vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2020-4-105-114vtio-1271Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesБиодеградируемые материалы на основе тканей из натурального шелка как перспективные скаффолды для тканевой инженерии и регенеративной медициныBiodegradable materials based on natural silk fabric as promising scaffolds for tissue engineering and regenerative medicineСафоноваЛ. А.SafonovaL. A.

Москва

Moscow

БоброваМ. М.BobrovaM. M.

Москва

Moscow

ЕфимовА. Е.EfimovA. E.

Москва

Moscow

АгаповаО. И.AgapovaO. I.

Москва

Moscow

АгаповИ. И.AgapovI. I.

123182, Москва, ул. Щукинская, д. 1

Тел. (985) 231-60-42

1, Schukinskaya str., Moscow, 123182, Russian Federation

Phone: (985) 231-60-42

igor_agapov@mail.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian Federation202026012021224105114Copyright © Сафонова Л.А., Боброва М.М., Ефимов А.Е., Агапова О.И., Агапов И.И., 20212021Сафонова Л.А., Боброва М.М., Ефимов А.Е., Агапова О.И., Агапов И.И.Safonova L.A., Bobrova M.M., Efimov A.E., Agapova O.I., Agapov I.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1271Objective

Objective: to develop a method for obtaining scaffolds based on natural silk fabric and to study their biocompatibility in vitro.

Materials and methods

Materials and methods. To obtain biodegradable scaffolds based on natural silk fabric, we propose treating natural silk fabric with a water-ethanol solution of calcium chloride. Differences in the structure of the resulting scaffolds were identified via scanning electron microscopy.

Conclusion

Conclusion. The resulting scaffolds are non-toxic to cells and support cell adhesion and proliferation. Our studies make it possible to consider the resulting biodegradable scaffolds as promising constructs for tissue engineering and regenerative medicine.

