vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2023-3-106-112vtio-1666Research ArticleДонорство органовOrgan DonationРазработка нового малообъемного оксигенатора и создание гидродинамического стенда для ex vivo перфузии легких на мелких животныхDevelopment of a new low-volume oxygenator and creation of a hydrodynamic test bench for ex vivo lung perfusion in small animalsЕсиповаО. Ю.EsipovaO. Yu.

Есипова Ольга Юрьевна,

123182, Москва, ул. Щукинская, д. 1

Olga Esipova,

1, Shchukinskaya str., Moscow, 123182

olgadmitrieva2008@yandex.ruБогдановВ. К.BogdanovV. K.

Москва

Moscow

bogdanovv@bk.ruЕсиповА. С.EsipovA. S.

Красногорск

Krasnogorsk

alexeyesipov@mail.ruКулешовА. П.KuleshovA. P.

Москва

Moscow

ilovemylene@yandex.ruБучневА. С.BuchnevA. S.

Москва

Moscow

labbts@mail.ruВолковаЕ. А.VolkovaE. A.

Москва

Moscow

ДробышевА. А.DrobyshevA. A.

Москва

Moscow

labbts@mail.ruГрудининН. В.GrudininN. V.

Москва

Moscow

zbignev.religa@mail.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian FederationФГБУ «Национальный медицинский исследовательский центр высоких медицинских технологий имени А.А. Вишневского» Минобороны РоссииРоссияVishnevsky Central Military Clinical HospitalRussian Federation202328062023253106112Copyright © Есипова О.Ю., Богданов В.К., Есипов А.С., Кулешов А.П., Бучнев А.С., Волкова Е.А., Дробышев А.А., Грудинин Н.В., 20232023Есипова О.Ю., Богданов В.К., Есипов А.С., Кулешов А.П., Бучнев А.С., Волкова Е.А., Дробышев А.А., Грудинин Н.В.Esipova O.Y., Bogdanov V.K., Esipov A.S., Kuleshov A.P., Buchnev A.S., Volkova E.A., Drobyshev A.A., Grudinin N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1666

В фундаментальных исследованиях широко используются модели мелких животных. Однако экспериментальные гидродинамические стенды, в состав которых входят экстракорпоральные контуры, часто имеют ограничения, связанные с размерами и объемами заполнения оборудования. Таким образом, мы стремились разработать и апробировать миниатюрный оксигенатор, а также малообъемную гидродинамическую систему для проведения ex vivo перфузии легких мелких животных. Была разработана и изготовлена серия малообъемных мембранных оксигенаторов (n = 10) с 90–100 выровненными микропористыми полипропиленовыми полыми волокнами, помещенными внутрь оболочки, запечатанной с обоих концов для изоляции перфузирующего раствора. Благодаря такой конструкции газ проходит через полые волокна, в то время как перфузат циркулирует вокруг волокон. Был разработан и собран гидродинамический стенд с малым объемом заполнения для проведения изолированной ex vivo перфузии легких, а также оценки рабочих характеристик оксигенаторов: потока газа и перфузата, перфузионного давления и температуры при диапазоне расхода 5–70 мл/мин.

Small animal models are widely used in basic research. However, experimental hydrodynamic test benches, which include extracorporeal circuits, often have limitations associated with the size and filling volume of equipment. Thus, we aimed at developing and validating a miniature oxygenator as well as a low-volume hydrodynamic system for ex vivo perfusion of small animal lungs. A series of low-volume membrane oxygenators (n = 10) with 90–100 aligned microporous polypropylene hollow fibers, placed inside a sheath that is sealed at both ends to isolate the perfusing solution, was designed and manufactured. This design makes gas to flow through the hollow fibers and perfusate to circulate around the fibers. A low-volume hydrodynamic test bench was designed and assembled for isolated ex vivo lung perfusion and for evaluation of the performance characteristics of the oxygenators: gas and perfusate flow, perfusion pressure and temperature at 5–70 ml/min flow range.

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The authors declare that there are no conflicts of interest present.