vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2023-2-82-98vtio-1626Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesДецеллюляризованная строма пуповины в тканевой инженерии и регенеративной медицине: систематический обзорDecellularized umbilical cord stroma in tissue engineering and regenerative medicine: a systematic reviewБасокЮ. Б.BasokYu. B.

Басок Юлия Борисовна

123182, Москва, ул. Щукинская, д. 1. Тел. (499) 190-45-90

Yulia Basok

1, Shchukinskaya str., Moscow, 123182, Phone: (499) 190-45-90

bjb2005@mail.ruКондратенкоА. А.KondratenkoA. A.

Санкт-Петербург

St. Petersburg

kondraa24@gmail.comКалюжнаяЛ. И.KalyuzhnayaL. I.

Москва

St. Petersburg

terrestra@mail.ruВолковаЕ. А.VolkovaE. A.

Москва

Moscow

vorobyov_doc@mail.ruВоробьевК. А.VorobyovK. A.

Санкт-Петербург

St. Petersburg

no-reply@subs.elpub.ruСевастьяновВ. И.SevastianovV. I.

Москва

Moscow

ФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian FederationФГБВОУ ВО «Военно-медицинская академия имени С.М. Кирова»РоссияKirov Military Medical AcademyRussian FederationФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияKirov Military Medical AcademyRussian Federation2023150720232528298Copyright © Басок Ю.Б., Кондратенко А.А., Калюжная Л.И., Волкова Е.А., Воробьев К.А., Севастьянов В.И., 20232023Басок Ю.Б., Кондратенко А.А., Калюжная Л.И., Волкова Е.А., Воробьев К.А., Севастьянов В.И.Basok Y.B., Kondratenko A.A., Kalyuzhnaya L.I., Volkova E.A., Vorobyov K.A., Sevastianov V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1626

Несмотря на большой прогресс в области создания биоматериалов для тканевой инженерии и регенеративной медицины, высокие требования, предъявляемые к искусственным матриксам (матрицам, носителям, скаффолдам), являются причиной продолжающегося поиска природных или синтетических миметиков внеклеточного матрикса. Среди таких материалов многообещающим представляется децеллюляризованная строма пуповины благодаря высокому содержанию гиалуроновой кислоты, цитокинов и факторов роста, а также отсутствию этических ограничений для ее получения. Описано, что децеллюляризованная строма пуповины способствует восстановлению хряща, печеночной ткани, нервной ткани и заживлению ран. В обзоре дан критический анализ и обобщены опубликованные данные, касающиеся способности децеллюляризованной стромы пуповины поддерживать необходимые условия для адгезии, миграции, дифференцировки и функциональной активности адгезированных клеток, стимулируя, таким образом, внутренний (физиологический) регенеративный потенциал тканей. Поиск литературы проводился в электронных базах данных Medline/PubMed (www/ncbi.nlm.nih.gov/pubmed), Cochrane library (https://www.cochrane.org), eLIBRARY / Российский индекс научного цитирования (https://www.elibrary.ru). Критериями включения было присутствие биоматериалов, полученных из децеллюляризованной стромы пуповины. Критериями исключения статей были такие объекты исследования, как децеллюляризованные сосуды (вены и артерии) пуповины, а также культуры клеток пуповины. Для анализа получаемых продуктов, областей их применения, методов децеллюляризации и результатов исследований были выбраны 25 оригинальных статей на английском и русском языках. Также в обзоре обсуждаются перспективы использования децеллюляризованной пуповины в медицине

Despite great progress in the field of biomaterials for tissue engineering and regenerative medicine, the high requirements placed on artificial matrices (matrices, carriers, scaffolds) are the reason for the ongoing search for natural or synthetic extracellular matrix mimetics. Among such materials, decellularized umbilical cord (UC) stroma appears to be very attractive – it has a high content of hyaluronic acid, cytokines, and growth factors, and there are no ethical restrictions for its production. Decellularized UC stroma has been found to promote cartilage, liver tissue and nerve tissue repair, as well as wound healing. The review critically analyzes and summarizes published data on the ability of decellularized UC stroma to maintain the necessary conditions for adhesion, migration, differentiation and functional activity of adherent cells, thus stimulating the internal (physiological) regenerative potential of tissues. Literature was searched for in the following electronic databases: Medline/PubMed (www/ncbi. nlm.nih.gov/pubmed), Cochrane library (https://www.cochrane.org), and eLIBRARY/Russian Science Citation Index (https://www.elibrary.ru). Inclusion criteria were the presence of biomaterials obtained from decellularized human UC stroma. Exclusion criteria for papers included research objects as decellularized umbilical cord vessels (veins and arteries) and umbilical cord cell cultures. Twenty-five original articles in English and Russian were selected for analysis of the products obtained, their applications, decellularization methods and research results. The review also discusses the prospects for decellularized umbilical cord in medicine.

