vtioВестник трансплантологии и искусственных органовRussian Journal of Transplantology and Artificial Organs1995-1191Academician V.I.Shumakov National Medical Research Center of Transplantology and Artificial Organs", Ministry of Health of the Russian Federation10.15825/1995-1191-2019-4-67-80vtio-1108Research ArticleРЕГЕНЕРАТИВНАЯ МЕДИЦИНА И КЛЕТОЧНЫЕ ТЕХНОЛОГИИREGENERATIVE MEDICINE AND CELL TECHNOLOGIESПерспективы использования триблок-сополимеров SIBS в кардиохирургии: in vitro и in vivo исследование в сравнении с ePTFESIBS triblock copolymers in cardiac surgery: in vitro and in vivo studies in comparison with ePTFEРезвоваМ. А.RezvovaM. A.

Резвова Мария Александровна

650002, г. Кемерово, Сосновый бульвар, 6.Тел. (913) 079-61-40.

Rezvova Maria Alexandrovna

6, Sosnoviy blvd, 650002, Kemerovo.Tel. (913) 079-61-40

rezvovamaria@mail.ruОвчаренкоЕ. А.OvcharenkoE. A.

Кемерово

Kemerovo

НикишевП. А.NikishevP. A.

Минск

Minsk

КостюкС. В.KostyukS. V.

Минск

Москва

Minsk

Moscow

АнтоноваЛ. В.AntonovaL. V.

Кемерово

Kemerovo

АкентьеваТ. Н.Akent’evaT. N.

Кемерово

Kemerovo

ГлушковаТ. В.GlushkovaT. V.

Кемерово

Kemerovo

ВеликановаЕ. Г.VelikanovaY. G.

Кемерово

Kemerovo

ШишковаД. К.ShishkovaD. K.

Кемерово

Kemerovo

КривкинаЕ. О.KrivkinaE. O.

Кемерово

Kemerovo

КлышниковК. Ю.KlyshnikovK. Yu.

Кемерово

Kemerovo

КудрявцеваЮ. А.KudryavtsevaYu. A.

Кемерово

Kemerovo

БарбарашЛ. С.BarbarashL. S.

Кемерово

Kemerovo

ФГБНУ «Научно-исследовательский институт комплексных проблем сердечно-сосудистых заболеваний»РоссияResearch Institute for Complex Issues of Cardiovascular DiseasesRussian FederationНаучно-исследовательский институт физико-химических проблем Белорусского государственного университета; Химический факультет Белорусского государственного университетаБеларусьResearch Institute for Physical Chemical Problems; Faculty of Chemistry, Belarusian State UniversityBelarusНаучно-исследовательский институт физико-химических проблем Белорусского государственного университета; Химический факультет Белорусского государственного университета; Институт регенеративной медицины, ФГАОУ ВО «Первый Московский государственный медицинский университет имени И.М. Сеченова» Минздрава России (Сеченовский университет)БеларусьResearch Institute for Physical Chemical Problems; Faculty of Chemistry, Belarusian State University; Institute of Regenerative Medicine, Sechenov First Moscow State Medical UniversityBelarus2019010220202146780Copyright © Резвова М.А., Овчаренко Е.А., Никишев П.А., Костюк С.В., Антонова Л.В., Акентьева Т.Н., Глушкова Т.В., Великанова Е.Г., Шишкова Д.К., Кривкина Е.О., Клышников К.Ю., Кудрявцева Ю.А., Барбараш Л.С., 20202020Резвова М.А., Овчаренко Е.А., Никишев П.А., Костюк С.В., Антонова Л.В., Акентьева Т.Н., Глушкова Т.В., Великанова Е.Г., Шишкова Д.К., Кривкина Е.О., Клышников К.Ю., Кудрявцева Ю.А., Барбараш Л.С.Rezvova M.A., Ovcharenko E.A., Nikishev P.A., Kostyuk S.V., Antonova L.V., Akent’eva T.N., Glushkova T.V., Velikanova Y.G., Shishkova D.K., Krivkina E.O., Klyshnikov K.Y., Kudryavtseva Y.A., Barbarash L.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.transpl.ru/vtio/article/view/1108

