vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2021-1-150-156vtio-1326Research ArticleОБЗОРЫ ЛИТЕРАТУРЫLITERATURE REVIEWSСовременные тенденции в создании бесклеточных алло- и ксенотканей для реконструкции структур сердцаCurrent trends in the creation of cell-free allo- and xenotissues for reconstruction of heart structuresБабенкоС. И.BabenkoS. I.

121552, Москва, Рублевское шоссе, д. 135.

135, Rublevskoe shosse, Moscow, 121552.

МуратовР. М.MuratovR. M.

121552, Москва, Рублевское шоссе, д. 135.

135, Rublevskoe shosse, Moscow, 121552.

СоркомовМ. Н.SorcomovM. N.

Соркомов Максим Нюргустанович.

121552, Москва, Рублевское шоссе, д. 135.

Тел. (495) 414-78-49

Maxim Sorcomov.

135, Rublevskoe shosse, Moscow, 121552.

Phone: (495) 414-78-49

sorcommn@gmail.comНациональный медицинский исследовательский центр сердечно-сосудистой хирургии имени А.Н. Бакулева Минздрава РоссииРоссияBakulev National Medical Research Center for Cardiovascular SurgeryRussian Federation202110042021231150156Copyright © Бабенко С.И., Муратов Р.М., Соркомов М.Н., 20212021Бабенко С.И., Муратов Р.М., Соркомов М.Н.Babenko S.I., Muratov R.M., Sorcomov M.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1326

Тканевая инженерия обладает значительным потенциалом для решения проблем долговечности биологических тканей при использовании в реконструктивной хирургии структур сердца и сосудов. В целях получения биоматериала, морфологически и функционально близкого к поврежденной ткани сердца человека, была предложена технология децеллюляризации. В обзоре рассматриваются различные аспекты и модели децеллюляризации биологических тканей, в том числе современная технология использования сверкритического диоксида углерода как наиболее экологичного и перспективного метода.

Tissue engineering has significant potential for solving the problems of durability of biological tissues when used in cardiac and vascular reconstructive surgery. A decellularization technology has been proposed for obtaining a biomaterial, morphologically and functionally similar to the damaged human heart tissue. This review discusses various aspects and models of biological tissue decellularization, including the modern technology of using supercritical carbon dioxide as the most eco-friendly and promising method.

 

