vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2020-3-143-148vtio-1237Research ArticleРегенеративная медицина и клеточные технологииRegenerative Medicine and Cell TechnologiesТрехмерный анализ микро- и наноструктуры ткани легкого методом сканирующей зондовой нанотомографии3D analysis of the micro- and nanostructure of lung tissue by scanning probe nanotomographyЕфимовА. Е.EfimovA. E.

Москва

Moscow

АгаповаО. И.AgapovaO. I.

Москва

Moscow

СафоноваЛ. А.SafonovaL. A.

Москва

Moscow

БоброваМ. М.BobrovaM. M.

Москва

Moscow

АгаповИ. И.AgapovI. I.

Агапов Игорь Иванович.

Адрес: 123182, Москва, ул. Щукинская, д. 1.

Тел. (499) 190-66-19.

Igor Agapov.

Address: 1, Shchukinskaya str., Moscow, 123182, Russian Fedeartion. 

Phone: (499) 190-66-19.

igor_agapov@mail.ruФГБУ «Национальный медицинский исследовательский центр трансплантологии и искусственных органов имени академика В.И. Шумакова» Минздрава РоссииРоссияShumakov National Medical Research Center of Transplantology and Artificial OrgansRussian Federation202006102020223143148Copyright © Ефимов А.Е., Агапова О.И., Сафонова Л.А., Боброва М.М., Агапов И.И., 20202020Ефимов А.Е., Агапова О.И., Сафонова Л.А., Боброва М.М., Агапов И.И.Efimov A.E., Agapova O.I., Safonova L.A., Bobrova M.M., Agapov I.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1237

Цель. Провести анализ трехмерной микро- и наноструктуры и количественных морфологических параметров ткани легкого крысы. Материалы и методы. Для исследования, были получены образцы ткани легкого крысы породы Wistar. Изучение трехмерной структуры ткани легкого было выполнено методом сканирующей зондовой нанотомографии при помощи экспериментальной установки, объединяющей ультрамикротом и сканирующий зондовый микроскоп. Результаты. Получены наномасштабные изображения и трехмерные нанотомографические реконструкции участков межальвеолярных перегородок легкого крысы. Определены морфологические параметры поверхности межальвеолярной перегородки: средняя шероховатость, удельная эффективная площадь. Установлено, что средняя шероховатость реконструированной поверхности перегородкисоставляет 345,4 ± 24,5 нм, а удельная эффективная площадь 2,7 ± 0,2 ед. Выводы. Полученные в результате исследования данные демонстрируют, что технология сканирующей зондовой нанотомографии позволяет определять количественные характеристики морфологии ткани легкого. Использование метода сканирующей зондовой нанотомографии для трехмерного анализа структуры и характеристик ткани легкого позволит повысить эффективность разработок по созданию новых критериев диагностики патологических состояний.

Objective: to analyze the 3D micro- and nanostructure and quantitative morphological parameters of rat lung tissue. Materials and methods. Wistar rat lung tissue samples were obtained for the study. The 3D structure of the lung tissue was studied via scanning probe nanotomography using an experimental setup combining an ultramicrotome and a scanning probe microscope. Results. Nanoscale images and 3D nanotomographic reconstructions of the interalveolar septal sections of the rat lung were obtained. Morphological parameters (average roughness and specific effective area) of the interalveolar septal surface were determined. It was found that the average roughness of the reconstructed septal surface was 345.4 ± 24.5 nm, and the specific effective area was 2.7 ± 0.2 units. Conclusions. Results obtained demonstrate that scanning probe nanotomography allows to quantify lung morphology. The use of scanning probe nanotomography for 3D analysis of the structure and characteristics of lung tissue will increase the efficiency of future developments on creation of new criteria for diagnosing pathological conditions.

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The authors declare that there are no conflicts of interest present.