шелкткань из натурального шелкабиодеградируемые скаффолдыскорость биодеградацииsilknatural silk fabricbiodegradable scaffoldsbiodegradation rateReferencesKoh L-D, Cheng Y, Teng C-P, Khin Y-W, Loh X-J, Tee S-Y et al. Structures, mechanical properties and applications of silk fibroin materials. Progress in Polymer Science. 2015; 46: 86–110. doi: 10.1016/j.progpolymsci.2015.02.001.Koh L-D, Cheng Y, Teng C-P, Khin Y-W, Loh X-J, Tee S-Y et al. Structures, mechanical properties and applications of silk fibroin materials. Progress in Polymer Science. 2015; 46: 86–110. doi: 10.1016/j.progpolymsci.2015.02.001.Ding Z, Han H, Fan Z, Lu H, Sang Y, Yao Y et al. Nanoscale Silk–Hydroxyapatite Hydrogels for Injectable Bone Biomaterials. ACS applied materials & interfaces. 2017; 9: 16913–16921. doi: 10.1021/acsami.7b03932. PMID: 28471165.Ding Z, Han H, Fan Z, Lu H, Sang Y, Yao Y et al. Nanoscale Silk–Hydroxyapatite Hydrogels for Injectable Bone Biomaterials. ACS applied materials & interfaces. 2017; 9: 16913–16921. doi: 10.1021/acsami.7b03932. PMID: 28471165.Kim DK, Sim BR, Khang G. Nature-derived aloe vera gel blended silk fibroin film scaffolds for cornea endothelial cell regeneration and transplantation. ACS applied materials & interfaces. 2016; 8: 15160–15168. doi: 10.1021/acsami.6b04901. PMID: 27243449.Kim DK, Sim BR, Khang G. Nature-derived aloe vera gel blended silk fibroin film scaffolds for cornea endothelial cell regeneration and transplantation. ACS applied materials & interfaces. 2016; 8: 15160–15168. doi: 10.1021/acsami.6b04901. PMID: 27243449.Dinis T, Elia R, Vidal G, Dermigny Q, Denoeud C, Kaplan D et al. 3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration. Journal of the mechanical behavior of biomedical materials. 2015; 41: 43–55. doi: 10.1016/j.jmbbm.2014.09.029. PMID: 25460402.Dinis T, Elia R, Vidal G, Dermigny Q, Denoeud C, Kaplan D et al. 3D multi-channel bi-functionalized silk electrospun conduits for peripheral nerve regeneration. Journal of the mechanical behavior of biomedical materials. 2015; 41: 43–55. doi: 10.1016/j.jmbbm.2014.09.029. PMID: 25460402.Wang Y, Rudym DD, Walsh A, Abrahamsen L, Kim H-J, Kim HS et al. In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials. 2008; 29: 3415–3428. doi: 10.1016/j.biomaterials.2008.05.002. PMID: 18502501.Wang Y, Rudym DD, Walsh A, Abrahamsen L, Kim H-J, Kim HS et al. In vivo degradation of three-dimensional silk fibroin scaffolds. Biomaterials. 2008; 29: 3415–3428. doi: 10.1016/j.biomaterials.2008.05.002. PMID: 18502501.Gupta P, Lorentz KL, Haskett DG, Cunnane EM, Ramaswamy AK, Weinbaum JS et al. Bioresorbable silk grafts for small diameter vascular tissue engineering applications: In vitro and in vivo functional analysis. Acta Biomater. 2020; 15 (105): 146–158. doi: 10.1016/j.actbio.2020.01.020. PMID: 31958596.Gupta P, Lorentz KL, Haskett DG, Cunnane EM, Ramaswamy AK, Weinbaum JS et al. Bioresorbable silk grafts for small diameter vascular tissue engineering applications: In vitro and in vivo functional analysis. Acta Biomater. 2020; 15 (105): 146–158. doi: 10.1016/j.actbio.2020.01.020. PMID: 31958596.Hasturk O, Jordan KE, Choi J, Kaplan DL. Enzymatically crosslinked silk and silk-gelatin hydrogels with tunable gelation kinetics, mechanical properties and bioactivity for cell culture and encapsulation. Biomaterials. 2019; 232: 119720. doi: 10.1016/j.biomaterials.2019.119720. PMID: 31896515.Hasturk O, Jordan KE, Choi J, Kaplan DL. Enzymatically crosslinked silk and silk-gelatin hydrogels with tunable gelation kinetics, mechanical properties and bioactivity for cell culture and encapsulation. Biomaterials. 2019; 232: 119720. doi: 10.1016/j.biomaterials.2019.119720. PMID: 31896515.Wang Y, Fan S, Li Y, Niu C, Li X, Guo Y et al. Silk fibroin/sodium alginate composite porous materials with controllable degradation. Int J Biol Macromol. 2019; 1 (150): 1314–1322. doi: 10.1016/j.ijbiomac.2019.10.141. PMID: 31747567.Wang Y, Fan S, Li Y, Niu C, Li X, Guo Y et al. Silk fibroin/sodium alginate composite porous materials with controllable degradation. Int J Biol Macromol. 