vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2013-1-106-117vtio-147Research ArticleОбзор литературыLiterature ReviewsПЕРСПЕКТИВЫ ПРИМЕНЕНИЯ АНТИСМЫСЛОВОЙ ГЕННОЙ ТЕРАПИИ ПРИ ТРАНСПЛАНТАЦИИ ОРГАНОВPERSPECTIVES OF ANTISENSE GENE THERAPY IN ORGAN TRANSPLANTATIONРезникО. Н.ReznikO. N.СкворцовА. Е.SkvortsovA. E.КузьминД. О.KuzminD. O.ТутинА. П.TutinA. P.РезникА. О.ReznikA. O.ГБОУ ВПО «СПбГМУ им. акад. И.П. Павлова», Санкт-ПетербургSt. Petersburg State Medical University named after Academician I.P. Pavlov201327052014151ВЕСТНИК ТРАНСПЛАНТОЛОГИИ И ИСКУССТВЕННЫХ ОРГАНОВ том XV No 1–2013106117Copyright © Резник О.Н., Скворцов А.Е., Кузьмин Д.О., Тутин А.П., Резник А.О., 20132013Резник О.Н., Скворцов А.Е., Кузьмин Д.О., Тутин А.П., Резник А.О.Reznik O.N., Skvortsov A.E., Kuzmin D.O., Tutin A.P., Reznik A.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/147

Главной проблемой трансплантологии остается дефицит донорских органов. Использование для транс- плантации органов, полученных от доноров с расширенными критериями, позволяет расширить донор- ский пул, увеличивая доступность трансплантации нуждающимся в ней пациентам с терминальными стадиями болезней внутренних органов. Основным препятствием к широкому использованию доноров с расширенными критериями является ишемическое повреждение, следующая за ним ишемически- реперфузионная травма органов, активация сценариев программируемой клеточной гибели, приводящие к снижению функционального резерва трансплантатов, а зачастую к развитию тяжелых осложнений в послеоперационном периоде. Применение антисмысловой генной терапии при трансплантации может послужить терапевтическим инструментом, позволяющим влиять на трансплантат на всех этапах работы с ним, улучшая его качество, и следовательно, результат трансплантации. 

Global organ shortage is the crucial point of transplantation nowadays. Usage of expanded criteria donors represents reliable source of donor organs, making transplantation more accessible for patients with end stage organs failure. Ischemia-reperfusion injury followed by the activation of programmed cell death scenarios remains the main obstacle in utilization of marginal grafts. Programmed cell death often leads to life threatening complications in posttransplant period. Antisense gene therapy could provide a therapeutic tool, capable to improve quality of grafts and, consequently, transplantation outcomes. 

антисмысловая генная терапиятрансплантация органовantisense gene therapyorgan transplantationReferencesСПИСОК ЛИТЕРАТУРЫСПИСОК ЛИТЕРАТУРЫСкулачев В.П. Явление запрограммированной смер- ти. Митохондрии, клетки и органы: роль активных форм кислорода // Соросовский образовательный журнал. 2001. T. 7. No 6. С. 1–10.Скулачев В.П. Явление запрограммированной смер- ти. Митохондрии, клетки и органы: роль активных форм кислорода // Соросовский образовательный журнал. 2001. T. 7. No 6. С. 1–10.Иванов К.П. Роль лейкоцитов в динамике микро- циркуляции в норме и при патологии // Вестник Рос- сийской Академии медицинских наук. 2004. No 4. С. 3–13.Иванов К.П. Роль лейкоцитов в динамике микро- циркуляции в норме и при патологии // Вестник Рос- сийской Академии медицинских наук. 2004. No 4. С. 3–13.Allen B., Ilbawi M. Hypoxia, reoxygenation and the role of systemic leukodepletion in pediatric heart surgery // Perfusion. 2001. Vol. 16. P. 19–29.Allen B., Ilbawi M. Hypoxia, reoxygenation and the role of systemic leukodepletion in pediatric heart surgery // Perfusion. 2001. Vol. 16. P. 19–29.Anversa P., Cheng W., Liu Y., Leri A. et al. Apoptosis and myocardial infarction // Basic. Res. Cardiol.1998. Vol. 93 (3). P. 8–12.Anversa P., Cheng W., Liu Y., Leri A. et al. Apoptosis and myocardial infarction // Basic. Res. Cardiol.1998. Vol. 93 (3). P. 8–12.Baranova A.V., Marakhonov A.V., Skoblov M.Yu. siRNA characteristics and properties of it’s nonviral delivery systems // Molecular Biology. 2012. Vol. 46 (3). P. 371– 386.Baranova A.V., Marakhonov A.V., Skoblov M.Yu. siRNA characteristics and properties of it’s nonviral delivery systems // Molecular Biology. 2012. Vol. 46 (3). P. 371– 386.Baranova A.V., Marakhonov A.V., Skoblov M.Yu. Types of non-viral delivery systems of small interfering RNA // Molecular Biology. 2012. Vol. 46 (3). P. 387–401.Baranova A.V., Marakhonov A.V., Skoblov M.Yu. Types of non-viral delivery systems of small interfering RNA // Molecular Biology. 