vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2025-1-172-182vtio-1782Research ArticleИмплантаты и искусственные органыImplants and Artificial OrgansОсобенности ремоделирования заплат из полиуретана и ксеноперикарда на примере крупной животной моделиFeatures of polyurethane and xenopericardial patch remodeling using a large animal model as an exampleСенокосоваЕ. А.SenokosovaE. A.

Сенокосова Евгения Андреевна

650002, Кемерово, б-р акад. Л.С. Барбараша, 6. Тел. (951) 611-60-76

Evgeniya Senokosova

6, Bul’var Barbarasha, Kemerovo, 650002  Phone: (951) 611-60-76

sergeewa.ew@yandex.ruПрокудинаЕ. С.ProkudinaE. S.

Кемерово

Kemerovo 

prokes@kemcardio.ruМухамадияровР. А.MukhamadiyarovR. A.

Кемерово

Kemerovo 

muhara@kemcardio.ruВеликановаЕ. А.VelikanovaE. A.

Кемерово

Kemerovo 

veliea@kemcardio.ruКривкинаЕ. О.KrivkinaE. O.

Кемерово

Kemerovo 

kriveo@kemcardio.ruМироновА. В.MironovA. V.

Кемерово

Kemerovo 

miroav@kemcardio.ruСардинЕ. С.SardinE. S.

Кемерово

Kemerovo 

sardes@kemcardio.ruАнтоноваЛ. В.AntonovaL. V.

Кемерово

Kemerovo 

antolv@kemcardio.ruФГБНУ «Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний»РоссияResearch Institute for Complex Issues of Cardiovascular DiseasesRussian Federation202526032025271172182Copyright © Сенокосова Е.А., Прокудина Е.С., Мухамадияров Р.А., Великанова Е.А., Кривкина Е.О., Миронов А.В., Сардин Е.С., Антонова Л.В., 20252025Сенокосова Е.А., Прокудина Е.С., Мухамадияров Р.А., Великанова Е.А., Кривкина Е.О., Миронов А.В., Сардин Е.С., Антонова Л.В.Senokosova E.A., Prokudina E.S., Mukhamadiyarov R.A., Velikanova E.A., Krivkina E.O., Mironov A.V., Sardin E.S., Antonova L.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1782

Цель: провести сравнительную оценку особенностей ремоделирования заплат из полиуретана (ПУ) и бычьего перикарда (БП), имплантированных на 6 месяцев в сонную артерию овцы. Материалы и методы. Синтетические матриксы изготавливали из 12% раствора ПУ в хлороформе методом электроспиннинга на установке Nanon-01A (MECC, Япония). Для сравнения использовали биологические матриксы из БП коммерческого производства (Кем-Периплас Нео, ЗАО «Неокор», Россия). Матриксы имплантировали в виде сосудистых заплат в сонные артерии овцам (n = 3). Срок имплантации составил 6 месяцев. Проходимость артерий с имплантированными сосудистыми протезами оценивали методом ультразвукового исследования. После эксплантации образцы матриксов изучали с помощью гистологического исследования, сканирующей электронной микроскопии и конфокальной микроскопии с предварительной окраской специфическими флюоресцентно меченными антителами. Статистическую обработку данных проводили в программе GraphPad Prism 8. Результаты. Через 6 месяцев имплантации матриксов из ПУ и БП в стенку сонной артерии овец выявлена полная проходимость сосудов без аневризматических расширений, значимых стенозов и гематом. ПУ-матрикс отличился менее выраженной соединительно-тканной капсулой и отсутствием гиперплазии неоинтимы, толщина ремоделированной ПУ-стенки составила 731,2 (711,5; 751,3) мкм. Одновременно выявлена гиперплазия неоинтимы БП, толщина которой составила 627 (538; 817) мкм, а толщина ремоделированной стенки – 1723 (1693; 1772) мкм. Эндотелизация и структурная сохранность ПУ-матрикса были выше в сравнении с БП. Заключение. ПУ-матрикс – новый эффективный материал для сосудистой реконструкции, доказавший способность к гармоничному ремоделированию, биоинертности и структурную сохранность в условиях кровотока в исследовании in vivo на овцах. За счет высоких эластических свойств и долговечности ПУ интересен как монокомпонент, так и в составе композитного материала, пригодного для создания изделий для нужд сердечно-сосудистой хирургии.

Objective: to compare the remodeling features of polyurethane (PU) and bovine pericardium (BP) patches that have been implanted in a sheep carotid artery for 6 months. Materials and methods. Synthetic matrices were fabricated from a 12% PU solution in chloroform by electrospinning on a Nanon-01A machine (MECC, Japan). Biological matrices made from commercially produced PU (Kem-Periplas Neo, CJSC Neocor, Russia) were used for comparison. The matrices were implanted as vascular patches into sheep carotid arteries (n = 3). Implantation period was 6 months. Via ultrasound scan, the patency of arteries bearing the implanted vascular prostheses was evaluated. After removal, the matrix samples were studied by histological examination, scanning electron microscopy and confocal microscopy. Prior to this, they had been stained with specific fluorescently labeled antibodies. The GraphPad Prism 8 application was used to process statistical data. Results. The sheep carotid artery wall was completely patent, with no aneurysmal dilatations, significant stenoses, and hematomas six months after the PU and BP matrices were implanted. The PU matrix was distinguished by a less pronounced connective-tissue capsule and no neointima hyperplasia; the thickness of the remodeled PU wall was 731.2 (711.5; 751.3) μm. At the same time, there was BP neointimal hyperplasia with a thickness of 627 (538; 817) μm and a remodeled wall thickness of 1723 (1693; 1772) μm. In comparison to BP, the PU matrix exhibited greater endothelialization and structural integrity. Conclusion. An in vivo study on sheep demonstrated the potential of PU matrix, a novel and effective material for vascular reconstruction, to maintain harmonious remodeling, bioinertness and structural integrity when in contact with blood. Due to its excellent elastic qualities and durability, PU is interesting both as a monocomponent and as a component of a composite material that can be used to create products for the needs of cardiovascular surgery.

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The authors declare that there are no conflicts of interest present.