2019; 1 (150): 1314–1322. doi: 10.1016/j.ijbiomac.2019.10.141. PMID: 31747567.Ratanavaraporn J, Kanokpanont S, Damrongsakkul S. The development of injectable gelatin/silk fibroin microspheres for the dual delivery of curcumin and piperine. J Mater Sci Mater Med. 2014; 25 (2): 401–410. doi: 10.1007/s10856-013-5082-3. PMID: 24186150.Ratanavaraporn J, Kanokpanont S, Damrongsakkul S. The development of injectable gelatin/silk fibroin microspheres for the dual delivery of curcumin and piperine. J Mater Sci Mater Med. 2014; 25 (2): 401–410. doi: 10.1007/s10856-013-5082-3. PMID: 24186150.Yang W, Xu H, Lan Y, Zhu Q, Liu Y, Huang S et al. Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair. Int J Biol Macromol. 2019; 1 (130): 58–67. doi: 10.1016/j.ijbiomac.2019.02.120. PMID: 30797808.Yang W, Xu H, Lan Y, Zhu Q, Liu Y, Huang S et al. Preparation and characterisation of a novel silk fibroin/hyaluronic acid/sodium alginate scaffold for skin repair. Int J Biol Macromol. 2019; 1 (130): 58–67. doi: 10.1016/j.ijbiomac.2019.02.120. PMID: 30797808.Marcolin C, Draghi L, Tanzi M, Faré S. Electrospun silk fibroin-gelatin composite tubular matrices as scaffolds for small diameter blood vessel regeneration. J Mater Sci Mater Med. 2017; 28 (5): 80. doi: 10.1007/s10856-017-5884-9. PMID: 28397163.Marcolin C, Draghi L, Tanzi M, Faré S. Electrospun silk fibroin-gelatin composite tubular matrices as scaffolds for small diameter blood vessel regeneration. J Mater Sci Mater Med. 2017; 28 (5): 80. doi: 10.1007/s10856-017-5884-9. PMID: 28397163.Сафонова ЛА, Боброва ММ, Агапова ОИ, Архипова АЮ, Гончаренко АВ, Агапов ИИ. Пленки на основе фиброина шелка для заживления полнослойной раны кожи у крыс. Вестник трансплантологии и искусственных органов. 2016; 18 (3): 80–83. doi: 10.15825/1995-1191-2016-3-74-84.Safonova LA, Bobrova MM, Agapova OI, Arkhipova AYu, Goncharenko AV, Agapov II. Fibroin Silk Based Films For Rat’s Full-Thickness Skin Wound Regeneration. Russian Journal of Transplantology and Artificial Organs. 2016; 18 (3): 74–84. [In Russ, English abstract]. doi: 10.15825/1995-1191-2016-3-74-84.Li T, Song X, Weng C, Wang X, Gu L, Gong X et al. Silk fibroin/carboxymethyl chitosan hydrogel with tunable biomechanical properties has application potential as cartilage scaffold. Int J Biol Macromol. 2019; 137: 382–391. doi: 10.1016/j.ijbiomac.2019.06.245. PMID: 31271796.Li T, Song X, Weng C, Wang X, Gu L, Gong X et al. Silk fibroin/carboxymethyl chitosan hydrogel with tunable biomechanical properties has application potential as cartilage scaffold. Int J Biol Macromol. 2019; 137: 382–391. doi: 10.1016/j.ijbiomac.2019.06.245. PMID: 31271796.Wang F, Wu H, Venkataraman V, Hu X. Silk fibroinpoly(lactic acid) biocomposites: Effect of protein-synthetic polymer interactions and miscibility on material properties and biological responses. Mater Sci Eng C Mater Biol Appl. 2019; 104: 109890. doi: 10.1016/j.msec.2019.109890. PMID: 31500018.Wang F, Wu H, Venkataraman V, Hu X. Silk fibroinpoly(lactic acid) biocomposites: Effect of protein-synthetic polymer interactions and miscibility on material properties and biological responses. Mater Sci Eng C Mater Biol Appl. 2019; 104: 109890. doi: 10.1016/j.msec.2019.109890. PMID: 31500018.Roy T, Maity PP, Rameshbabu AP, Das B, John A, Dutta A et al. Core-Shell Nanofibrous Scaffold Based on Polycaprolactone-Silk Fibroin Emulsion Electrospinning for Tissue Engineering Applications. Bioengineering (Basel). 2018; 5 (3): 68. doi: 10.3390/bioengineering5030068. PMID: 30134543.Roy T, Maity PP, Rameshbabu AP, Das B, John A, Dutta A et al. Core-Shell Nanofibrous Scaffold Based on Polycaprolactone-Silk Fibroin Emulsion Electrospinning for Tissue Engineering Applications. Bioengineering (Basel). 2018; 5 (3): 68. doi: 10.3390/bioengineering5030068. PMID: 30134543.Zhou F, Zhang X, Cai D, Li J, Mu Q, Zhang W et al. Silk fibroin-chondroitin sulfate scaffold with immuno-inhibition property for articular cartilage repair. Acta Biomater. 2017; 63: 64–75. doi: 10.1016/j.actbio.2017.09.005. PMID: 28890259.Zhou F, Zhang X, Cai D, Li J, Mu Q, Zhang W et al. Silk fibroin-chondroitin sulfate scaffold with immuno-inhibition property for articular cartilage repair. Acta Biomater. 2017; 63: 64–75. doi: 10.1016/j.actbio.2017.09.005. PMID: 28890259.Shen Y, Tu T, Yi B, Wang X, Tang H, Liu W, Zhang Y. Electrospun acid-neutralizing fibers for the amelioration of inflammatory response. Acta Biomater. 