vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2021-1-75-83vtio-1317Research ArticleРЕГЕНЕРАТИВНАЯ МЕДИЦИНА И КЛЕТОЧНЫЕ ТЕХНОЛОГИИREGENERATIVE MEDICINE AND CELL TECHNOLOGIESОценка резистентности к кальцификации ксеноперикарда, обработанного полигидроксисоединениямиEvaluation of calcification resistance of xenopericardium treated with polyhydroxy compoundsРезвоваМ. А.RezvovaM. A.

Резвова Мария Александровна.

650002, Кемерово, Сосновый бульвар, 6.

Тел. (913) 079-61-40

Maria Rezvova.

6, Sosnovy Boulevard, Kemerovo, 650002.

Phone: (913) 079-61-40

rezvovama@gmail.comОвчаренкоЕ. А.OvcharenkoE. A.

650002, Кемерово, Сосновый бульвар, 6.

6, Sosnovy Boulevard, Kemerovo, 650002.

ГлушковаТ. В.GlushkovaT. V.

650002, Кемерово, Сосновый бульвар, 6.

6, Sosnovy Boulevard, Kemerovo, 650002.

КудрявцеваЮ. A.KudryavtsevaYu. A.

650002, Кемерово, Сосновый бульвар, 6.

6, Sosnovy Boulevard, Kemerovo, 650002.

БарбарашЛ. С.BarbarashL. S.

650002, Кемерово, Сосновый бульвар, 6.

6, Sosnovy Boulevard, Kemerovo, 650002.

Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеванийРоссияResearch Institute for Complex Issues of Cardiovascular DiseasesRussian Federation2021100420212317583Copyright © Резвова М.А., Овчаренко Е.А., Глушкова Т.В., Кудрявцева Ю.A., Барбараш Л.С., 20212021Резвова М.А., Овчаренко Е.А., Глушкова Т.В., Кудрявцева Ю.A., Барбараш Л.С.Rezvova M.A., Ovcharenko E.A., Glushkova T.V., Kudryavtseva Y.A., Barbarash L.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1317

Кальцификация биологического материала, используемого при протезировании клапанов сердца, является актуальной проблемой сердечно-сосудистой хирургии. Цель настоящей работы - сравнительная оценка эффективности модифицирования поливиниловым спиртом (ПВС) и дубильной кислотой (ДК) ксеноткани, предварительно стабилизированной глутаровым альдегидом (ГА) и диглицидиловым эфиром этиленгликоля (ДЭЭ), в снижении уровня кальцификации. Анализ механических свойств, оцененный в условиях одноосного растяжения, продемонстрировал значимое увеличение предела прочности на разрыв исследуемых образцов по сравнению с тем же показателем для контрольных (немодифицированных) образцов (p < 0,05). Дополнительная обработка ГА-фиксированной ткани ПВС и ДК позволила добиться значимого снижения количества кальция в образцах, имплантированных крысам на срок наблюдения 60 дней (p < 0,05). Уровень кальцификации образцов, предварительно стабилизированных ДЭЭ и обработанных ПВС и ДК, не отличался от контрольной группы (p = 0,063). Совокупный анализ результатов исследования продемонстрировал способность биоматериала, предварительно консервированного ГА и дополнительно модифицированного ПВС и ДК, к снижению кальций-связывающей активности и повышению прочности, что свидетельствует о перспективах клинического применения предложенных способов обработки. В то же время вопрос отдаленной реакции организма требует дальнейшего изучения долгосрочной стабильности модифицированного биоматериала в условиях физиологического кровотока.

Calcification of biomaterials used in prosthetic heart valves has been a challenging issue in cardiovascular surgery. The objective of this work is to compare the efficiency of polyvinyl alcohol (PVA) and tannic acid (TA) modification of xenomaterials, pre-stabilized with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (EGDE), in reducing calcification. Analysis of mechanical properties evaluated under uniaxial tension, showed a significant increase in the tensile strength of the test samples compared to the control (unmodified) samples (p < 0.05). Additional treatment of GA-fixed tissue with PVA and TA significantly reduced the amount of calcium in the samples implanted into rats for a 60-day follow-up (p < 0.05). The level of calcification of samples prestabilized with EGDE and treated with PVA and TA did not differ from the control group (p = 0.063). Cumulative analysis of the study results demonstrated that the GA-fixed biomaterial modified with PVA and TA can reduce calcium-binding activity and increase strength. This indicates the prospects for clinical application of the proposed treatment methods. This being said, the issue of long-term body response requires further study of the long-term stability of the modified biomaterial under physiologic blood flow conditions.

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The authors declare that there are no conflicts of interest present.