2012. Vol. 46 (3). P. 387–401.Barnhart B.C., Lee J.C., Alappat E.C. et al. The death effector domain protein family // Oncogene. 2003. Vol. 22 (53). P. 8634–8644.Barnhart B.C., Lee J.C., Alappat E.C. et al. The death effector domain protein family // Oncogene. 2003. Vol. 22 (53). P. 8634–8644.Blydt-Hansen T.D. et al. Gene transfer-induced local HO-1 overexpression protects rat kidney transplants from IRI // J. Am. Soc. Nephrol. 2003. Vol. 14. P. 745– 754.Blydt-Hansen T.D. et al. Gene transfer-induced local HO-1 overexpression protects rat kidney transplants from IRI // J. Am. Soc. Nephrol. 2003. Vol. 14. P. 745– 754.Braasch D.A., Paroo Z., Constantinescu A. Biodistribu- tion of phosphodiester and phosphorothioate siRNA // Bioorg. Med. Chem. Lett. 2004. Vol. 14 (5). P. 1139– 1143.Braasch D.A., Paroo Z., Constantinescu A. Biodistribu- tion of phosphodiester and phosphorothioate siRNA // Bioorg. Med. Chem. Lett. 2004. Vol. 14 (5). P. 1139– 1143.Cantarovich F., Heguilén R., Filho M.A. et al. An inter- national opinion poll of well-educated people regarding awareness and feelings about organ donation for trans- plantation // Transpl. Int. 2007. Vol. 20 (6). P. 512–518.Cantarovich F., Heguilén R., Filho M.A. et al. An inter- national opinion poll of well-educated people regarding awareness and feelings about organ donation for trans- plantation // Transpl. Int. 2007. Vol. 20 (6). P. 512–518.Cecka J.M. Donors without a heartbeat // N. Engl. J. Med. 2002. Vol. 347 (4). P. 281–283.Cecka J.M. Donors without a heartbeat // N. Engl. J. Med. 2002. Vol. 347 (4). P. 281–283.Celi A., Pellegrini G., Lorenzet R., De Blasi A., et al. P-selectin induces the expression of tissue factor on monocytes // Proc. Natl. Acad. Sci USA. 1994. Vol. 91 (19). P. 8767–8771.Celi A., Pellegrini G., Lorenzet R., De Blasi A., et al. P-selectin induces the expression of tissue factor on monocytes // Proc. Natl. Acad. Sci USA. 1994. Vol. 91 (19). P. 8767–8771.Celotto A.M., Graveley B.R. Exon-specific RNAi: a tool for dissecting the functional relevance of alternative splicing // RNA. 2002. Vol. 8 (6). P. 718–724.Celotto A.M., Graveley B.R. Exon-specific RNAi: a tool for dissecting the functional relevance of alternative splicing // RNA. 2002. Vol. 8 (6). P. 718–724.Cho W.Y, Terasaki P.I., Cecka J.I. Transplantation of kidneys from donors whose hearts stopped beating // N. Engl. J. Med. 1998. Vol. 338. P. 221–225.Cho W.Y, Terasaki P.I., Cecka J.I. Transplantation of kidneys from donors whose hearts stopped beating // N. Engl. J. Med. 1998. Vol. 338. P. 221–225.Choy J.C., Cruz R.P., Kerjner A. et al. Granzyme B in- duces endothelial cell apoptosis and contributes to the development of transplant vascular disease // Am. J. Transplant. 2005.Vol. 5 (3). P. 494–499.Choy J.C., Cruz R.P., Kerjner A. et al. Granzyme B in- duces endothelial cell apoptosis and contributes to the development of transplant vascular disease // Am. J. Transplant. 2005.Vol. 5 (3). P. 494–499.Clark P.A., Drain M., Malone M.P. Addressing patients’ emotional and spiritual needs // Jt Comm. J. Qual. Saf. 2003. Vol. 29 (12). P. 659–670.Clark P.A., Drain M., Malone M.P. Addressing patients’ emotional and spiritual needs // Jt Comm. J. Qual. Saf. 2003. Vol. 29 (12). P. 659–670.Contreras J.L., Vilatoba M., Eckstein C. et al. Caspase-8 and caspase-3 small interfering RNA decreases ischemia/ reperfusion injury to the liver in mice // Surgery. 2004. Vol. 136 (2). P. 390–400.Contreras J.L., Vilatoba M., Eckstein C. et al. Caspase-8 and caspase-3 small interfering RNA decreases ischemia/ reperfusion injury to the liver in mice // Surgery. 2004. Vol. 136 (2). P. 390–400.Cypel M., de Perrot M., Keshavjee S. et al. Normother- mic ex vivo perfusion prevents lung injury compared to extended cold preservation for transplantation // Am. J. Transplant. 2009. Vol. 9 (10). P. 2262–2269.Cypel M., de Perrot M., Keshavjee S. et al. Normother- mic ex vivo perfusion prevents lung injury compared to extended cold preservation for transplantation // Am. J. Transplant. 2009. Vol. 9 (10). P. 2262–2269.Darwish R.S. Regulatory mechanisms of apoptosis in regularly dividing cells // Cell Health and Cytoskeleton. 2010. Vol. 2. P. 59–68.Darwish R.S. Regulatory mechanisms of apoptosis in regularly dividing cells // Cell Health and Cytoskeleton. 