строма пуповиныдецеллюляризациявнеклеточный матриксрегенеративная медицинатканевая инженерияumbilical cord stromadecellularizationextracellular matrixregenerative medicinetissue engineeringReferencesСевастьянов ВИ. Технологии тканевой инженерии и регенеративной медицины. Вестник трансплантологии и искусственных органов. 2014; 16 (3): 93–108. doi: 10.15825/1995-1191-2014-3-93-108.Sevast’yanov VI. Tekhnologii tkanevoy inzhenerii i regenerativnoy meditsiny. Vestnik transplantologii i iskusstvennykh organov. 2014; 16 (3): 93–108. doi: 10.15825/1995-1191-2014-3-93-108.Мелешина АВ, Быстрова АС, Роговая ОС, Воротеляк ЕА, Васильев АВ, Загайнова ЕВ. Тканеинженерные конструкты кожи и использование стволовых клеток для создания кожных эквивалентов. Современные технологии в медицине. 2017; 9 (1): 198–220. doi: 10.17691/stm2017.9.1.24.Meleshina AV, Bystrova AS, Rogovaya OS, Vorotelyak YeA, Vasil’yev AV, Zagaynova YeV. Tkaneinzhenernyye konstrukty kozhi i ispol’zovaniye stvolovykh kletok dlya sozdaniya kozhnykh ekvivalentov. Sovremennyye tekhnologii v meditsine. 2017; 9 (1): 198–220. doi: 10.17691/stm2017.9.1.24.Фоминых ЕМ, Митрофанов НВ, Живцов ОП, Стручков АА, Зубрицкий ВФ, Лебедева ЮН и др. Трансплантация тканевых эквивалентов в лечении некоторых повреждений кожи. Вестник трансплантологии и искусственных органов. 2020; 22 (1): 165–173. doi: 10.15825/19952020-1-165-173.Fominykh YeM, Mitrofanov NV, Zhivtsov OP, Struchkov AA, Zubritskiy VF, Lebedeva YuN i dr. Transplantatsiya tkanevykh ekvivalentov v lechenii nekotorykh povrezhdeniy kozhi. Vestnik transplantologii i iskusstvennykh organov. 2020; 22 (1): 165–173. doi: 10.15825/19952020-1-165-173.Севастьянов ВИ, Григорьев АМ, Басок ЮБ, Кирсанова ЛА, Василец НВ, Малкова АП и др. Биосовместимые и матриксные свойства полилактидных губок. Вестник трансплантологии и искусственных органов. 2018; 20 (2): 82–90. doi: 10.15825/1995-1191-2018-2-82-90.Sevast’yanov VI, Grigor’ yev AM, Basok YuB, Kirsanova LA, Vasilets NV, Malkova AP i dr. Biosovmestimyye i matriksnyye svoystva polilaktidnykh gubok. Vestnik transplantologii i iskusstvennykh organov. 2018; 20 (2): 82–90. doi: 10.15825/1995-1191-2018-2-82-90.Liu H, Gong Y, Zhang K, Ke S, Wang Y, Wang J, Wang H. Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting. Gels. 2023; 9 (3): 195. doi: 10.3390/gels9030195.Liu H, Gong Y, Zhang K, Ke S, Wang Y, Wang J, Wang H. Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting. Gels. 2023; 9 (3): 195. doi: 10.3390/gels9030195.Басок ЮБ, Севастьянов ВИ. Технологии тканевой инженерии и регенеративной медицины в лечении дефектов хрящевой ткани суставов. Вестник трансплантологии и искусственных органов. 2016; 18 (4): 102–122. doi: 10.15825/1995-1191-2016-4-102-122.Basok YuB, Sevast’yanov VI. Tekhnologii tkanevoy inzhenerii i regenerativnoy meditsiny v lechenii defektov khryashchevoy tkani sustavov. Vestnik transplantologii i iskusstvennykh organov. 2016; 18 (4): 102– 122. doi: 10.15825/1995-1191-2016-4-102-122.Sevastianov VI, Basok YB, Grigor’ev AM, Kirsanova LA, Vasilets VN. Formation of tissue-engineered construct of human cartilage tissue in a flow-through bioreactor. Bulletin of Experimental Biology and Medicine. 2017; 164: 269–273. doi: 10.1007/s10517-017-3971-z.Sevastianov VI, Basok YB, Grigor’ev AM, Kirsanova LA, Vasilets VN. Formation of tissue-engineered construct of human cartilage tissue in a flow-through bioreactor. Bulletin of Experimental Biology and Medicine. 2017; 164: 269–273. doi: 10.1007/s10517-017-3971-z.Севастьянов ВИ, Басок ЮБ, Григорьев АМ, Кирсанова ЛА, Василец НВ. Перфузионный биореактор для создания тканеинженерных конструкций. Медицинская техника. 2017; 303 (3): 9–11.Sevast’yanov VI, Basok YuB, Grigor’yev AM, Kirsanova LA, Vasilets NV. Perfuzionnyy bioreaktor dlya sozdaniya tkaneinzhenernykh konstruktsiy. Meditsinskaya tekhnika. 2017; 303 (3): 9–11.Badylak SF. Decellularized allogeneic and xenogeneic tissue as a bioscaffold for regenerative medicine: factors that influence the host response. Annals of Biomedical Engineering. 2014; 42 (7): 1517–1527. doi: 10.1007/s10439-013-0963-7. PMID: 24402648.Badylak SF. Decellularized allogeneic and xenogeneic tissue as a bioscaffold for regenerative medicine: factors that influence the host response. Annals of Biomedical Engineering. 2014; 42 (7): 1517–1527. doi: 10.1007/s10439-013-0963-7. PMID: 24402648.Bakhtyar N, Jeschke MG, Mainville L, Herer E, AminiNik S. Acellular gelatinous material of human umbilical cord enhances wound healing: a candidate remedy for deficient wound healing. Frontiers in Physiology. 2017; 8: 200. doi: 10.3389/fphys.2017.00200. PMID: 28421003.Bakhtyar N, Jeschke MG, Mainville L, Herer E, AminiNik S. Acellular gelatinous material of human umbilical cord enhances wound healing: a candidate remedy for deficient wound healing. Frontiers in Physiology. 2017; 8: 200. doi: 10.3389/fphys.2017.00200. PMID: 28421003.Gupta P, Chaudhuri GR, Janani G, Agarwala M, Ghosh D, Nandi SK, Mandal BB. Functionalized silk vascular grafts with decellularized human Wharton’s jelly improves remodeling via immunomodulation in rabbit jugular vein. Advanced Healthcare Materials. 