клапан сердцатканевая инженериядецеллюляризациясверхкритический диоксид углеродаheart valvetissue engineeringdecellularizationsupercritical carbon dioxideReferencesАкатов ВС, Муратов РМ, Фадеева ИС, Сачков АС, Бритиков ДВ, Фесенко НИ и др. Изучение биосовместимости трансплантантов клапанов сердца, девитализированных антикальцинозным способом. Гены & Клетки. 2010; V (2): 36-41.Akatov VS, Muratov RM, Fadeeva IS, Sachkov AS, Britikov DV, Fesenko NI i dr. Izuchenie biosovmestimosti transplantantov klapanov serdtsa, devitalizirovannykh antikal'tsinoznym sposobom. Geny & Kletki. 2010; V (2): 36-41.Курапеев ДИ, Лаврешин АВ, Анисимов СВ. Тканевая инженерия клапанов сердца: децеллюризация алло-и ксенографтов. Гены & Клетки. 2012; VII (1): 34-39.Kurapeev DI, Lavreshin AV, Anisimov SV. Tkanevaya inzheneriya klapanov serdtsa: detsellyurizatsiya allo- i ksenograftov. Geny & Kletki. 2012; VII (1): 34-39.Sawada K, Terada D, Yamaoka T, Kitamura S, Fujisa-to T. Cell removal with supercritical carbon dioxide for acellular artificial tissue. J Chem Technol Biotechnol. 2008; 83: 943-949.Sawada K, Terada D, Yamaoka T, Kitamura S, Fujisa-to T. Cell removal with supercritical carbon dioxide for acellular artificial tissue. J Chem Technol Biotechnol. 2008; 83: 943-949.Courtman DW, Pereira CA, Kashef V, McComb D, Lee JM, Wilson GJ. Development of a pericardial acellular matrix biomaterial: Biochemical and mechanical effects of cell extraction. Journal of Biomedical Materials Research. 1994 Jun; 28 (Issue 6): 655-666. https://doi.org/10.1002/jbm.820280602.Courtman DW, Pereira CA, Kashef V, McComb D, Lee JM, Wilson GJ. Development of a pericardial acellular matrix biomaterial: Biochemical and mechanical effects of cell extraction. Journal of Biomedical Materials Research. 1994 Jun; 28 (Issue 6): 655-666. https://doi.org/10.1002/jbm.820280602.Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006; 27: 3675-3683. doi: 10.1016/j.biomaterials.2006.02.014.Gilbert TW, Sellaro TL, Badylak SF. Decellularization of tissues and organs. Biomaterials. 2006; 27: 3675-3683. doi: 10.1016/j.biomaterials.2006.02.014.Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. Volume 32, Issue 12, April 2011, Pages 32333243. doi: 10.1016/j.biomaterials.2011.01.057.Crapo PM, Gilbert TW, Badylak SF. An overview of tissue and whole organ decellularization processes. Biomaterials. Volume 32, Issue 12, April 2011, Pages 32333243. doi: 10.1016/j.biomaterials.2011.01.057.(Gil-Ramirez A, Rosmark O, Spegel P, Sward K, Westergren-Thorsson G, Larsson-Callerfelt A-K, Rodriguez-Meizosocorresponding I. Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes. Sci Rep. 2020; 10: 4031. Published online 2020 Mar 4. https://doi.org/10.1038/s41598-020-60827-4.(Gil-Ramirez A, Rosmark O, Spegel P, Sward K, Westergren-Thorsson G, Larsson-Callerfelt A-K, Rodriguez-Meizosocorresponding I. Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes. Sci Rep. 2020; 10: 4031. Published online 2020 Mar 4. https://doi.org/10.1038/s41598-020-60827-4.Bechtel JF, Muller-Steinhardt M, Schmidtke C, Bruswik A, Stierle U, Sievers HH. Evaluation of the decellularized pulmonary valve homograft (SynerGraft). J Heart Valve Dis. 2003; 12: 734-739.Bechtel JF, Muller-Steinhardt M, Schmidtke C, Bruswik A, Stierle U, Sievers HH. Evaluation of the decellularized pulmonary valve homograft (SynerGraft). J Heart Valve Dis. 2003; 12: 734-739.Cebotari S, Mertsching H, Kallenbach K, Kostin S, Repin O, Batrinac A et al. Construction of Autologous Human Heart Valves Based on an Acellular Allograft Matrix. Circulation. 2002; 106: I-63-I-68.Cebotari S, Mertsching H, Kallenbach K, Kostin S, Repin O, Batrinac A et al. Construction of Autologous Human Heart Valves Based on an Acellular Allograft Matrix. Circulation. 2002; 106: I-63-I-68.Tudorache I, Cebotari S, Sturz G, Kirsch L, Hurschler C, Hilfiker A et al. Tissue Engineering of Heart Valves: Biomechanical and Morphological Properties of Decellularized Heart Valves. J Heart Valve Dis. 2007 Sep; 16 (5): 567-573; discussion 574.Tudorache I, Cebotari S, Sturz G, Kirsch L, Hurschler C, Hilfiker A et al. Tissue Engineering of Heart Valves: Biomechanical and Morphological Properties of Decellularized Heart Valves. J Heart Valve Dis. 2007 Sep; 16 (5): 567-573; discussion 574.Gilpin A, Yang Y. Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications. Biomed Res Int. 2017 Apr; 2017: 9831534. https://doi.org/10.1155/2017/9831534.Gilpin A, Yang Y. Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications. Biomed Res Int. 2017 Apr; 2017: 9831534. https://doi.org/10.1155/2017/9831534.Steinhoff G, Stock U, Karim N, Mertsching H, Timke A, Meliss RR. Tissue Engineering of Pulmonary Heart Valves on Allogenic Acellular Matrix Conduits: In Vivo Restoration of Valve Tissue. Circulation. 2000 Nov 7; 102 (19 Suppl 3): III50-5. doi: 10.1161/01.cir.102.suppl_3.iii-50.Steinhoff G, Stock U, Karim N, Mertsching H, Timke A, Meliss RR. Tissue Engineering of Pulmonary Heart Valves on Allogenic Acellular Matrix Conduits: In Vivo Restoration of Valve Tissue. Circulation. 2000 Nov 7; 102 (19 Suppl 3): III50-5. doi: 10.1161/01.cir.102.suppl_3.iii-50.Xing Q, Yates K, Tahtinen M, Shearier E, Qian Z, Zhao F. Decellularization of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation. Tissue Engineering Part C: Methods. 2014; 21 (1). doi: 10.1089/ten.tec.2013.0666.Xing Q, Yates K, Tahtinen M, Shearier E, Qian Z, Zhao F. Decellularization of Fibroblast Cell Sheets for Natural Extracellular Matrix Scaffold Preparation. Tissue Engineering Part C: Methods. 2014; 21 (1). doi: 10.1089/ten.tec.2013.0666.Wilson GJ, Courtman DW, Klement P, Lee JM, Yeger H. Acellular matrix: a biomaterials approach for coronary artery bypass and heart valve replacement. Ann Thorac Surg. 1995; 60 (2 Suppl): S353-S358. doi: 10.1016/0003-4975(95)98967-y.Wilson GJ, Courtman DW, Klement P, Lee JM, Yeger H. Acellular matrix: a biomaterials approach for coronary artery bypass and heart valve replacement. Ann Thorac Surg. 1995; 60 (2 Suppl): S353-S358. doi: 10.1016/0003-4975(95)98967-y.Wong ML, Griffiths LG. Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization. Acta Biomaterialia. 31 Jan 2014; 10 (5): 1806-1816. doi: 10.1016/j.actbio.2014.01.028.Wong ML, Griffiths LG. Immunogenicity in xenogeneic scaffold generation: antigen removal vs. decellularization. Acta Biomaterialia. 31 Jan 2014; 10 (5): 1806-1816. doi: 10.1016/j.actbio.2014.01.028.Sayk F, Bos I, Schubert U, Wedel T, Sievers H-H. Histopathologic findings in a novel decellularized pulmonary homograft: An autopsy study. Ann Thorac Surg. 2005; 79: 1755-1758. doi: 10.1016/j.athoracsur.2003.11.049.Sayk F, Bos I, Schubert U, Wedel T, Sievers H-H. Histopathologic findings in a novel decellularized pulmonary homograft: An autopsy study. Ann Thorac Surg. 2005; 79: 1755-1758. doi: 10.1016/j.athoracsur.2003.11.049.Kneib C, von Glehn C, Costa F, Costa M, Susin M. Evaluation of humoral immune response to donor HLA after implantation of cellularized versus decellularized human heart valve allografts. Tissue Antigens. 2012; 80: 165174. doi: 10.1111/j.1399-0039.2012.01885.x. Epub 2012 May 25.Kneib C, von Glehn C, Costa F, Costa M, Susin M. Evaluation of humoral immune response to donor HLA after implantation of cellularized versus decellularized human heart valve allografts. Tissue Antigens. 2012; 80: 165174. doi: 10.1111/j.1399-0039.2012.01885.x. Epub 2012 May 25.Goncalves AC, Griffiths LG, Anthony RV, Orton EC. De-cellularization of bovine pericardium for tissue-engineering by targeted removal of xenoantigens. The Journal of Heart Valve Disease. 01 Mar 2005; 14 (2): 212-217.Goncalves AC, Griffiths LG, Anthony RV, Orton EC. De-cellularization of bovine pericardium for tissue-engineering by targeted removal of xenoantigens. The Journal of Heart Valve Disease. 01 Mar 2005; 14 (2): 212-217.Griffiths LG, Choe LH, Reardon KF, Dow SW, Christopher Orton E. Immunoproteomic identification of bovine pericardium xenoantigens. Biomaterials. 2008; 29: 3514-3520. doi: 10.1016/j.biomaterials.2008.05.006.Griffiths LG, Choe LH, Reardon KF, Dow SW, Christopher Orton E. Immunoproteomic identification of bovine pericardium xenoantigens. Biomaterials. 