2010. Vol. 2. P. 59–68.David K.K., Sasaki M., Yu S.W. et al. EndoG is dispensable in embryogenesis and apoptosis // Cell. Death. Differ. 2006. Vol. 13 (7). P. 1147–1155.David K.K., Sasaki M., Yu S.W. et al. EndoG is dispensable in embryogenesis and apoptosis // Cell. Death. Differ. 2006. Vol. 13 (7). P. 1147–1155.De Vries A.J., Gu Y.J., Post W.J. et al. Leucocyte depletion during cardiac surgery: a comparison of different filtration strategies // Perfusion. 2003. Vol. 18. P. 31–38.De Vries A.J., Gu Y.J., Post W.J. et al. Leucocyte depletion during cardiac surgery: a comparison of different filtration strategies // Perfusion. 2003. Vol. 18. P. 31–38.de Vries D.K., Lindeman J.H., Tsikas D. et al. Early renal ischemia-reperfusion injury in humans is dominated by IL-6 release from the allograft // Am. J. Transplant. 2009. Vol. 9 (7). P. 1574–1584.de Vries D.K., Lindeman J.H., Tsikas D. et al. Early renal ischemia-reperfusion injury in humans is dominated by IL-6 release from the allograft // Am. J. Transplant. 2009. Vol. 9 (7). P. 1574–1584.Delmonico F.L., Harmon W.E. Use of expanded criteria donors in solid organ transplantation // Curr. Opinion in Organ Transpl. 2000. Vol. 5 (3). P. 227–231.Delmonico F.L., Harmon W.E. Use of expanded criteria donors in solid organ transplantation // Curr. Opinion in Organ Transpl. 2000. Vol. 5 (3). P. 227–231.Delmonico F.L., Dominguez-Gil B., Matesanz R., Noel L. A call for government accountability to achieve national self-sufficiency in organ donation and transplantation // Lancet. 2011. Vol. 378. P. 1414–1418.Delmonico F.L., Dominguez-Gil B., Matesanz R., Noel L. A call for government accountability to achieve national self-sufficiency in organ donation and transplantation // Lancet. 2011. Vol. 378. P. 1414–1418.Diebold S.S., Kaisho T., Hemmi H. Innate antiviral re- sponses by means of TLR7-mediated recognition of single-stranded RNA // Science. 2004. Vol. 303 (5663). P. 1529–1531.Diebold S.S., Kaisho T., Hemmi H. Innate antiviral re- sponses by means of TLR7-mediated recognition of single-stranded RNA // Science. 2004. Vol. 303 (5663). P. 1529–1531.Djamali A., Vidyasagar A., Adulla M. et al. Nox-2 is a modulator of fibrogenensis in kidney allografts // Am. J. Transplant. 2009. Vol. 9. P. 74–82.Djamali A., Vidyasagar A., Adulla M. et al. Nox-2 is a modulator of fibrogenensis in kidney allografts // Am. J. Transplant. 2009. Vol. 9. P. 74–82.Doevendans P.A., Cramer M.J., Lips D.J. et al. Role of apoptosis in reperfusion injury // Cardiovasc. Res. 2004. Vol. 61 (3). P. 414–426.Doevendans P.A., Cramer M.J., Lips D.J. et al. Role of apoptosis in reperfusion injury // Cardiovasc. Res. 2004. Vol. 61 (3). P. 414–426.Dolęgowska B., Blogowski W., Domanski L. Clinical evidence of the association between serum preoperative changes in xanthine metabolizing enzymes activity and early post-transplant kidney allograft function // J. Am. Coll Surg. 2010. Vol. No 211 (5). P. 587–595.Dolęgowska B., Blogowski W., Domanski L. Clinical evidence of the association between serum preoperative changes in xanthine metabolizing enzymes activity and early post-transplant kidney allograft function // J. Am. Coll Surg. 2010. Vol. No 211 (5). P. 587–595.Dumont E.A., Hofstra L., Liem I.H., Boersma H.H. Vi- sualisation of cell death in vivo in patients with acute myocardial infarction // Lancet. 2000. Vol. 356 (9225). P. 209–212.Dumont E.A., Hofstra L., Liem I.H., Boersma H.H. Vi- sualisation of cell death in vivo in patients with acute myocardial infarction // Lancet. 2000. Vol. 356 (9225). P. 209–212.Elbashir S.M., Bechert K., Tuschl T. et al. Identification of essential genes in cultured mammalian cells using small interfering RNAs // J. Cell Sci. 2001. Vol. 114 (24). P. 4557–4565.Elbashir S.M., Bechert K., Tuschl T. et al. Identification of essential genes in cultured mammalian cells using small interfering RNAs // J. Cell Sci. 2001. Vol. 114 (24). P. 4557–4565.Favreau F., Thuillier R., Cau J. et al. Antithrombin the- rapy during warm ischemia and cold preservation pre- vents chronic kidney graft fibrosis in a DCD model // Am. J. Transplant. 2010. Vol. 10. P. 30–39.Favreau F., Thuillier R., Cau J. et al. Antithrombin the- rapy during warm ischemia and cold preservation pre- vents chronic kidney graft fibrosis in a DCD model // Am. J. Transplant. 