2021; 10 (19): e2100750. doi: 10.1002/adhm.202100750. PMID: 34378360.Gupta P, Chaudhuri GR, Janani G, Agarwala M, Ghosh D, Nandi SK, Mandal BB. Functionalized silk vascular grafts with decellularized human Wharton’s jelly improves remodeling via immunomodulation in rabbit jugular vein. Advanced Healthcare Materials. 2021; 10 (19): e2100750. doi: 10.1002/adhm.202100750. PMID: 34378360.Калюжная ЛИ, Чернов ВЕ, Фрумкина АС, Чеботарев СВ, Земляной ДА, Товпеко ДВ, Косулин АВ. Изготовление тканеинженерного бесклеточного матрикса пуповины человека. Вестник Российской военно­медицинской академии. 2020; 69 (1): 124–130. doi: 10.17816/brmma25980.Kalyuzhnaya LI, Chernov VE, Frumkina AS, Chebotarev SV, Zemlyanoy DA, Tovpeko DV, Kosulin AV. Izgotovleniye tkaneinzhenernogo beskletochnogo matriksa pupoviny cheloveka. Vestnik Rossiyskoy voyenno­meditsinskoy akademii. 2020; 69 (1): 124–130. doi: 10.17816/brmma25980.Sobolewski K, Małkowski A, Bańkowski E, Jaworski S. Wharton’s jelly as a reservoir of peptide growth factors. Placenta. 2005; 26 (10): 747–752. doi: 10.1016/j.placenta.2004.10.008. PMID: 16226124.Sobolewski K, Małkowski A, Bańkowski E, Jaworski S. Wharton’s jelly as a reservoir of peptide growth factors. Placenta. 2005; 26 (10): 747–752. doi: 10.1016/j.placenta.2004.10.008. PMID: 16226124.Gupta A, El­Amin SF, Levy HJ, Sze­Tu R, Ibim SE, Maffulli N. Umbilical cord-derived Wharton’s jelly for regenerative medicine applications. Journal of Orthopaedic Surgery. 2020; 15. doi: 10.1186/s13018-020-15537. PMID: 32054483.Gupta A, El­Amin SF, Levy HJ, Sze­Tu R, Ibim SE, Maffulli N. Umbilical cord-derived Wharton’s jelly for regenerative medicine applications. Journal of Orthopaedic Surgery. 2020; 15. doi: 10.1186/s13018-020-15537. PMID: 32054483.Fayon A, Helle D, Francius G. Characterization of an innovative biomaterial derived from human Wharton’s jelly as a new promising coating for tissue engineering applications. Frontiers in Bioengineering and Biotechnology. 2022; 10: 884069. doi: 10.3389/fbioe.2022.884069. PMID: 35769101.Fayon A, Helle D, Francius G. Characterization of an innovative biomaterial derived from human Wharton’s jelly as a new promising coating for tissue engineering applications. Frontiers in Bioengineering and Biotechnology. 2022; 10: 884069. doi: 10.3389/fbioe.2022.884069. PMID: 35769101.Dubus M, Scomazzon L, Chevrier J, Ledouble C, Baldit A, Braux J et al. Antibacterial and immunomodulatory properties of acellular Wharton’s jelly matrix. Biomedicines. 2022; 10 (2): 227. doi: 10.3390/biomedicines10020227. PMID: 35203437.Dubus M, Scomazzon L, Chevrier J, Ledouble C, Baldit A, Braux J et al. Antibacterial and immunomodulatory properties of acellular Wharton’s jelly matrix. Biomedicines. 2022; 10 (2): 227. doi: 10.3390/biomedicines10020227. PMID: 35203437.Dubus M, Scomazzon L, Chevrier J, Montanede A, Baldit A, Terryn C et al. Decellularization of Wharton’s jelly increases its bioactivity and antibacterial properties. Frontiers in Bioengineering and Biotechnology. 2022; 10: 828424. doi: 10.3389/fbioe.2022.828424. PMID: 35360386.Dubus M, Scomazzon L, Chevrier J, Montanede A, Baldit A, Terryn C et al. Decellularization of Wharton’s jelly increases its bioactivity and antibacterial properties. Frontiers in Bioengineering and Biotechnology. 2022; 10: 828424. doi: 10.3389/fbioe.2022.828424. PMID: 35360386.Ramzan F, Ekram S, Frazier T, Salim A, Mohiuddin OA, Khan I. Decellularized human umbilical tissue derived hydrogels promote proliferation and chondrogenic differentiation of mesenchymal stem cells. Bioengineering. 2022; 9: 239. doi: 10.3390/bioengineering9060239.Ramzan F, Ekram S, Frazier T, Salim A, Mohiuddin OA, Khan I. Decellularized human umbilical tissue derived hydrogels promote proliferation and chondrogenic differentiation of mesenchymal stem cells. Bioengineering. 2022; 9: 239. doi: 10.3390/bioengineering9060239.Kehtari M, Beiki B, Zeynali B. Decellularized Wharton’s jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells. Journal of Cellular Biochemistry. 2019; 120 (4): 6683–6697. doi: 10.1002/jcb.27965. PMID: 30417406.Kehtari M, Beiki B, Zeynali B. Decellularized Wharton’s jelly extracellular matrix as a promising scaffold for promoting hepatic differentiation of human induced pluripotent stem cells. Journal of Cellular Biochemistry. 2019; 120 (4): 6683–6697. doi: 10.1002/jcb.27965. PMID: 30417406.Kočí Z, Výborný K, Dubišová J. Extracellular matrix hydrogel derived from human umbilical cord as a scaffold for neural tissue repair and its comparison with extracellular matrix from porcine tissues. Tissue Engineering Part C Methods. 2017; 23 (6): 333–345. doi: 10.1089/ten.TEC.2017.0089. PMID: 28471271.Kočí Z, Výborný K, Dubišová J. Extracellular matrix hydrogel derived from human umbilical cord as a scaffold for neural tissue repair and its comparison with extracellular matrix from porcine tissues. Tissue Engineering Part C Methods. 