конструкции поджелу- дочной железы (КИК ПЖ) на основе изолированных аллогенных островков Лангерганса и скаффолда из децеллюляризованных фрагментов ПЖ человека.

Материалы и методы

Материалы и методы. ПЖ крыс контрольной группы (n = 4; СД I без лечения), опытной группы 1 (n = 4; внутрибрюшинное введение островков Лангерганса) и опытной группы 2 (n = 4; внутрибрюшинное введение КИК ПЖ) подвергали гистологическому исследова- нию. Выполняли иммуногистохимическое окрашивание на инсулин и глюкагон с использованием антител и системы визуализации.

Результаты

Результаты. В островках ПЖ крыс контрольной группы иммунопозитивные клетки к инсулину не выявлялись или обнаруживались одиночными, при этом α-клетки становились пре- валирующим клеточным типом. В ПЖ крыс опытной группы 1 в большинстве островков и окружающей экзокринной паренхиме β-клетки наблюдались в количестве 1–2 в поле зрения; α-клетки продолжали оставаться основной клеточной популяцией. В ПЖ крыс опытной группы 2 определялось значительное увеличение инсулинпозитивных клеток, при этом отмечалось снижение количества глюкагонпозитивных клеток.

Заключение

Заключение. Морфологическое исследование островкового аппарата ПЖ экспериментальных животных показало, что имплантация КИК ПЖ положительно влияла на восстановление пула активно функционирующих β-клеток реципиента, выполняя роль триггера регенеративного процесса.

Current research focuses on exploring strategies to stimulate the regenerative capacity of pancreatic beta cells as a potential therapeutic approach for diabetes mellitus (DM).

Objective

Objective: this study aims to perform a comparative histological analysis of the islet apparatus in rats with streptozotocin (STZ)-induced DM following the implantation  of a pancreatic cell-engineered construct (PCEC). The PCEC consists of isolated allogeneic islets of Langerhans embedded within a scaffold derived from decellularized human pancreatic fragments.

Materials and methods

Materials and methods. The pancreases of rats from the control group (n = 4; untreated type 1 DM – T1DM), experimental group 1 (n = 4; intraperitoneal injection of pancreatic islets), and experimental group 2 (n = 4; intraperitoneal injection of PCEC) underwent histological analysis. Immunohistochemical staining for insulin and glucagon was performed using specific antibodies and an imaging system.

Results

Results. In the pancreatic islets of the control group, insulin- immunopositive beta cells were either absent or detected as isolated cells, with alpha cells predominating. In the pancreases of experimental group 1 rats, beta cells were observed in most islets and within the surrounding exocrine parenchyma, albeit in low numbers (1–2 per field of view), while alpha cells remained the dominant population. A significant increase in insulin-positive cells was observed in the pancreas of rats in experimental group 2, along with a reduction in glucagon-positive cell numbers.

Conclusion

Conclusion. Morphological examination of the pancreatic islet apparatus in the experimental animals revealed that implantation of the PCEC had a be- neficial effect on restoration of the recipient’s pool of functionally active beta cells, serving as a trigger for the regenerative process.

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The authors declare that there are no conflicts of interest present.