2010. Vol. 10. P. 30–39.Fattal E., Bochot A. Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA // Adv. Drug. Deliv. Rev. 2006. Vol. 58 (11). P. 1203–1223.Fattal E., Bochot A. Ocular delivery of nucleic acids: antisense oligonucleotides, aptamers and siRNA // Adv. Drug. Deliv. Rev. 2006. Vol. 58 (11). P. 1203–1223.Fernandes E.S., Courade J.P., Keeble J.E. et al. Tumour necrosis factor alpha mediates transient receptor poten- tial vanilloid 1-dependent bilateral thermal hyperalgesia with distinct peripheral roles of interleukin-1beta, protein kinase C and cyclooxygenase-2 signalling // Pain. 2009. Vol. 142 (3). P. 264–274.Fernandes E.S., Courade J.P., Keeble J.E. et al. Tumour necrosis factor alpha mediates transient receptor poten- tial vanilloid 1-dependent bilateral thermal hyperalgesia with distinct peripheral roles of interleukin-1beta, protein kinase C and cyclooxygenase-2 signalling // Pain. 2009. Vol. 142 (3). P. 264–274.Flavell R.A., Alexopoulou L., Holt A.C. et al. Recog- nition of double-stranded RNA and activation of NF- kappaB by Toll-like receptor 3 // Nature. 2001. Vol. 413 (6857). P. 732–738.Flavell R.A., Alexopoulou L., Holt A.C. et al. Recog- nition of double-stranded RNA and activation of NF- kappaB by Toll-like receptor 3 // Nature. 2001. Vol. 413 (6857). P. 732–738.Fondevila C. et al. In vivo normothermic recirculation: an update // Curr. Op. Organ Transplant. 2010. Vol. 15. P. 173–176.Fondevila C. et al. In vivo normothermic recirculation: an update // Curr. Op. Organ Transplant. 2010. Vol. 15. P. 173–176.Friend P.J. et al. Ex vivo normothermic liver perfusion: an update // Curr. Op. Organ. Transplant. 2010. Vol. 15. P. 167–172.Friend P.J. et al. Ex vivo normothermic liver perfusion: an update // Curr. Op. Organ. Transplant. 2010. Vol. 15. P. 167–172.Furuichi K., Yokoyama H., Wada T. Chemokines in renal fibrosis // Contrib. Nephrol. 2003. Vol. 139. P. 66–89.Furuichi K., Yokoyama H., Wada T. Chemokines in renal fibrosis // Contrib. Nephrol. 2003. Vol. 139. P. 66–89.Galluzzi L., Kroemer G., Vandenabeele P. et al. Classi- fication of cell death: recommendations of the Nomen- clature Committee on Cell Death 2009 // Cell. Death. Differ. 2009. Vol. 16 (1). P. 3–11.Galluzzi L., Kroemer G., Vandenabeele P. et al. Classi- fication of cell death: recommendations of the Nomen- clature Committee on Cell Death 2009 // Cell. Death. Differ. 2009. Vol. 16 (1). P. 3–11.Galluzzi L., Maiuri M.C., Vitale I. et al. Cell death modali- ties: classification and pathophysiological implications // Cell. Death. Differ. 2007. Vol. 14 (7). P. 1237–1243.Galluzzi L., Maiuri M.C., Vitale I. et al. Cell death modali- ties: classification and pathophysiological implications // Cell. Death. Differ. 2007. Vol. 14 (7). P. 1237–1243.Galluzzi L., Vitale L., Abrams J.M. et al. Molecular de- finitions of cell death subroutines: recommendations of the nomenclature committee on cell death 2012 // Cell. Death. and Differentiation. 2011. Vol. 19. P. 107–120.Galluzzi L., Vitale L., Abrams J.M. et al. Molecular de- finitions of cell death subroutines: recommendations of the nomenclature committee on cell death 2012 // Cell. Death. and Differentiation. 2011. Vol. 19. P. 107–120.Garcia C.E., Bramhall S., Mirza D.F. Use of marginal donors // Curr. Opinion in Organ Transpl. 2000. Vol. 5. P. 50–56.Garcia C.E., Bramhall S., Mirza D.F. Use of marginal donors // Curr. Opinion in Organ Transpl. 2000. Vol. 5. P. 50–56.Goldstein P., Kroemer G. Cell death by necrosis: towards a molecular definition // Trends. Biochem. Sci. 2007. Vol. 32 (1). P. 37–43.Goldstein P., Kroemer G. Cell death by necrosis: towards a molecular definition // Trends. Biochem. Sci. 2007. Vol. 32 (1). P. 37–43.Gottlieb R.A., Burleson K.O., Kloner R.A. et al. Reperfu- sion injury induces apoptosis in rabbit cardiomyocytes // J. Clin. Invest. 1994. Vol. 94 (4). P. 1621–1628.Gottlieb R.A., Burleson K.O., Kloner R.A. et al. Reperfu- sion injury induces apoptosis in rabbit cardiomyocytes // J. Clin. Invest. 1994. Vol. 94 (4). P. 1621–1628.Granger D.N., Senchenkova E. Leukocyte-Endothelial cell adhesion // Inflammation and the Microcirculation / San Rafael (CA): Morgan & Claypool Life Sciences. 2010. P. 29–38.Granger D.N., Senchenkova E. Leukocyte-Endothelial cell adhesion // Inflammation and the Microcirculation / San Rafael (CA): Morgan & Claypool Life Sciences. 