vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2024-2-135-144vtio-1724Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesПроблема получения клеточной культуры эндотелиальных клеток роговицы для регенеративных целейChallenges of obtaining cultured corneal endothelial cells for regenerative purposesОстровскийД. С.OstrovskiD. S.

Островский Дмитрий Сергеевич

127486, г. Москва, Бескудниковский бульвар, д. 59а

Тел. (499) 488-85-58

Dmitriy Ostrovskiy

59a, Beskudnikovsky Bul’var, Moscow, 127486

Phone: (499) 488-85-58

dmitriy.ostrovskiy@gmail.comБорзенокС. А.BorzenokS. A.

Москва

Moscow

mdborzenok@yandex.ruМалюгинБ. Э.MalyuginB. E.

Москва

Moscow

boris.malyugin@gmail.comАнтоноваО. П.AntonovaO. P.

Москва

Moscow

antonova.mntk@gmail.comХубецоваМ. Х.KhubetsovaM. Kh.

Москва

Moscow

porporina@inbox.ruКеримовТ. З.KerimovT. Z.

Москва

Moscow

timkerimov2014@yandex.ruФГАУ «Национальный медицинский исследовательский центр «Межотраслевой научно-технический комплекс «Микрохирургия глаза» имени академика С.Н. Федорова» Минздрава РоссииРоссияFyodorov Eye Microsurgery ComplexRussian FederationФГАУ «Национальный медицинский исследовательский центр «Межотраслевой научно-технический комплекс «Микрохирургия глаза» имени академика С.Н. Федорова» Минздрава России; ФГБОУ ВО «Московский государственный медико-стоматологический университет имени А.И. Евдокимова» Минздрава РоссииРоссияFyodorov Eye Microsurgery Complex; Moscow State University of Medicine and DentistryRussian Federation202431012024262135144Copyright © Островский Д.С., Борзенок С.А., Малюгин Б.Э., Антонова О.П., Хубецова М.Х., Керимов Т.З., 20242024Островский Д.С., Борзенок С.А., Малюгин Б.Э., Антонова О.П., Хубецова М.Х., Керимов Т.З.Ostrovski D.S., Borzenok S.A., Malyugin B.E., Antonova O.P., Khubetsova M.K., Kerimov T.Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1724

Клетки заднего эпителия роговицы человека, или эндотелия, обладают ограниченной пролиферативной активностью как in vivo так и in vitro. Повреждение или дисфункция этих клеток приводит к нарушению прозрачности роговицы различной степени выраженности, вплоть до слепоты. В настоящее время единственным действенным методом лечения патологии эндотелия является трансплантация донорской роговицы, содержащей пул здоровых и функционально активных клеток. Однако существует глобальная нехватка донорских роговиц, что приводит к неудовлетворенной клинической потребности и тому, что хирургическое лечение получает лишь 1 пациент из 10 нуждающихся. В связи с этим создание клеточных конструкций и искусственных аналогов роговицы человека, содержащих здоровый эндотелий, является весьма актуальной задачей современной офтальмо-трансплантологии. В данном обзоре представлены текущее состояние вопроса, сложности и перспективы получении клеточной культуры эндотелиальных клеток роговицы in vitro для целей трансплантации.

Human posterior corneal epithelium (corneal endothelium) has limited proliferative activity both in vivo and in vitro. Disease or dysfunction in these cells leads to impaired corneal transparency of varying degrees of severity, up to blindness. Currently, the only effective standard treatment for corneal endothelial dysfunction is transplantation of donor cornea that contains a pool of healthy and functionally active cells. However, there is a global shortage of donor corneas, which has led to an unmet clinical need and the fact that only 1 patient out of 10 in need receives surgical treatment. Therefore, creation of cellular constructs and artificial human corneas containing healthy endothelium is a very urgent challenge facing modern ophthalmic transplantology. This review presents the current state of affairs, challenges and prospects for obtaining cultured corneal endothelial cells (CECs) in vitro for transplantation purposes.

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The authors declare that there are no conflicts of interest present.