vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2021-3-115-121vtio-1217Research ArticleРЕГЕНЕРАТИВНАЯ МЕДИЦИНА И КЛЕТОЧНЫЕ ТЕХНОЛОГИИREGENERATIVE MEDICINE AND CELL TECHNOLOGIESЭкспрессия тканевых ингибиторов металлопротеиназ типа 1 и 2 в створках эксплантированных биопротезов клапанов сердца – новая патогенетическая параллель между структурной дегенерацией клапана и кальцинирующим аортальным стенозомExpression of tissue inhibitors of metalloproteinases type 1 and type 2 in the leaflets of explanted bioprosthetic heart valves: a new pathogenetic parallel between structural valve degeneration and calcific aortic stenosisКостюнинА. Е.KostyuninA. E.

Костюнин Александр Евгеньевич, научный сотрудник Лаборатории новых биоматериалов

650002, Кемерово, Сосновый бульвар, 6

Alexander E. Kostyunin, Researcher, Laboratory of New Biomaterials

6, Sosnovy Boulevard, Kemerovo, 650002

Rhabdophis_tigrina@mail.ruГлушковаТ. В.GlushkovaT. V.

Кемерово

Kemerovo

БогдановЛ. А.BogdanovL. A.

Кемерово

Kemerovo

ОвчаренкоЕ. А.OvcharenkoE. A.

Кемерово

Kemerovo

ФГБНУ «Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний»РоссияResearch Institute for Complex Issues of Cardiovascular DiseasesRussian Federation202117092021233115121Copyright © Костюнин А.Е., Глушкова Т.В., Богданов Л.А., Овчаренко Е.А., 20212021Костюнин А.Е., Глушкова Т.В., Богданов Л.А., Овчаренко Е.А.Kostyunin A.E., Glushkova T.V., Bogdanov L.A., Ovcharenko E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1217Цель

Цель: изучить клеточную и липидную инфильтрацию, а также экспрессию тканевых ингибиторов металлопротеиназ (ТИМП) типа 1 и 2 в створках биологических протезов клапанов сердца (БПК), эксплантированных по причине дисфункций.

Материал и методы

Материал и методы. Исследовано 17 створок от 6 БПК, иссеченных из аортальной и митральной позиций при репротезировании клапанов. Для выполнения микроскопического анализа фрагменты створок БПК замораживали и с помощью криотома изготавливали серийные срезы. С целью изучения клеточной инфильтрации и степени дегенеративных изменений протезного биоматериала срезы окрашивали гематоксилином Джилла и эозином, для оценки отложения липидов использовали окраску Oil Red. С целью типирования клеток и выявления ТИМП-1/-2 применяли иммуногистохимический метод. Анализ окрашенных образцов осуществляли методом световой микроскопии.

Результаты

Результаты. Клеточная и липидная инфильтрация ксенотканей выявлена во всех исследованных створках БПК. Клетки реципиентов коэкспрессировали панлейкоцитарный и макрофагальный маркеры PTPRC/CD45 и CD68. Положительная окраска на ТИМП-1/-2 солокализовалась с клеточными скоплениями, но отсутствовала в бесклеточных участках.

Заключение

Заключение. Клетки, проникающие в ксеногенные ткани БПК, экспрессируют ТИМП-1/-2. Это указывает на частичное сходство патофизиологии иммунного отторжения БПК с таковой кальцинирующего аортального стеноза.

Objective

Objective: to study cellular and lipid infiltration, as well as the expression of tissue inhibitors of metalloproteinases (TIMP) types 1 and 2 in biological prosthetic heart valves (BPHVs) explanted due to dysfunction.

Material and Methods

Material and Methods. We examined 17 leaflets from 6 BPHVs, dissected from the aortic and mitral positions during valve replacement. For microscopic analysis, fragments of the BPHV leaflets were frozen and serial sections were made using a cryotome. In order to study cellular infiltration and the degree of degenerative changes in the prosthetic biomaterial, the sections were stained with Gill’s hematoxylin and eosin; Oil Red O stain was used to assess lipid deposition. Immunohistochemistry was used for cell typing and detection of TIMP-1/-2. The stained samples were analyzed by light microscopy.

Results

Results. Cellular and lipid infiltration of xenogeneic tissues was detected in all BPHV flaps studied. Recipient cells coexpressed pan-leukocyte and macrophage markers PTPRC/CD45 and CD68. Positive staining for TIMP-1/-2 co-localized with cell clusters but was absent in acellular sections.

Conclusion

Conclusion. Cells infiltrating xenogeneic BPHV tissues express TIMP-1/-2. This suggests that BPHV immune rejection pathophysiology is partially similar to that of calcific aortic stenosis.

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The authors declare that there are no conflicts of interest present.