2010. P. 29–38.Graper L. Eine neue Anschauug uber physiologische- Zellausschaltung // Arch. Zeliforsch. 1914. No 12. P. 373–394.Graper L. Eine neue Anschauug uber physiologische- Zellausschaltung // Arch. Zeliforsch. 1914. No 12. P. 373–394.Hebert M., Pallet N., Dieude M. et al. The Molecular Legacy of Apoptosis in Transplantation // Am. J. Trans- plant. 2012. Vol. 12. P. 1378–1384.Hebert M., Pallet N., Dieude M. et al. The Molecular Legacy of Apoptosis in Transplantation // Am. J. Trans- plant. 2012. Vol. 12. P. 1378–1384.Hitomi J., Christofferson D.E. et al. Identification of a molecular signaling network that regulates a cellular necrotic cell death pathway // Cell. 2008. Vol. 135 (7). P. 1311–1323.Hitomi J., Christofferson D.E. et al. Identification of a molecular signaling network that regulates a cellular necrotic cell death pathway // Cell. 2008. Vol. 135 (7). P. 1311–1323.Hoogland E.R., van Heurn L.W., Snoeijs M.G. DCD kidney transplantation results and measures to improve outcome // Curr. Op. Organ Transplant. 2010. Vol. 15. P. 177–182.Hoogland E.R., van Heurn L.W., Snoeijs M.G. DCD kidney transplantation results and measures to improve outcome // Curr. Op. Organ Transplant. 2010. Vol. 15. P. 177–182.Hosgood S.A. et al. Normothermic kidney preservation // Curr. Op. Organ Transplant. 2011. Vol. 16. P. 169–173.Hosgood S.A. et al. Normothermic kidney preservation // Curr. Op. Organ Transplant. 2011. Vol. 16. P. 169–173.Hotchkiss R.S., Strasser A., McDunn J.E. Cell Death // N. Engl. J. Med. 2009. Vol. 361 (16). P. 1570–1583.Hotchkiss R.S., Strasser A., McDunn J.E. Cell Death // N. Engl. J. Med. 2009. Vol. 361 (16). P. 1570–1583.Huang D.C., Adams J.M., Cory S. The conserved N-ter-Huang D.C., Adams J.M., Cory S. The conserved N-ter-minal BH4 domain of Bcl-2 homologues is essential for inhibition of apoptosis and interaction with CED-4 // EMBO J. 1998. Vol. 17 (4). P. 1029–1039.minal BH4 domain of Bcl-2 homologues is essential for inhibition of apoptosis and interaction with CED-4 // EMBO J. 1998. Vol. 17 (4). P. 1029–1039.Jagtap P., Degterev A., Huang Z. et al. Chemical inhibi- tor of nonapoptotic cell death with therapeutic potential for ischemic brain injury // Nat. Chem. Biol. 2005. Vol. 1 (2). P. 112–119.Jagtap P., Degterev A., Huang Z. et al. Chemical inhibi- tor of nonapoptotic cell death with therapeutic potential for ischemic brain injury // Nat. Chem. Biol. 2005. Vol. 1 (2). P. 112–119.Childress J.F., Liverman C.T. Organ Donation: Oppor- tunities for Action // Institute of Medicine (U.S.). Com- mittee on Increasing Rates of Organ Donation. 2006. P. 6–7.Childress J.F., Liverman C.T. Organ Donation: Oppor- tunities for Action // Institute of Medicine (U.S.). Com- mittee on Increasing Rates of Organ Donation. 2006. P. 6–7.Jang H.R., Ko G.J., Wasowska B.A. et al. The interaction between ischemia-reperfusion and immune responses in the kidney // J. Mol. Med. 2009. Vol. 87 (9). P. 859–864.Jang H.R., Ko G.J., Wasowska B.A. et al. The interaction between ischemia-reperfusion and immune responses in the kidney // J. Mol. Med. 2009. Vol. 87 (9). P. 859–864.Kauffman M.H., Bennett L.E., McBride M.A. et al. The expanded donor // Transplant. Rev. 1997. Vol. 11. P. 165–190.Kauffman M.H., Bennett L.E., McBride M.A. et al. The expanded donor // Transplant. Rev. 1997. Vol. 11. P. 165–190.Kim J., Seok Y.M., Jung K.J., Park K.M. Reactive oxy- gen species/oxidative stress contributes to progression of kidney fibrosis following transient ischemic injury in mice // Am. J. Physiol. 2009. Vol. 297. P. F461–F470.Kim J., Seok Y.M., Jung K.J., Park K.M. Reactive oxy- gen species/oxidative stress contributes to progression of kidney fibrosis following transient ischemic injury in mice // Am. J. Physiol. 2009. Vol. 297. P. F461–F470.Koo D.H., Fuggle S.V. Impact of ischemia/reperfusion injury and early inflammatory responses in kidney trans- plantation // Transplantation Rewiews. 2000. Vol. 14 (2). P. 210–224.Koo D.H., Fuggle S.V. Impact of ischemia/reperfusion injury and early inflammatory responses in kidney trans- plantation // Transplantation Rewiews. 2000. Vol. 14 (2). P. 210–224.Koo D.H., Fuggle S.V. The role of mitochondria in ische- mia/reperfusion injury // Transplantation. 