2017; 23 (6): 333–345. doi: 10.1089/ten.TEC.2017.0089. PMID: 28471271.Lu J­H, Hsia K, Su CK, Pan YH, Ma H, Chiou SH, Lin CH. A novel dressingcomposed of adipose stem cells and decellularized Wharton’s jelly facilitatedwound healing and relieved lymphedema by enhancing angiogenesis and lymphangiogenesis in a rat model. Journal of functional biomaterials. 2023; 14 (2): 104. doi: 10.3390/jfb14020104. PMID: 36826903.Lu J­H, Hsia K, Su CK, Pan YH, Ma H, Chiou SH, Lin CH. A novel dressingcomposed of adipose stem cells and decellularized Wharton’s jelly facilitatedwound healing and relieved lymphedema by enhancing angiogenesis and lymphangiogenesis in a rat model. Journal of functional biomaterials. 2023; 14 (2): 104. doi: 10.3390/jfb14020104. PMID: 36826903.Beiki B, Zeynali B, Seyedjafari E. Fabrication of a three dimensional spongy scaffold using human Wharton’s jelly derived extra cellular matrix for wound healing. Materials Science and Engineering C­Materials for Biological Applications. 2017; 78: 627–638. doi: 10.1016/j.msec.2017.04.074. PMID: 28576031.Beiki B, Zeynali B, Seyedjafari E. Fabrication of a three dimensional spongy scaffold using human Wharton’s jelly derived extra cellular matrix for wound healing. Materials Science and Engineering C­Materials for Biological Applications. 2017; 78: 627–638. doi: 10.1016/j.msec.2017.04.074. PMID: 28576031.Bullard JD, Lei J, Lim JJ, Massee M, Fallon AM, Koob TJ. Evaluation of dehydrated human umbilical cord biological properties for wound care and soft tissue healing. Journal of Biomedical Materials Research Part B Applied Biomaterials. 2019; 107 (4): 1035–1046. doi: 10.1002/jbm.b.34196. PMID: 30199609.Bullard JD, Lei J, Lim JJ, Massee M, Fallon AM, Koob TJ. Evaluation of dehydrated human umbilical cord biological properties for wound care and soft tissue healing. Journal of Biomedical Materials Research Part B Applied Biomaterials. 2019; 107 (4): 1035–1046. doi: 10.1002/jbm.b.34196. PMID: 30199609.Li D, Chiu G, Lipe B, Hopkins RA, Lillis J, Ashton JM et al. Decellularized Wharton jelly matrix: a biomimetic scaffold for ex vivo hematopoietic stem cell culture. Blood Advances. 2019; 3 (7): 1011–1026. doi: 10.1182/ bloodadvances.2018019315. PMID: 30940636.Li D, Chiu G, Lipe B, Hopkins RA, Lillis J, Ashton JM et al. Decellularized Wharton jelly matrix: a biomimetic scaffold for ex vivo hematopoietic stem cell culture. Blood Advances. 2019; 3 (7): 1011–1026. doi: 10.1182/ bloodadvances.2018019315. PMID: 30940636.Penolazzi L, Pozzobon M, Bergamin LS, D’Agostino S, Francescato R, Bonaccorsi G et al. Extracellular matrix from decellularized Wharton’s jelly improves the behavior of cells from degenerated intervertebral disc. Frontiers in Bioengineering and Biotechnology. 2020; 8: 262. doi: 10.3389/fbioe.2020.00262. PMID: 32292779.Penolazzi L, Pozzobon M, Bergamin LS, D’Agostino S, Francescato R, Bonaccorsi G et al. Extracellular matrix from decellularized Wharton’s jelly improves the behavior of cells from degenerated intervertebral disc. Frontiers in Bioengineering and Biotechnology. 2020; 8: 262. doi: 10.3389/fbioe.2020.00262. PMID: 32292779.Jadalannagari S, Converse G, McFall C, Buse E, Filla M, Villar MT et al. Decellularized Wharton’s jelly from human umbilical cord as a novel 3d scaffolding material for tissue engineering applications. PLoS One. 2017; 12 (2): e0172098. doi: 10.1371/journal.pone.0172098. PMID: 28222169.Jadalannagari S, Converse G, McFall C, Buse E, Filla M, Villar MT et al. Decellularized Wharton’s jelly from human umbilical cord as a novel 3d scaffolding material for tissue engineering applications. PLoS One. 2017; 12 (2): e0172098. doi: 10.1371/journal.pone.0172098. PMID: 28222169.PLOS ONE Staff. Correction: Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications. PLoS One. 2017 Mar 7; 12 (3): e0173827. doi: 10.1371/journal.pone.0173827. Erratum for: PLoS One. 2017 Feb 21; 12 (2): e0172098. PMID: 28267774.PLOS ONE Staff. Correction: Decellularized Wharton’s Jelly from human umbilical cord as a novel 3D scaffolding material for tissue engineering applications. PLoS One. 2017 Mar 7; 12 (3): e0173827. doi: 10.1371/journal.pone.0173827. Erratum for: PLoS One. 2017 Feb 21; 12 (2): e0172098. PMID: 28267774.Jalili Z, Emamgolizadeh B, Abbaszadeh H, Jalili S, Derakhshani M, Yousefi M et al. Effect of replacement of Wharton acellular jelly with fbs on the expression of megakaryocyte linear markers in hematopoietic stem cells CD34. Asian Pacific Journal of Cancer Prevention. 2022; 23 (10): 3281–3286. doi: 10.31557/APJCP.2022.23.10.3281. PMID: 36308350.Jalili Z, Emamgolizadeh B, Abbaszadeh H, Jalili S, Derakhshani M, Yousefi M et al. Effect of replacement of Wharton acellular jelly with fbs on the expression of megakaryocyte linear markers in hematopoietic stem cells CD34. Asian Pacific Journal of Cancer Prevention. 2022; 23 (10): 3281–3286. doi: 10.31557/APJCP.2022.23.10.3281. PMID: 36308350.Talebi M, Nozad Charoudeh H, Movassaghpour Akbari AA, Baradaran B, Kazemi T. Acellular Wharton’s jelly, potentials in t-cell subtypes differentiation, activation and proliferation. Advanced Pharmaceutical Bulletin. 