vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2009-2-29-36vtio-179Research ArticleКлиническая трансплантологияClinical TransplantologyМОРФОЛОГИЧЕСКИЕ И ИММУНОГИСТОХИМИЧЕСКИЕ ПРОЯВЛЕНИЯ РАЗЛИЧНЫХ ФОРМ ОСТРОГО ОТТОРЖЕНИЯ ПОЧЕЧНОГО АЛЛОТРАНСПЛАНТАТАMORPHOLOGICAL AND IMMUNOHISTOCHEMICAL MANIFESTATIONS OF DIFFERENT VARIANTS OF ACUTE KIDNEY ALLOGRAFT REJECTIONТраилинА.В.TrailinA.Vandrei_trailin@ukr.netНиконенкоТ.Н.NikonenkoT.N.1@ru.ruОстапенкоТ.И.OstapenkoT.I.1@ru.ruПоляковН.Н.PolyakovN.N.1@ru.ruНиконенкоА.С.NikonenkoA.S.1@ru.ruЗапорожская медицинская академия последипломного образования, УкраинаРоссияZaporozhskaya medical academy of postgraduate education, UkrainaRussian FederationЗапорожский межрегиональный центр трансплантации, УкраинаРоссияZaporozhsky interregional transplant center, UkrainaRussian FederationЗапорожский межрегиональный центр трансплантации, Украина Запорожская медицинская академия последипломного образования, УкраинаРоссияZaporozhsky interregional transplant center, Ukraina Zaporozhskaya medical academy of postgraduate education, UkrainaRussian Federation2009230920091122936Copyright © Траилин А., Никоненко Т., Остапенко Т., Поляков Н., Никоненко А., 20092009Траилин А., Никоненко Т., Остапенко Т., Поляков Н., Никоненко А.Trailin A., Nikonenko T., Ostapenko T., Polyakov N., Nikonenko A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/179

Целью исследования была морфологическая характеристика острого отторжения (ОРО) почечных аллотрансплантатов: Т-клеточно- (ОТОО) и антитело-опосредованного (ОАОО). Гистологические срезы биоптатов исследовались после их окрашивания рутинными методами, а также антителами к C4d-компоненту комплемента, CD45R0-антигену Т-лимфоцитов и CD68-антигену макрофагов. Показано, что для ОТОО характерно усиление Т-лимфоцитарной инфильтрации интерстиция по сравнению с дотрансплантационным этапом при сохранении степени макрофагальной инфильтрации, минимальный мононуклеарный гломерулит и капиллярит; тубулит. Морфологическими признаками ОАОО являются: нейтрофильный и Т-лимфоцитарный гломерулит, диффузный капиллярит с дилатацией ПТК, маргинацией моноцитов с примесью нейтрофилов, усиление по сравнению с дотрансплантационным этапом макрофагальной инфильтрации интерстиция при полуколичественной оценке; более высокая по сравнению с ОТОО степень Т-лимфоцитарной и макрофагальной инфильтрации интерстиция с преобладанием лимфоцитов при количественной оценке.

The aim of this study was morphological characteristics of peculiar properties of acute T-cells- (ATMR) and antibody-mediated kidney allograft rejection (AAMR). Histological slices of kidney allograft biopsies were examined after coloration by routine methods and immunohistochemical proceeding for C4d, CD45R0 T-lymphocytes antigen and CD68 macrophages antigen. We’ve determined that the key features of ATMR are: increasing of T-lymphocytes interstitial infi ltration with the same level of macrophages infi ltration comparing with implantation biopsies, mild mononuclear glomerulitis and capillaritis; tubulitis. Morphological traits of AAMR are neutrophilic and T-lymphocytes glomerulitis, diffuse capillaritis, capillaries dilatation and margination of mononuclear cells with admixture of neutrophils, macrophagal interstitial infi ltration under semiquantitative evaluation, as well as, more pronounced, than in ATMR, T-lymphoctes and macrophages interstitial infi ltration under quantitative evaluation.

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The authors declare that there are no conflicts of interest present.