2002. Vol. 27. Vol. 73 (4). P. 493–499.Koo D.H., Fuggle S.V. The role of mitochondria in ische- mia/reperfusion injury // Transplantation. 2002. Vol. 27. Vol. 73 (4). P. 493–499.Kootstra G. Asystolic or non-heartbeating donor // Transplantation. 1997. Vol. 63 (7). P. 917–921.Kootstra G. Asystolic or non-heartbeating donor // Transplantation. 1997. Vol. 63 (7). P. 917–921.Kroemer G., Galluzzi L. Necroptosis: a specialized pa- thway of programmed necrosis // Cell. 2008. Vol. 135 (7). P. 1161–1163.Kroemer G., Galluzzi L. Necroptosis: a specialized pa- thway of programmed necrosis // Cell. 2008. Vol. 135 (7). P. 1161–1163.Kroemer G., Green D.R., Ferguson T. et al. Immunogenic and tolerogenic cell death // Nat. Rev. Immunol. 2009. Vol. 9 (5). P. 353–363.Kroemer G., Green D.R., Ferguson T. et al. Immunogenic and tolerogenic cell death // Nat. Rev. Immunol. 2009. Vol. 9 (5). P. 353–363.Kupiec-Weglinski J.W., Zhai Y., Busuttil R.W. Liver ischemia and reperfusion injury: new insights into me- chanisms of innate-adaptive immune-mediated tissue inflammation // Am. J. Transplant. 2011. Vol. 11 (8). P. 11563–11569.Kupiec-Weglinski J.W., Zhai Y., Busuttil R.W. Liver ischemia and reperfusion injury: new insights into me- chanisms of innate-adaptive immune-mediated tissue inflammation // Am. J. Transplant. 2011. Vol. 11 (8). P. 11563–11569.Kwon Y.J. Efficient and targeted delivery of siRNA in vivo // FEBS J. 2010. Vol. 277. P. 4814–4827.Kwon Y.J. Efficient and targeted delivery of siRNA in vivo // FEBS J. 2010. Vol. 277. P. 4814–4827.Land W.G. The role of postischemic reperfusion injury and other nonantigen-dependent inflammatory pathways in transplantation // Transplantation. 2005. Vol. 79. P. 505–514.Land W.G. The role of postischemic reperfusion injury and other nonantigen-dependent inflammatory pathways in transplantation // Transplantation. 2005. Vol. 79. P. 505–514.Ley K., Laudanna C., Cybulsky M.I. et al. Getting to the site of inflammation: the leukocyte adhesion cascade up- dated // Nature. 2007. Vol. 7. P. 678–689.Ley K., Laudanna C., Cybulsky M.I. et al. Getting to the site of inflammation: the leukocyte adhesion cascade up- dated // Nature. 2007. Vol. 7. P. 678–689.Linfert D., Chowdhry T., Rabb H. Lymphocytes and ischemia-reperfusion injury // Transplant Rev. 2009. Vol. 23 (1). P. 1–10.Linfert D., Chowdhry T., Rabb H. Lymphocytes and ischemia-reperfusion injury // Transplant Rev. 2009. Vol. 23 (1). P. 1–10.Lipták P., Kemény E., Morvay Z. et al. Peritubular capil- lary damage in acute humoral rejection: an ultrastructu- ral study on human renal allografts // Am. J. Transplant. 2005. Vol. 5 (12). P. 2870–2876.Lipták P., Kemény E., Morvay Z. et al. Peritubular capil- lary damage in acute humoral rejection: an ultrastructu- ral study on human renal allografts // Am. J. Transplant. 2005. Vol. 5 (12). P. 2870–2876.Lisman T. Activation of coagulation by living donor kidney transplant early after reperfusion // Am. J. Trans- plant. 2010. Vol. 10. P. 434.Lisman T. Activation of coagulation by living donor kidney transplant early after reperfusion // Am. J. Trans- plant. 2010. Vol. 10. P. 434.Luque Gálvez P., Álvarez-Vijande R. Study of cell energy charge in experimental kidney transplant with different warm ischemia periods // Actas. Urol. Esp. 2008. Vol. 32 (1). P. 41–58.Luque Gálvez P., Álvarez-Vijande R. Study of cell energy charge in experimental kidney transplant with different warm ischemia periods // Actas. Urol. Esp. 2008. Vol. 32 (1). P. 41–58.Maltzman J.S., Haase V.H. Low oxygen stimulates the im- mune system // Kidney Int. 2008. Vol. 73 (7). P. 797–799.Maltzman J.S., Haase V.H. Low oxygen stimulates the im- mune system // Kidney Int. 2008. Vol. 73 (7). P. 797–799.Mello C.C., Grishok A., Tabara H. Genetic requirements for inheritance of RNAi in C. elegans // Science. 2000.Mello C.C., Grishok A., Tabara H. Genetic requirements for inheritance of RNAi in C. elegans // Science. 2000.Vol. 287 (5462). P. 2494–2497.Vol. 287 (5462). P. 2494–2497.Metzger R.A., Delmonico F.L., Feng S. et al. ExpandedMetzger R.A., Delmonico F.L., Feng S. et al. Expandedcriteria donors for kidney transplantation // Am. J. Trans-criteria donors for kidney transplantation // Am. J. Trans-plant. 2003. Vol. 3 (4). P. 114–125.plant. 