2008; 29: 3514-3520. doi: 10.1016/j.biomaterials.2008.05.006.Syedain ZH, Bradee AR, Kren S, Taylor DA, Tranquillo RT. Decellularized tissue-engineered heart valve leaflets with recellularization potential. Tissue Engineering Part A. 2012; 19: 759-769. doi: 10.1089/ten.TEA.2012.0365.Syedain ZH, Bradee AR, Kren S, Taylor DA, Tranquillo RT. Decellularized tissue-engineered heart valve leaflets with recellularization potential. Tissue Engineering Part A. 2012; 19: 759-769. doi: 10.1089/ten.TEA.2012.0365.Keane TJ, Londono R, Turner NJ, Badylak SF. Consequences of ineffective decellularization of biologic scaffolds on the host response. Biomaterials. 2012; 33: 1771-1781. doi: 10.1016/j.biomaterials.2011.10.054.Keane TJ, Londono R, Turner NJ, Badylak SF. Consequences of ineffective decellularization of biologic scaffolds on the host response. Biomaterials. 2012; 33: 1771-1781. doi: 10.1016/j.biomaterials.2011.10.054.Gilbert TW, Freund JM, Badylak SF. Quantification of DNA in biologic scaffold materials. The Journal of Surgical Research. 2009; 152: 135-139. doi: 10.1016/j.jss.2008.02.013.Gilbert TW, Freund JM, Badylak SF. Quantification of DNA in biologic scaffold materials. The Journal of Surgical Research. 2009; 152: 135-139. doi: 10.1016/j.jss.2008.02.013.Boer U, Lohrenz A, Klingenberg M, Pich A, Haverich A, Wilhelmi M. The effect of detergent-based decellularization procedures on cellular proteins and immunogenicity in equine carotid artery grafts. Biomaterials. 2011; 32: 9730-9737. doi: 10.1016/j.biomaterials.2011.09.015.Boer U, Lohrenz A, Klingenberg M, Pich A, Haverich A, Wilhelmi M. The effect of detergent-based decellularization procedures on cellular proteins and immunogenicity in equine carotid artery grafts. Biomaterials. 2011; 32: 9730-9737. doi: 10.1016/j.biomaterials.2011.09.015.Kasimir MT, Rieder E, Seebacher G, Nigisch A, Dekan B, Wolner E et al. Decellularization does not eliminate thrombogenicity and inflammatory stimulation in tissue-engineered porcine heart valves. J Heart Valve Dis. 2006; 15 (2): 278-286.Kasimir MT, Rieder E, Seebacher G, Nigisch A, Dekan B, Wolner E et al. Decellularization does not eliminate thrombogenicity and inflammatory stimulation in tissue-engineered porcine heart valves. J Heart Valve Dis. 2006; 15 (2): 278-286.VeDepo MC, Detamore MS, Hopkins RA, Converse GL. Recellularization of decellularized heart valves: Progress toward the tissue-engineered heart valve. J Tissue Eng. 2017 Jan-Dec; 8: 2041731417726327. doi: 10.1177/2041731417726327.VeDepo MC, Detamore MS, Hopkins RA, Converse GL. Recellularization of decellularized heart valves: Progress toward the tissue-engineered heart valve. J Tissue Eng. 2017 Jan-Dec; 8: 2041731417726327. doi: 10.1177/2041731417726327.Casali DM, Handleton RM, Shazly T, Matthews MA. A novel supercritical CO2-based decellularization method for maintaining scaffold hydration and mechanical properties. The Journal of Supercritical Fluids. 2018 Jan; 131: 72-81. https://doi.org/10.1016/j.supflu.2017.07.021.Casali DM, Handleton RM, Shazly T, Matthews MA. A novel supercritical CO2-based decellularization method for maintaining scaffold hydration and mechanical properties. The Journal of Supercritical Fluids. 2018 Jan; 131: 72-81. https://doi.org/10.1016/j.supflu.2017.07.021.Lee JW, Fukusaki E, Bamba T. Application of supercritical fluid carbon dioxide to the extraction and analysis of lipids. Bioanalysis. 2012; 4: 2413-2422. https://doi.org/10.4155/bio.12.198.Lee JW, Fukusaki E, Bamba T. Application of supercritical fluid carbon dioxide to the extraction and analysis of lipids. Bioanalysis. 2012; 4: 2413-2422. https://doi.org/10.4155/bio.12.198.Hennessy RS, Jana S, Tefft BJ, Helder MR, Young MD, Hennessy RR et al. Supercritical Carbon Dioxide-Based Sterilization of Decellularized Heart Valves. JACC Basic Transl Sci. 2017 Feb; 2 (1): 71-84. doi: 10.1016/j.jacbts.2016.08.009.Hennessy RS, Jana S, Tefft BJ, Helder MR, Young MD, Hennessy RR et al. Supercritical Carbon Dioxide-Based Sterilization of Decellularized Heart Valves. JACC Basic Transl Sci. 2017 Feb; 2 (1): 71-84. doi: 10.1016/j.jacbts.2016.08.009.

The authors declare that there are no conflicts of interest present.