2020; 10 (4): 617–622. doi: 10.34172/apb.2020.074. PMID: 33072540.Talebi M, Nozad Charoudeh H, Movassaghpour Akbari AA, Baradaran B, Kazemi T. Acellular Wharton’s jelly, potentials in t-cell subtypes differentiation, activation and proliferation. Advanced Pharmaceutical Bulletin. 2020; 10 (4): 617–622. doi: 10.34172/apb.2020.074. PMID: 33072540.Dan P, Velot É, Francius G, Menu P, Decot V. Humanderived extracellular matrix from Wharton’s jelly: An untapped substrate to build up a standardized and homogeneous coating for vascular engineering. Acta Biomaterialia. 2017; 48: 227–237. doi: 10.1016/j.actbio.2016.10.018. PMID: 27769940.Dan P, Velot É, Francius G, Menu P, Decot V. Humanderived extracellular matrix from Wharton’s jelly: An untapped substrate to build up a standardized and homogeneous coating for vascular engineering. Acta Biomaterialia. 2017; 48: 227–237. doi: 10.1016/j.actbio.2016.10.018. PMID: 27769940.Basiri A, Farokhi M, Azami M, Ebrahimi­Barough S, Mohamadnia A, Rashtbar M et al. A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering. Progress in Biomaterials. 2019; 8 (1): 31–42. doi: 10.1007/s40204-019-0108-7. PMID: 30706299.Basiri A, Farokhi M, Azami M, Ebrahimi­Barough S, Mohamadnia A, Rashtbar M et al. A silk fibroin/decellularized extract of Wharton’s jelly hydrogel intended for cartilage tissue engineering. Progress in Biomaterials. 2019; 8 (1): 31–42. doi: 10.1007/s40204-019-0108-7. PMID: 30706299.Azarbarz N, Khorsandi L, Nejaddehbashi F, Neisi N, Nejad DB. Decellularized Wharton’s jelly scaffold enhances differentiation of mesenchymal stem cells to insulin-secreting cells. Tissue Cell. 2022; 79: 101938. doi: 10.1016/j.tice.2022.101938. PMID: 36152380.Azarbarz N, Khorsandi L, Nejaddehbashi F, Neisi N, Nejad DB. Decellularized Wharton’s jelly scaffold enhances differentiation of mesenchymal stem cells to insulin-secreting cells. Tissue Cell. 2022; 79: 101938. doi: 10.1016/j.tice.2022.101938. PMID: 36152380.Xiao T, Guo W, Chen M, Hao C, Gao S, Huang J et al. Fabrication and In Vitro study of tissue-engineered cartilage scaffold derived from Wharton’s jelly extracellular matrix. BioMed Research International. 2017; 2017: 5839071. doi: 10.1155/2017/5839071. PMID: 29214173.Xiao T, Guo W, Chen M, Hao C, Gao S, Huang J et al. Fabrication and In Vitro study of tissue-engineered cartilage scaffold derived from Wharton’s jelly extracellular matrix. BioMed Research International. 2017; 2017: 5839071. doi: 10.1155/2017/5839071. PMID: 29214173.Foltz KM, Neto AE, Francisco JC, Simeoni RB, Miggiolaro AFRDS, do Nascimento TG et al. Decellularized Wharton jelly implants do not trigger collagen and cartilaginous tissue production in tracheal injury in rabbits. Life (Basel). 2022; 12 (7): 942. doi: 10.3390/ life12070942. PMID: 35888031.Foltz KM, Neto AE, Francisco JC, Simeoni RB, Miggiolaro AFRDS, do Nascimento TG et al. Decellularized Wharton jelly implants do not trigger collagen and cartilaginous tissue production in tracheal injury in rabbits. Life (Basel). 2022; 12 (7): 942. doi: 10.3390/ life12070942. PMID: 35888031.Yuan Z, Cao F, Gao C, Yang Z, Guo Q, Wang Y. Decellularized human umbilical cord Wharton jelly scaffold improves tendon regeneration in a rabbit rotator cuff tendon defect model. The American Journal of Sports Medicine. 2022; 50 (2): 371–383. doi: 10.1177/03635465211055722. PMID: 34739346.Yuan Z, Cao F, Gao C, Yang Z, Guo Q, Wang Y. Decellularized human umbilical cord Wharton jelly scaffold improves tendon regeneration in a rabbit rotator cuff tendon defect model. The American Journal of Sports Medicine. 2022; 50 (2): 371–383. doi: 10.1177/03635465211055722. PMID: 34739346.Mann LK, Won JH, Trenton NJ, Garnett J, Snowise S, Fletcher SA et al. Cryopreserved human umbilical cord versus acellular dermal matrix patches for in utero fetal spina bifida repair in a pregnant rat model. Journal of neurosurgery. Spine. 2019; 32 (2): 321–331. doi: 10.3171/2019.7.SPINE19468. PMID: 31675701.Mann LK, Won JH, Trenton NJ, Garnett J, Snowise S, Fletcher SA et al. Cryopreserved human umbilical cord versus acellular dermal matrix patches for in utero fetal spina bifida repair in a pregnant rat model. Journal of neurosurgery. Spine. 2019; 32 (2): 321–331. doi: 10.3171/2019.7.SPINE19468. PMID: 31675701.Converse GL, Li D, Buse EE, Hopkins RA, Aljitawi OS. Wharton’s jelly matrix decellularization for tissue engineering applications. Methods in Molecular Biology. 2018; 1577: 25–33. doi: 10.1007/7651_2017_61. PMID: 28786033.Converse GL, Li D, Buse EE, Hopkins RA, Aljitawi OS. Wharton’s jelly matrix decellularization for tissue engineering applications. Methods in Molecular Biology. 2018; 1577: 25–33. doi: 10.1007/7651_2017_61. PMID: 28786033.Výborný K, Vallová J, Kočí Z, Kekulová K, Jiráková K, Jendelová P et al. Genipin and EDC crosslinking of extracellular matrix hydrogel derived from human umbilical cord for neural tissue repair. Scientific Reports. 