2019; 97: 200–215. doi: 10.1016/j.actbio.2019.08.014. PMID: 31400522.Shen Y, Tu T, Yi B, Wang X, Tang H, Liu W, Zhang Y. Electrospun acid-neutralizing fibers for the amelioration of inflammatory response. Acta Biomater. 2019; 97: 200–215. doi: 10.1016/j.actbio.2019.08.014. PMID: 31400522.Агапов ИИ, Агапова ОИ, Ефимов АЕ, Соколов ДЮ, Боброва ММ, Сафонова ЛА. Способ получения биодеградируемых скаффолдов на основе тканей из натурального шелка. Патент на изобретение RU2653428 С1, 08.05.2018.Agapov II, Agapova OI, Efimov AE, Sokolov DYu, Bobrova MM, Safonova LA. Sposob polucheniya biodegradiruemykh skaffoldov na osnove tkaney iz natural’nogo shelka. Patent na izobretenie RU2653428 S1, 08.05.2018.Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J lmmunol Methods. 1983; 65 (1–2): 55–63. doi: 10.1016/0022-1759(83)90303-4. PMID: 6606682.Mosmann T. Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays. J lmmunol Methods. 1983; 65 (1–2): 55–63. doi: 10.1016/0022-1759(83)90303-4. PMID: 6606682.Shen T, Wang T, Cheng G, Huang L, Chen L, Wu D. Dissolution behavior of silk fibroin in a low concentration CaCl2-methanol solvent: From morphology to nanostructure. Int J Biol Macromol. 2018; 1 (113): 458–463. doi: 10.1016/j.ijbiomac.2018.02.022. PMID: 29421494.Shen T, Wang T, Cheng G, Huang L, Chen L, Wu D. Dissolution behavior of silk fibroin in a low concentration CaCl2-methanol solvent: From morphology to nanostructure. Int J Biol Macromol. 2018; 1 (113): 458–463. doi: 10.1016/j.ijbiomac.2018.02.022. PMID: 29421494.Hino T, Tanimoto M, Shimabayashi S. Change in secondary structure of silk fibroin during preparation of its microspheres by spray-drying and exposure to humid atmosphere. J Colloid Interface Sci. 2003; 266 (1): 68–73. doi: 10.1016/s0021-9797(03)00584-8. PMID: 12957583.Hino T, Tanimoto M, Shimabayashi S. Change in secondary structure of silk fibroin during preparation of its microspheres by spray-drying and exposure to humid atmosphere. J Colloid Interface Sci. 2003; 266 (1): 68–73. doi: 10.1016/s0021-9797(03)00584-8. PMID: 12957583.HuY,ZhangQ,YouR,WangL,LiM.The Relationship between Secondary Structure and Biodegradation Behavior of Silk Fibroin Scaffolds. Adv Mater Sci and Eng. 2012; 2012: 21–25. https://doi.org/10.1155/2012/185905.HuY,ZhangQ,YouR,WangL,LiM.The Relationship between Secondary Structure and Biodegradation Behavior of Silk Fibroin Scaffolds. Adv Mater Sci and Eng. 2012; 2012: 21–25. https://doi.org/10.1155/2012/185905.Ефимов АЕ, Агапова ОИ, Сафонова ЛА, Боброва ММ, Агапов ИИ. Трехмерный анализ микро- и наноструктуры биоматериалов и клеток методом сканирующей зондовой крионанотомографии. Вестник трансплантологии и искусственных органов. 2017; 19 (4): 78–87. doi: 10.15825/1995-1191-2017-4-78-87.Efimov AE, Agapova OI, Safonova LA, Bobrova MM, Agapov II. Three-dimensional analysis of micro and nanostructure of biomaterials and cells by method of scanning probe nanotomography. Russian Journal of Transplantology and Artificial Organs. 2017; 19 (4): 78–87. doi: 10.15825/1995-1191-2017-4-78-87.Zhang F, Xu S, Wang Z. Pre-treatment optimization and properties of gelatin from freshwater fish scales. Food Bioprod Proc. 2011; 89: 185–193. doi: 10.1016/j.fbp.2010.05.003.Zhang F, Xu S, Wang Z. Pre-treatment optimization and properties of gelatin from freshwater fish scales. Food Bioprod Proc. 2011; 89: 185–193. doi: 10.1016/j.fbp.2010.05.003.Su K., Wang C. Recent advances in the use of gelatin in biomedical research. Biotechnol Lett. 2015; 37 (11): 2139–2145. doi: 10.1007/s10529-015-1907-0. PMID: 26160110.Su K., Wang C. Recent advances in the use of gelatin in biomedical research. Biotechnol Lett. 2015; 37 (11): 2139–2145. doi: 10.1007/s10529-015-1907-0. PMID: 26160110.Abdulghani S, Mitchell GR. Biomaterials for In Situ Tissue Regeneration: A Review. Biomolecules. 2019; 9 (11): 750. doi: 10.3390/biom9110750. PMID: 31752393; PMCID: PMC6920773.Abdulghani S, Mitchell GR. Biomaterials for In Situ Tissue Regeneration: A Review. Biomolecules. 2019; 9 (11): 750. doi: 10.3390/biom9110750. PMID: 31752393; PMCID: PMC6920773.

The authors declare that there are no conflicts of interest present.