Протезирование клапанов сердца полимерными конструкциями лепесткового типа в перспективе позволит решить проблемы существующих клапанных заменителей – механических и биологических. Целью настоящей работы является комплексная оценка свойств гемосовместимости полимерных материалов на основе синтезированного методом контролируемой катионной полимеризации триблок-сополимера стирола и изобутилена (SIBS) в сравнении с использующимся в клинической практике политетрафторэтиленом (ePTFE). Материалы и методы. Пленки на основе SIBS изготавливали методом полива из раствора полимера; оценку биосовместимости in vitro проводили с использованием клеточных культур, определяя жизнеспособность клеток, клеточную адгезию и пролиферацию; склонность материалов к кальцификации определяли методом ускоренной кальцификации in vitro; оценку биосовместимости in vivo проводили путем подкожной имплантации образцов крысам; гемосовместимость определяли ex vivo по результатам оценки степени гемолиза, агрегации и адгезии тромбоцитов. Результаты. Молекулярная масса синтезированного полимера составила 33 000 г/моль с показателем полидисперсности 1,3. При изучении клеточной адгезии не выявлено достоверных отличий (p = 0,20) свойств полимера SIBS (588 кл/мм2 ) от свойств культурального пластика (732 кл/мм2 ), адгезия клеток для материала ePTFE составила 212 кл/мм2 . Процент мертвых клеток на образцах SIBS, ePTFE составил соответственно 4,40 и 4,72% (p = 0,93), для культурального пластика – 1,16% (p < 0,05). Пролиферация клеток на поверхности ePTFE (0,10%) оказалась значимо ниже (p < 0,05) тех же параметров для SIBS и культурального пластика (62,04 и 44,00%). Результаты имплантации (60 дней) показали формирование фиброзных капсул со средними толщинами 42 мкм (ePTFE) и 58 мкм (SIBS). Содержание кальция в эксплантированных образцах составило: 0,39 мг/г (SIBS), 1,25 мг/г (ePTFE) и 93,79 мг/г (GA-ксеноперикард) (p < 0,05). Уровень гемолиза эритроцитов крови после контакта с SIBS составил 0,35%, еPTFE – 0,40%, что ниже положительного контроля (р < 0,05). Максимум агрегации тромбоцитов интактной обогащенной тромбоцитами плазмы крови составил 8,60%, контактировавшей с полимером SIBS – 18,11%, еPTFE – 22,74%. Заключение. Исследуемый полимер SIBS при сравнении свойств гемосовместимости не уступает ePTFE и может быть использован в качестве основы при разработке полимерного протеза клапана сердца.

Implantation of polymeric heart valves can solve the problems of existing valve substitutes – mechanical and biological. Objective: to comprehensively assess the hemocompatibility of styrene-isobutylene-styrene (SIBS) triblock copolymer, synthesized by controlled cationic polymerization in comparison with expanded polytetrafluoroethylene (ePTFE) used in clinical practice. Materials and methods. SIBS-based films were made by polymer solution casting method; in vitro biocompatibility assessment was performed using cell cultures, determining cell viability, cell adhesion and proliferation; tendency of materials to calcify was determined through in vitro accelerated calcification; in vivo biocompatibility assessment was performed by subcutaneous implantation of rat samples; hemocompatibility was determined ex vivo by assessing the degree of hemolysis, aggregation, and platelet adhesion. Results. The molecular weight of synthesized polymer was 33,000 g/mol with a polydispersity index of 1.3. When studying cell adhesion, no significant differences (p = 0.20) between the properties of the SIBS polymer (588 cells/mm2) and the properties of culture plastics (732 cells/mm2) were discovered. Cell adhesion for the ePTFE material was 212 cells/mm2. Percentage of dead cells on SIBS and ePTFE samples was 4.40 and 4.72% (p = 0.93), respectively, for culture plastic – 1.16% (p < 0.05). Cell proliferation on the ePTFE surface (0.10%) was significantly lower (p < 0.05) than for the same parameters for SIBS and culture plastic (62.04 and 44.00%). Implantation results (60 days) showed the formation of fibrous capsules with average thicknesses of 42 μm (ePTFE) and 58 μm (SIBS). Calcium content in the explanted samples was 0.39 mg/g (SIBS), 1.25 mg/g (ePTFE) and 93.79 mg/g (GA-xenopericardium) (p < 0.05). Hemolysis level of red blood cells after contact with SIBS was 0.35%, ePTFE – 0.40%, which is below positive control (p < 0.05). Maximum platelet aggregation of intact platelet-rich blood plasma was 8.60%, in contact with SIBS polymer – 18.11%, with ePTFE – 22.74%. Conclusion. In terms of hemocompatibility properties, the investigated SIBS polymer is not inferior to ePTFE and can be used as a basis for development of polymeric prosthetic heart valves.

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The authors declare that there are no conflicts of interest present.