2003. Vol. 3 (4). P. 114–125.Min W., Jevnikar A.M., Chen G. et al. Targeted GeneMin W., Jevnikar A.M., Chen G. et al. Targeted GenePloeg R.J., Bouma H.R., Schuurs T.A. Signal transduc- tion pathways involved in brain death-induced renal in- jury // Am. J. Transplant. 2009. Vol. 9 (5). P. 989–997.Ploeg R.J., Bouma H.R., Schuurs T.A. Signal transduc- tion pathways involved in brain death-induced renal in- jury // Am. J. Transplant. 2009. Vol. 9 (5). P. 989–997.Leuvenink H.G., Ploeg R.J., Damman J. et al. Crosstalk between complement and Toll-like receptor activation in relation to donor brain death and renal ischemia-re- perfusion injury // Am. J. Transplant. 2011. Vol. 11 (4). P. 660–669.Leuvenink H.G., Ploeg R.J., Damman J. et al. Crosstalk between complement and Toll-like receptor activation in relation to donor brain death and renal ischemia-re- perfusion injury // Am. J. Transplant. 2011. Vol. 11 (4). P. 660–669.Leuvenink H.G., Ploeg R.J., Welmoet H. et al. Brain death induced injury // Curr. Op. 2011. Vol. 16 (2).Leuvenink H.G., Ploeg R.J., Welmoet H. et al. Brain death induced injury // Curr. Op. 2011. Vol. 16 (2).Reischl D., Zimmer A. Drug delivery of siRNA therapeu- tics: potentials and limits of nanosystems // Nanomed. Nanotechnol. Biol. Med. 2009. Vol. 5 (1). P. 8–20.Reischl D., Zimmer A. Drug delivery of siRNA therapeu- tics: potentials and limits of nanosystems // Nanomed. Nanotechnol. Biol. Med. 2009. Vol. 5 (1). P. 8–20.Rosengard B.R., Delmonico F.L., Kauffman H.M. et al. Increased transplanted organs from the use of a standar- dized donor management protocol // Am. J. Transplant. 2002. Vol. 2 (8). P. 761–768.Rosengard B.R., Delmonico F.L., Kauffman H.M. et al. Increased transplanted organs from the use of a standar- dized donor management protocol // Am. J. Transplant. 2002. Vol. 2 (8). P. 761–768.Reznik O., Skvortsov A., Moysyuk Y. et al. Kidney from uncontrolled donors after cardiac death with one H warm ischemic time: resuscitation by extracorporal normo- thermic abdominal perfusion «in situ» by leukocytes- free oxygenated blood // Clin. Transplant. 2010. DOI: 10.1111/j.1399-0012.2010.01333.xReznik O., Skvortsov A., Moysyuk Y. et al. Kidney from uncontrolled donors after cardiac death with one H warm ischemic time: resuscitation by extracorporal normo- thermic abdominal perfusion «in situ» by leukocytes- free oxygenated blood // Clin. Transplant. 2010. DOI: 10.1111/j.1399-0012.2010.01333.xRosental R. Organ Donors: Deceased or alive? Quo Vadis? // Annals of Transplantation. 2006. Vol. 11 (3). P. 49–51.Rosental R. Organ Donors: Deceased or alive? Quo Vadis? // Annals of Transplantation. 2006. Vol. 11 (3). P. 49–51.Rowinski W., Kosieradzki M. Ischemia/reperfusion inju- ry in kidney transplantation: mechanisms and preventi- on // Transplant. Proc. 2008. Vol. 40 (10). P. 3279–3288.Rowinski W., Kosieradzki M. Ischemia/reperfusion inju- ry in kidney transplantation: mechanisms and preventi- on // Transplant. Proc. 2008. Vol. 40 (10). P. 3279–3288.Samankatiwat P., Samartzis I., Lertsithichai P. et al. Leu- cocyte depletion in cardiopulmonary bypass: a compari- son of four strategies // Perfusion. 2003. Vol. 18. P. 95–Samankatiwat P., Samartzis I., Lertsithichai P. et al. Leu- cocyte depletion in cardiopulmonary bypass: a compari- son of four strategies // Perfusion. 2003. Vol. 18. P. 95–Silencing of TLR4 Using Liposomal Nanoparticles for 105.Silencing of TLR4 Using Liposomal Nanoparticles for 105.Preventing Liver Ischemia Reperfusion Injury // Am. J.Preventing Liver Ischemia Reperfusion Injury // Am. J.of Transplant. 2011. Vol. 11. P. 1835–1844.of Transplant. 2011. Vol. 11. P. 1835–1844.Min W.P. et al. Novel Small Interfering RNA-Containing Solution Protecting Donor Organs in Heart Transplanta-Min W.P. et al. Novel Small Interfering RNA-Containing Solution Protecting Donor Organs in Heart Transplanta-Sanchez-Fructuoso A.I., Prats D. et al. Non-heart bea- ting donors: experience from the Hospital Clinico of Ma- drid // J. Nephrol. 2003. Vol. 16 (3). P. 387–392.Sanchez-Fructuoso A.I., Prats D. et al. Non-heart bea- ting donors: experience from the Hospital Clinico of Ma- drid // J. Nephrol. 2003. Vol. 16 (3). P. 387–392.Segura T., Tokatlian T. siRNA applications in nanomedi- cine // Nanomed. Nanobiotechnol. 2010. Vol. 2. P. 305–Segura T., Tokatlian T. siRNA applications in nanomedi- cine // Nanomed. Nanobiotechnol. 2010. Vol. 2. P. 305–tion // Circulation. 