2019; 9 (1): 10674. doi: 10.1038/s41598-019-47059-x. PMID: 31337821.Výborný K, Vallová J, Kočí Z, Kekulová K, Jiráková K, Jendelová P et al. Genipin and EDC crosslinking of extracellular matrix hydrogel derived from human umbilical cord for neural tissue repair. Scientific Reports. 2019; 9 (1): 10674. doi: 10.1038/s41598-019-47059-x. PMID: 31337821.Болгарчук ОО, Калюжная ЛИ, Чернов ВЕ, Чеботарев СВ, Симанов АГ, Чернуцкий АМ. Оптимизация процедуры децеллюляризации соединительной ткани пуповины человека для создания тканеинженерного раневого покрытия. Известия Российской военно­медицинской академии. 2020; 39 (S3-1): 13–18.Bolgarchuk OO, Kalyuzhnaya LI, Chernov VE, Chebotarev SV, Simanov AG, Chernutskiy AM. Optimizatsiya protsedury detsellyulyarizatsii soyedinitel’noy tkani pupoviny cheloveka dlya sozdaniya tkaneinzhenernogo ranevogo pokrytiya. Izvestiya Rossiyskoy voyenno­meditsinskoy akademii. 2020; 39 (S3-1): 13–18.Кондратенко АА, Калюжная ЛИ, Соколова МО, Чернов ВЕ. Сохранность важнейших структурных компонентов пуповины человека после децеллюляризации как этапа изготовления высокорегенеративного раневого покрытия. Биотехнология. 2021; 37 (5): 61–65. doi: 10.21519/0234-2758-2021-37-5-61-65.Kondratenko AA, Kalyuzhnaya LI, Sokolova MO, Chernov VE. Sokhrannost’ vazhneyshikh strukturnykh komponentov pupoviny cheloveka posle detsellyulyarizatsii kak etapa izgotovleniya vysokoregenerativnogo ranevogo pokrytiya. Biotekhnologiya. 2021; 37 (5): 61– 65. doi: 10.21519/0234-2758-2021-37-5-61-65.Чеботарев СВ, Калюжная ЛИ, Хоминец ВВ, Чернов ВЕ, Фрумкина АС, Земляной ДА и др. Регенеративные эффекты гидрогеля из биоматериала пуповины человека в восстановлении повреждений суставного хряща. Клиническая и экспериментальная хирургия. Журнал имени академика Б.В. Петровского. 2020; 8 (4): 119–125. doi: 10.33029/2308-1198-2020-84-119-125.Chebotarev SV, Kalyuzhnaya LI, Khominets VV, Chernov VE, Frumkina AS, Zemlyanoy DA i dr. Regenerativnyye effekty gidrogelya iz biomateriala pupoviny cheloveka v vosstanovlenii povrezhdeniy sustavnogo khryashcha. Klinicheskaya i eksperimental’naya khirurgiya. Zhurnal imeni akademika B.V. Petrovskogo. 2020; 8 (4): 119–125. doi: 10.33029/2308-1198-2020-84-119-125.Кондратенко АА, Товпеко ДВ, Калюжная ЛИ. Биологические эффекты бесклеточного тканеинженерного продукта из пуповины человека. Патогенез. 2022; 20 (4): 53–62. doi: 10.25557/23100435.2022.04.53-62.Kondratenko AA, Tovpeko DV, Kalyuzhnaya LI. Biologicheskiye effekty beskletochnogo tkaneinzhenernogo produkta iz pupoviny cheloveka. Patogenez. 2022; 20 (4): 53–62. doi: 10.25557/23100435.2022.04.53-62.Калюжная ЛИ, Чеботарев СВ. Гидрогель из пуповины человека в лечении дефектов суставногохряща в эксперименте. Известия Российской военно­медицинской академии. 2020; 39 (S3-1): 37–40.Ka lyuzh naya LI, Chebotarev SV. Gidrogel’ iz pupoviny cheloveka v lechenii defektov sustavnogo khryashcha v eksperimente. Izvestiya Rossiyskoy voyenno­meditsinskoy akademii. 2020; 39 (S3-1): 37–40.Калюжная ЛИ, Хоминец ВВ, Чеботарев СВ, Харкевич ОН, Кудяшев АЛ, Чернов ВЕ и др. Применение биоматериала из пуповины человека для восстановления повреждений суставного хряща. Профилактическая и клиническая медицина. 2019; 4 (73): 45–52.Kalyuzhnaya LI, Khominets VV, Chebotarov SV, Kharkevich ON, Kudyashev AL, Chernov VE i dr. Primeneniye biomateriala iz pupoviny cheloveka dlya vosstanovleniya povrezhdeniy sustavnogo khryashcha. Profilakticheskaya i klinicheskaya meditsina. 2019; 4 (73): 45–52.Кондратенко АА, Калюжная­Земляная ЛИ, Товпеко ДВ, Шевелева ВС, Глушаков РИ. Биологические и функциональные свойства лиофилизированных форм тканеинженерных матриксов из пуповины человека. Вестник трансплантологии и искусственных органов. 2023; 25 (1): 113–122. doi: 10.15825/1995-1191-2023-1-113-122.Kondratenko AA, Kalyuzhnaya­Zemlyanaya LI, Tovpeko DV, Sheveleva VS, Glushakov RI. Biologicheskiye i funktsional’nyye svoystva liofilizirovannykh form tkaneinzhenernykh matriksov iz pupoviny cheloveka. Vestnik transplantologii i iskusstvennykh organov. 2023; 25 (1): 113–122. doi: 10.15825/1995-1191-2023-1-113-122.Shin H, Jo S, Mikos AG. Biomimetic materials for tissue engineering. Biomaterials. 2003; 24 (24): 4353– 4364. doi: 10.1016/s0142-9612(03)00339-9. PMID: 12922148.Shin H, Jo S, Mikos AG. Biomimetic materials for tissue engineering. Biomaterials. 2003; 24 (24): 4353– 4364. doi: 10.1016/s0142-9612(03)00339-9. PMID: 12922148.Немец ЕА, Лажко АЭ, Григорьев АМ, Басок ЮБ, Кириллова АД, Севастьянов ВИ. Биосовместимые и функциональные свойства тканеспецифической мелкодисперсной 3D-матрицы из децеллюляризованного хряща свиньи. Вестник трансплантологии и искусственных органов. 2022; 24 (4): 73–84. doi: 10.15825/1995-1191-2022-4-73-84.Nemets YeA, Lazhko AE, Grigor’yev AM, Basok YuB, Kirillova AD, Sevast’yanov VI. Biosovmestimyye i funktsional’nyye svoystva tkanespetsificheskoy melkodispersnoy 3Dmatritsy iz detsellyulyarizovannogo khryashcha svin’i. Vestnik transplantologii i iskusstvennykh organov. 2022; 24 (4): 73–84. doi: 10.15825/1995-1191-2022-4-73-84.Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. 2011; 32 (12): 3233–3243. doi: 10.1016/j.biomaterials.2011.01.057. PMID: 21296410.Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. 2011; 32 (12): 3233–3243. doi: 10.1016/j.biomaterials.2011.01.057. PMID: 21296410.Simsa R, Padma AM, Heher P, Hellström M, Teuschl A, Jenndahl L et al. Systematic in vitro comparison of decellularization protocols for blood vessels. PLoS One. 2018; 13 (12): e0209269. doi: 10.1371/journal.pone.0209269. PMID: 30557395.Simsa R, Padma AM, Heher P, Hellström M, Teuschl A, Jenndahl L et al. Systematic in vitro comparison of decellularization protocols for blood vessels. PLoS One. 2018; 13 (12): e0209269. doi: 10.1371/journal.pone.0209269. PMID: 30557395.Sevastianov VI, Basok YuB, Grigoriev AM, Nemets EA, Kirillova AD, Kirsanova LA et al. Decellularization of cartilage microparticles: Effects of temperature, supercritical carbon dioxide and ultrasound on biochemical, mechanical, and biological properties. Journal of Biomedical Materials Research A. 2023; 111 (4): 543–555. doi: 10.1002/jbm.a.37474. PMID: 36478378.Sevastianov VI, Basok YuB, Grigoriev AM, Nemets EA, Kirillova AD, Kirsanova LA et al. Decellularization of cartilage microparticles: Effects of temperature, supercritical carbon dioxide and ultrasound on biochemical, mechanical, and biological properties. Journal of Biomedical Materials Research A. 2023; 111 (4): 543–555. doi: 10.1002/jbm.a.37474. PMID: 36478378.Kirillova AD, Basok YuB, Lazhko AE, Grigoryev AM, Kirsanova LA, Nemets EA, Sevastianov VI. Creating a tissue-specific microdispersed matrix from a decellularized porcine liver. Inorganic Materials: Applied Research. 2021; 12 (3): 812–819. doi: 10.1134/S2075113321030199.Kirillova AD, Basok YuB, Lazhko AE, Grigoryev AM, Kirsanova LA, Nemets EA, Sevastianov VI. Creating a tissue-specific microdispersed matrix from a decellularized porcine liver. Inorganic Materials: Applied Research. 2021; 12 (3): 812–819. doi: 10.1134/S2075113321030199.Целуйко СС, Малюк ЕА, Корнеева ЛС, Красавина НП. Морфофункциональная характеристика дермы кожи и ее изменения при старении (обзор литературы). Бюллетень физиологии и патологии дыхания. 2016; 60. doi: 10.12737/20130.Целуйко СС, Малюк ЕА, Корнеева ЛС, Красавина НП. Морфофункциональная характеристика дермы кожи и ее изменения при старении (обзор литературы). Бюллетень физиологии и патологии дыхания. 2016; 60. Tseluyko SS, Malyuk YeA, Korneyeva LS, Krasavina NP. Morfofunktsional’naya kharakteristika dermy kozhi i yeye izmeneniya pri starenii (obzor literatury). Byulleten’ fiziologii i patologii dykhaniya. 2016; 60. doi: 10.12737/20130.Басок ЮБ, Кириллова АД, Григорьев АМ, Кирсанова ЛА, Немец ЕА, Севастьянов ВИ. Получение микродисперсного тканеспецифического децеллюляризованного матрикса из суставного хряща свиньи. Перспективные материалы. 2020; 5: 51–60. doi: 10.30791/1028-978x-2020-5-51-60.Basok YuB, Kirillova AD, Grigoriev AM, Kirsanova LA, Nemets EA, Sevastianov VI. Fabrication of microdispersed tissue-specific decellularized matrix from porcine articular cartilage. Perspektivnye materialy. 2020; 5: 51–60. doi: 10.30791/1028-978x-2020-5-51-60.Потекаев НН, Фриго НВ, Петерсен ЕВ. Искусственная кожа: виды, области применения. Клиническая дерматология и венерология. 2017; 6: 7–15. doi: 10.17116/klinderma20171667-15.Potekayev NN, Frigo NV, Petersen YeV. Iskusstvennaya kozha: vidy, oblasti primeneniya. Klinicheskaya dermatologiya i venerologiya. 2017; 6: 7–15. doi: 10.17116/klinderma20171667-15.Cramer MC, Badylak SF. Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior. Annals of Biomedical Engineering. 2020; 48 (7): 2132–2153. doi: 10.1007/s10439-019-02408-9. PMID: 31741227.Cramer MC, Badylak SF. Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior. Annals of Biomedical Engineering. 2020; 48 (7): 2132–2153. doi: 10.1007/s10439-019-02408-9. PMID: 31741227.Galili U. Acceleration of wound healing by α-gal nanoparticles interacting with the natural anti-Gal antibody. Journal of Immunology Research. 2015; 2015: 589648. doi: 10.1155/2015/589648. PMID: 25922849.Galili U. Acceleration of wound healing by α-gal nanoparticles interacting with the natural anti-Gal antibody. Journal of Immunology Research. 2015; 2015: 589648. doi: 10.1155/2015/589648. PMID: 25922849.Dziki JL, Huleihel L, Scarritt ME, Badylak SF. Extracellular matrix bioscaffolds as immunomodulatory biomaterials. Tissue Engineering Part A. 2017; 23 (19–20): 1152–1159. doi: 10.1089/ten.TEA.2016.0538. PMID: 28457179.Dziki JL, Huleihel L, Scarritt ME, Badylak SF. Extracellular matrix bioscaffolds as immunomodulatory biomaterials. Tissue Engineering Part A. 2017; 23 (19–20): 1152–1159. doi: 10.1089/ten.TEA.2016.0538. PMID: 28457179.Sicari BM, Johnson SA, Siu BF, Crapo PM, Daly KA, Jiang H et al. The effect of source animal age upon the in vivo remodeling characteristics of an extracellular matrix scaffold. Biomaterials. 2012; 33 (22): 5524–5533. doi: 10.1016/j.biomaterials.2012.04.017. PMID: 22575834.Sicari BM, Johnson SA, Siu BF, Crapo PM, Daly KA, Jiang H et al. The effect of source animal age upon the in vivo remodeling characteristics of an extracellular matrix scaffold. Biomaterials. 