2009. Vol. 120. P. 1099–1107.tion // Circulation. 2009. Vol. 120. P. 1099–1107.Min W.P. et al. Protection of Renal Ischemia Injury using 315.Min W.P. et al. Protection of Renal Ischemia Injury using 315.Combination Gene Silencing of Complement 3 and Cas- pase 3 Genes // Transplantation. 2006. Vol. 82. P. 1781– 1786.Combination Gene Silencing of Complement 3 and Cas- pase 3 Genes // Transplantation. 2006. Vol. 82. P. 1781– 1786.Moers C., Leuvenink H.G.D., Ploeg R.J. Non-heart bea- ting organ donation: overview and future perspectives // Transplant. International. 2007. Vol. 20 (7). P. 567–575.Moers C., Leuvenink H.G.D., Ploeg R.J. Non-heart bea- ting organ donation: overview and future perspectives // Transplant. International. 2007. Vol. 20 (7). P. 567–575.Mone T.D. The business of organ procurement // Curr. Opin. Organ Transplant. 2002. Vol. 7. P. 60–64.Mone T.D. The business of organ procurement // Curr. Opin. Organ Transplant. 2002. Vol. 7. P. 60–64.Nicholson M.L. et al. Naked small interfering RNA of caspase-3 in preservation solution and autologous blood perfusate protects isolated ischemic porcine kidneys // Transplantation. 2011. Vol. 91 (5). P. 501–507.Nicholson M.L. et al. Naked small interfering RNA of caspase-3 in preservation solution and autologous blood perfusate protects isolated ischemic porcine kidneys // Transplantation. 2011. Vol. 91 (5). P. 501–507.Nicholson M.L., Yates P.J., Hosgood S.A. Leukocyte and platelet depletion improves blood flow and function in a renal transplant model // J. Surg. Res. 2012. Vol. No 172 (1). P. 159–164.Nicholson M.L., Yates P.J., Hosgood S.A. Leukocyte and platelet depletion improves blood flow and function in a renal transplant model // J. Surg. Res. 2012. Vol. No 172 (1). P. 159–164.Novitzky D. Donor management: state of the art // Trans- plant Proc. 1997. Vol. 29 (8). P. 3773–3775.Novitzky D. Donor management: state of the art // Trans- plant Proc. 1997. Vol. 29 (8). P. 3773–3775.Ollinger R., Pratschke J. Role of heme oxygenase-1 in transplantation // Transpl. Int. 2010. Vol. 23 (11). P. 1071–1081.Ollinger R., Pratschke J. Role of heme oxygenase-1 in transplantation // Transpl. Int. 2010. Vol. 23 (11). P. 1071–1081.Sharpe C.C., Wang J.H., Hendry B.M. Silencing genes in the kidney: antisense or RNA interference? // Nephrol. Dial. Transplant. 2008. Vol. 23 (7). P. 2115–2118.Sharpe C.C., Wang J.H., Hendry B.M. Silencing genes in the kidney: antisense or RNA interference? // Nephrol. Dial. Transplant. 2008. Vol. 23 (7). P. 2115–2118.Simmons M.N., Schreiber M.J., Gill I.S. Surgical renal ischemia: a contemporary overview // J. Urol. 2008. Vol. 180 (1). P. 19–30.Simmons M.N., Schreiber M.J., Gill I.S. Surgical renal ischemia: a contemporary overview // J. Urol. 2008. Vol. 180 (1). P. 19–30.Snoeijs M.G., Winkens B., Heemskerk M.B. et al. Kid- ney transplantation from donors after cardiac death: a 25-year experience // Transplantation. 2010. Vol. 90. P. 1106–1112Snoeijs M.G., Winkens B., Heemskerk M.B. et al. Kid- ney transplantation from donors after cardiac death: a 25-year experience // Transplantation. 2010. Vol. 90. P. 1106–1112Sprague A.H., Khalil R.A. Inflammatory cytokines in va- scular dysfunction and vascular disease // Biochem Phar- macol. 2009. Vol. 78 (6). P. 539–552.Sprague A.H., Khalil R.A. Inflammatory cytokines in va- scular dysfunction and vascular disease // Biochem Phar- macol. 2009. Vol. 78 (6). P. 539–552.Steen S., Ingemansson R., Lindstedt S. et al. Compara- tive outcome of double lung transplantation using con- ventional donor lungs and non-acceptable donor lungs reconditioned ex vivo // Interact. Cardiovasc. Thorac. Surg. 2011. Vol. 12 (2). P. 162–165.Steen S., Ingemansson R., Lindstedt S. et al. Compara- tive outcome of double lung transplantation using con- ventional donor lungs and non-acceptable donor lungs reconditioned ex vivo // Interact. Cardiovasc. Thorac. Surg. 2011. Vol. 12 (2). P. 162–165.Stokman G., Qin Y., Racz Z. et al. Application of siRNA in targeting protein expression in kidney desease // Adv. Drug Deliv Rev. 2010. Vol. 62. P. 1378–1389.Stokman G., Qin Y., Racz Z. et al. Application of siRNA in targeting protein expression in kidney desease // Adv. Drug Deliv Rev. 2010. Vol. 62. P. 1378–1389.

The authors declare that there are no conflicts of interest present.