2012; 33 (22): 5524–5533. doi: 10.1016/j.biomaterials.2012.04.017. PMID: 22575834.Huleihel L, Hussey GS, Naranjo JD, Zhang L, Dziki JL, Turner NJ et al. Matrix-bound nanovesicles within ECM bioscaffolds. Science Advances. 2016; 2 (6): e1600502. doi: 10.1126/sciadv.1600502. PMID: 27386584.Huleihel L, Hussey GS, Naranjo JD, Zhang L, Dziki JL, Turner NJ et al. Matrix-bound nanovesicles within ECM bioscaffolds. Science Advances. 2016; 2 (6): e1600502. doi: 10.1126/sciadv.1600502. PMID: 27386584.Delgado LM, Bayon Y, Pandit A, Zeugolis DI. To crosslink or not to cross-link? Cross-linking associated foreign body response of collagen-based devices. Tissue Engineering Part B Reviews. 2015; 21 (3): 298–313. doi: 10.1089/ten.TEB.2014.0290. PMID: 25517923.Delgado LM, Bayon Y, Pandit A, Zeugolis DI. To crosslink or not to cross-link? Cross-linking associated foreign body response of collagen-based devices. Tissue Engineering Part B Reviews. 2015; 21 (3): 298–313. doi: 10.1089/ten.TEB.2014.0290. PMID: 25517923.Roy A, Mantay M, Brannan C, Griffiths S. Placental Tissues as Biomaterials in Regenerative Medicine. BioMed Research International. 2022; 2022: 6751456. doi: 10.1155/2022/6751456. PMID: 35496035.Roy A, Mantay M, Brannan C, Griffiths S. Placental Tissues as Biomaterials in Regenerative Medicine. BioMed Research International. 2022; 2022: 6751456. doi: 10.1155/2022/6751456. PMID: 35496035.Lo DD, Zimmermann AS, Nauta A, Longaker MT, Lorenz HP. Scarless fetal skin wound healing update. Birth Defects Research Part C Embryo Today Reviews. 2012; 96 (3): 237–247. doi: 10.1002/bdrc.21018. PMID: 23109319.Lo DD, Zimmermann AS, Nauta A, Longaker MT, Lorenz HP. Scarless fetal skin wound healing update. Birth Defects Research Part C Embryo Today Reviews. 2012; 96 (3): 237–247. doi: 10.1002/bdrc.21018. PMID: 23109319.Yannas IV. Similarities and differences between induced organ regeneration in adults and early foetal regeneration. Journal of the Royal Society Interface. 2005; 2 (5): 403–417. doi: 10.1098/rsif.2005.0062. PMID: 16849201.Yannas IV. Similarities and differences between induced organ regeneration in adults and early foetal regeneration. Journal of the Royal Society Interface. 2005; 2 (5): 403–417. doi: 10.1098/rsif.2005.0062. PMID: 16849201.Nanaev AK, Kohnen G, Milovanov AP, Domogatsky SP, Kaufmann P. Stromal differentiation and architecture of the human umbilical cord. Placenta. 1997; 18 (1): 53–64. doi: 10.1016/s0143-4004(97)90071-0. PMID: 9032810.Nanaev AK, Kohnen G, Milovanov AP, Domogatsky SP, Kaufmann P. Stromal differentiation and architecture of the human umbilical cord. Placenta. 1997; 18 (1): 53–64. doi: 10.1016/s0143-4004(97)90071-0. PMID: 9032810.Franc S, Rousseau JC, Garrone R, van der Rest M, Moradi­Améli M. Microfibrillar composition of umbilical cord matrix: characterization of fibrillin, collagen VI and intact collagen V. Placenta. 1998; 19 (1): 95–104. doi: 10.1016/s0143-4004(98)90104-7. PMID: 9481791.Franc S, Rousseau JC, Garrone R, van der Rest M, Moradi­Améli M. Microfibrillar composition of umbilical cord matrix: characterization of fibrillin, collagen VI and intact collagen V. Placenta. 1998; 19 (1): 95–104. doi: 10.1016/s0143-4004(98)90104-7. PMID: 9481791.Калюжная ЛИ, Соколова МО, Чернов ВЕ, Земляной ДА, Чеботарев СВ, Чалисова НИ и др. Влияние бесклеточного матрикса пуповины человека на динамику роста и жизнеспособность культивируемых клеток человека и животных ex vivo. Гены и клетки. 2021; 3: 72–79. doi: 10.23868/202110010.Kalyuzhnaya LI, Sokolova MO, Chernov VE, Zemlyanoy DA, Chebotarev SV, Chalisova NI i dr. Vliyaniye beskletochnogo matriksa pupoviny cheloveka na dinamiku rosta i zhiznesposobnost’ kul’tiviruyemykh kletok cheloveka i zhivotnykh ex vivo. Geny i kletki. 2021; 3: 72–79. doi: 10.23868/202110010.https://trialsearch.who.int/Trial2.aspx?TrialID=IRCT 20210612051545N3 [Internet]. World Health Organization: International Clinical Trials Registry Platform (ICTRP). [updated 2022 November 21; cited 2023 April 03]. Available from: https://trialsearch.who.int/Trial2.as px?TrialID=IRCT20210612051545N3.https://trialsearch.who.int/Trial2.aspx?TrialID=IRCT 20210612051545N3 [Internet]. World Health Organization: International Clinical Trials Registry Platform (ICTRP). [updated 2022 November 21; cited 2023 April 03]. Available from: https://trialsearch.who.int/Trial2.as px?TrialID=IRCT20210612051545N3.https://trialsearch.who.int/Trial2.aspx?TrialID=IRCT 20210612051545N2 [Internet]. World Health Organization: International Clinical Trials Registry Platform (ICTRP). [updated 2022 November 21; cited 2023 April 03]. Available from: https://trialsearch.who.int/Trial2.as px?TrialID=IRCT20210612051545N2.https://trialsearch.who.int/Trial2.aspx?TrialID=IRCT 20210612051545N2 [Internet]. World Health Organization: International Clinical Trials Registry Platform (ICTRP). [updated 2022 November 21; cited 2023 April 03]. Available from: https://trialsearch.who.int/Trial2.as px?TrialID=IRCT20210612051545N2.

The authors declare that there are no conflicts of interest present.